螯合型表面活性剂强化黑麦草修复Cd污染水体

刁静茹; 赵保卫; 马锋锋; 蒋煜峰; 马驰宇; 张淋淋

PDF(500 KB)

中国环境科学 ›› 2020, Vol. 40 ›› Issue (5) : 2238-2245.

环境生态

螯合型表面活性剂强化黑麦草修复Cd污染水体

刁静茹, 赵保卫, 马锋锋, 蒋煜峰, 马驰宇, 张淋淋

作者信息 +

Chelating surfactant assisted phytoremediation of Cd contaminated water by ryegrass

DIAO Jing-ru, ZHAO Bao-wei, MA Feng-feng, JIANG Yu-feng, MA Chi-yu, ZHANG Lin-lin

Author information +

文章历史 +

摘要

采用水培试验，研究了新型螯合型表面活性剂N-十二酰基乙二胺三乙酸钠（LED3A）强化黑麦草修复Cd污染水体的效果，探讨了Cd或/和LED3A存在下黑麦草的生理响应以及LED3A对黑麦草除Cd性能的影响.结果表明：Cd显著抑制黑麦草生物量和光合色素含量，添加LED3A后抑制作用略有增强，CL100处理叶绿素和类胡萝卜素含量较CL0分别降低了15.50%和8.18%. LED3A对黑麦草的生长无显著影响，对黑麦草细胞的相对电导率（REC）有一定的提高，可使细胞膜渗透性增加以利于黑麦草对Cd的吸收.Cd胁迫下适量添加LED3A，能提高黑麦草植株超氧化歧化酶（SOD）和过氧化物酶（POD）活性，起到清除活性氧（ROS）、缓解Cd毒害和抑制丙二醛（MDA）积累的作用.最佳LED3A添加量下（50mg/L），黑麦草植株的总Cd累积量和转移系数较Cd单独处理时分别提高了74.39%和67.96%，Cd的去除率达到55.98%.研究表明添加LED3A可有效提高植物对Cd的修复效率.

Abstract

A novel chelating surfactant named N-lauroyl ethylenediamine triacetate (LED3A) assisted phytoremediation of Cd by ryegrass was studied under hydroponics conditions. Physiological responses of ryegrass, as well as the Cd removal efficiency, in Cd or LED3A alone and combination treatments were investigated. The results indicated that the biomass and photosynthetic pigments of ryegrass significantly declined under Cd stress. LED3A plus Cd addition would lead to slight increase of the inhibition mentioned above. The chlorophyll and carotenoid contents in ryegrass of CL100 treatment were decreased by 15.50% and 8.18% compared with CL0. No obvious changes of ryegrass biomass were observed with increasing LED3A concentration when LED3A was added alone. The relative electrical conductivity (REC) of ryegrass cells were increased by LED3A, which may increase the permeability of the cell membrane and hence promote Cd uptake by ryegrass. An appropriate quantity of LED3A could enhanced the activities of superoxide dismutase (SOD) and peroxide enzyme (POD) of ryegrass under Cd stress, suggesting a positive role of LED3A in scavenging reactive oxygen species (ROS), alleviating Cd toxicity and preventing malonyldialdehyde (MDA) accumulation. At optimal LED3A dosage (50mg/L), the total Cd accumulation amount and Cd translocation factor in ryegrass were increased by 74.39% and 67.96% compared with the Cd treatment alone. The optimal removal efficiency of Cd was reached 55.98%. The studies have shown that the addition of LED3A can effectively improve the phytoremediation efficiency of Cd in Cd contaminated water.

导出引用

刁静茹, 赵保卫, 马锋锋, 蒋煜峰, 马驰宇, 张淋淋. 螯合型表面活性剂强化黑麦草修复Cd污染水体[J]. 中国环境科学. 2020, 40(5): 2238-2245

DIAO Jing-ru, ZHAO Bao-wei, MA Feng-feng, JIANG Yu-feng, MA Chi-yu, ZHANG Lin-lin. Chelating surfactant assisted phytoremediation of Cd contaminated water by ryegrass[J]. China Environmental Science. 2020, 40(5): 2238-2245

中图分类号： X171.5

参考文献

[1] 陈卫平,杨阳,谢天,等.中国农田土壤重金属污染防治挑战与对策[J]. 土壤学报, 2018,55(2):261-272. Chen W P, Yang Y, Xie T, et al. Challenges and countermeasures for heavy metal pollution control in farmlands of China[J]. Acta PedologicaSinica, 2018,55(2):261-272.
[2] 中华人民共和国环境保护部,中华人民共和国国土资源部.全国土壤污染状况调查公报. 2014. State environmental protection administration of China, state land and resources administration of China. National bulletin of soil pollution survey, 2014.
[3] Zhao F J, Ma Y, Zhu Y G, et al. Soil contamination in China:Current status and mitigation strategies[J]. Environmental Science and Technology, 2015,49(2):750-759.
[4] 张子杨,郭瞻宇,管伟豆,等.硫磺和放线菌强化植物修复土壤镉污染[J]. 中国环境科学, 2019,39(5):2106-2114. Zhang Z Y, Guo Z Y, Guan W D, et al.Combination of sulphur and actinomycete to enhance the efficiency of phytoremediation in cadmium contamination soil[J]. China Environmental Science, 2019, 39(5):2106-2114.
[5] 周启星,宋玉芳.污染土壤修复原理与方法[M]. 北京:科学出版社, 2018:134-144. Zhou Q X, Song Y F. Remediation of contaminated soils:principles and methods[M]. Beijing:Science Press, 2018:134-144.
[6] 雒焕章,南忠仁,胡亚虎,等.不同螯合剂处理下杨树对土壤中Cd的吸收和富集效应[J]. 中国环境科学, 2013,33(3):461-465. Luo H Z, Nan Z R, Hu Y H, et al. Chelate-induced uptake and accumulation of Cd in soil by poplar[J]. China Environmental Science, 2013,33(3):461-465.
[7] Zhang H Z, Guo Q J, Yang J X, et al. Comparison of chelates for enhancing Ricinus communisL. phytoremediation of Cd and Pb contaminated soil[J]. Ecotoxicology and Environmental Safety, 2016,133(11):57-62.
[8] 韩少华,唐浩,黄沈发.重金属污染土壤螯合诱导植物修复研究进展[J]. 环境科学与技术, 2011,34(6G):157-163. Tang S H, Tang H, Huang S F. Review of Chelate-induced phytoremediation in heavy metal contaminated soil[J]. Environmental Science and Technology, 2011,34(6G):157-163.
[9] Evangelou M W H, Ebel M, Schaeffer A. Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity, and fate of chelating agents[J]. Chemosphere, 2007,68(6):989-1003.
[10] Doumett S, Lamperi L, Checchini L, et al. Heavy metal distribution between contaminated soil and Paulownia tomentosa, in a pilot-scale assisted phytoextraction study:influence of different complexing agents[J]. Chemosphere, 2008,72(10):1481-1490.
[11] 骆永明,吴龙华,宋静,等.重金属污染土壤的修复机制与技术发展[M]. 北京:科学出版社, 2016:8-11. Luo Y M, Wu L H, Song J, et al. Remediation mechanism and technological development of heavy metal polluted soil[M]. Beijing:Science Press, 2016:8-11.
[12] Wang X, Zhao J, Yao X, et al. Synthesis and properties of N-hexadecyl ethylenediamine triacetic acid[J]. Journal of Colloid and Interface Science, 2004,279(2):548-551.
[13] 王军,杨许召,李刚森.功能性表面活性剂制备与应用[M]. 北京:化学工业出版社, 2009:79-100. Wang J, Yang X S, Li G S. Synthesis and application of functional surfactants[M]. Beijing:Chemical Industry Press, 2009:79-100.
[14] 牛金平,韩亚明.螯合性表面活性剂的制备工艺与应用性能(V)-N-酰基ED3A的使用安全性[J]. 日用化学工业, 2008,38(2):121-124. Niu J P, Han Y M. Manufacturetechnologyandapplicationper formance of chelatingsur factant (V)-SafetyinapplicationofN-acylED3A[J]. China Surfactant Detergent and Cosmetics, 2008,38(2):121-124.
[15] Qiao H T, Zhao B W, Diao J R, et al. Removal of lead and zinc from contaminated soil by a novel chelating surfactant[J]. Clean-Soil, Air, Water, 2016,44(9):1191-1196.
[16] Zhao B W, Huang L P, Diao J R,et al. Remediation of copper(II)-cadmium(II) contaminated loess soil using a novel chelating surfactant[J]. Fresenius Environmental Bulletin, 2015,24(10b):3473-3478.
[17] Chen T R, Liu X Y, Zhang X Y, et al. Effect of alkyl polyglucoside and nitrilotriacetic acid combined application on lead/pyrene bioavailability and dehydrogenase activity in co-contaminated soils[J]. Chemosphere, 2016,154(7):515-520.
[18] 刘新.植物生理学实验指导[M]. 北京:中国农业出版社, 2015:24-27;59-68. Liu X. Plant physiology experiment guidance[M]. Beijing:China Agricultural Press, 2015:24-27;59-68.
[19] 冉烈,李会合,田秀英.外源NO对镉胁迫下东南景天生长和镉累积的影响[J]. 中国农学通报, 2012,28(19):60-64. Ran L, Li H H, Tian X Y. Effects of exogenous nitric oxide on growth and cadmium accumulation in Sedum alfrediiHance under Cd stress[J]. Chinese Agricultural Science Bulletin, 2012,28(19):60-64.
[20] Zhu L Z, Zhang M. Effect of rhamnolipids on the uptake of PAHs by ryegrass[J]. Environmental Pollution, 2008,156(1):46-52.
[21] 高彦征,朱利中,胡晨剑,等.Tween80对植物吸收菲和芘的影响[J]. 环境科学学报, 2004,24(4):713-718. Gao Y Z, Zhu L Z, Hu C J, et al. Effects of Tween 80 on plant uptake of phenanthrene and pyrene from water[J]. Acta Scientiae Circumstantiae, 2004,24(4):713-718.
[22] Almeida C M R, Dias A C, Mucha A P, et al. Influence of surfactants on the Cu phytoremediation potential of a salt marsh plant[J]. Chemosphere, 2009,75(2):135-140.
[23] Shahabivand S, Parvaneh A, Aliloo A A. Root endophytic fungus Piriformosporaindica affected growth, cadmium partitioning and chlorophyll fluorescence of sunflower under cadmium toxicity[J]. Ecotoxicology and Environmental Safety, 2017(11),145:496-502.
[24] Matsubara S, Krause G H, Seltmann M, et al. Lutein epoxide cycle, light harvesting and photoprotection in species of the tropical tree genus Inga[J]. Plant Cell and Environment, 2008,31(4):548-561.
[25] Ehsan S, Ali S, Noureen S, et al. Citric acid assisted phytoremediation of cadmium by Brassica napus L[J]. Ecotoxicology and Environmental Safety, 2014,106(8):164-172.
[26] 史广宇,李中义,张路,等.黑麦草对水体中镉-壬基酚复合污染的生理响应及修复[J]. 环境科学, 2018,39(10):4512-4518. Shi G Y, Li Z Y, Zhang L, et al. Physiological responses of ryegrass in cadmium-nonylphenol co-contaminated water and the phytor emediation effects[J]. Environmental Science, 2018,39(10):4512-4518.
[27] 闫志强,陈银萍,蘧苗苗,等.镉胁迫对紫花苜蓿幼苗生理特性和镉富集的影响[J]. 广西植物, 2019,39(2):218-227. Yan Z Q, Chen Y P, Qu M M, et al. Effects of Cd stress on physiological characteristics of alfalfa and its Cd enrichment[J]. Guihaia, 2019,39(2):218-227.
[28] 栾红艳,赵卫红,苗辉.Cd²⁺胁迫下中肋骨条藻细胞内多胺的生理响应[J]. 中国环境科学, 2015,35(5):1487-1494. Luan H Y, Zhao W H, Miao H. Polyamines response to Cd²⁺ stress and their physiological roles in SkeletonemaCostatum [J]. China Environmental Science, 2015,35(5):1487-1494.
[29] Ruley A T, Sharma N C, Sahi S V. Antioxidant defense in a lead accumulating plant, Sesbaniadrummondii[J]. Plant Physiology and Biochemistry, 2004,42(11):899-906.
[30] 冉文静,傅大放.Gallic acid和SDS对黑麦草体内重金属亚细胞及形态分布的影响[J]. 环境科学学报, 2010,30(11):2264-2269. Ran W J, Fu D F. Influence of gallic acid and SDS on the subcellular and chemical form distribution of heavy metals in Ryegrass (Loliumperenne L.)[J]. Acta Scientiae Circumstantiae, 2010,30(11):2264-2269.
[31] Muhammad D, Chen F, Zhao J, et al.Comparison of EDTA-and citric acid-enhanced phytoextraction of heavy metals in artificially metal contaminated soil by Typha Angustifolia [J]. International Journal of Phytoremediation, 2009,11(6):558-574.
[32] 周建民,党志,陈能场,等.NTA对玉米体内Cu、Zn的积累及化学形态的影响[J]. 农业环境科学学报, 2007,26(2):453-457. Zhou J M, Dang Zhi, Chen N C, et al. Influence of NTA on accumulation and chemical form of copper and zinc in maize[J]. Journal of Agro-Environment Science, 2007,26(2):453-457.