Simulation experiments on effects of lomefloxacin on denitrification process in water
DENG Lu, HE Jiang-tao, ZOU Hua, CHEN Cui-bai
Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China
Abstract:The removal of nitrate in aquatic environment depends on denitrification by microorganisms under anaerobic conditions. It has been found that the presence of antibiotics can affect the activity of denitrifying bacteria and denitrifying enzymes, and then affect denitrification. However, the relationships among these three factors and the potential influencing mechanisms remains unclear in water system. In this study, simulation experiments were carried out to determine the effects of antibiotic on denitrification process, denitrification bacteria and denitrification enzyme activities. In the experiments, lomefloxacin (LOM) was selected as a typical antibiotic and sodium acetate was used as a carbon source. The results showed that the denitrification rates exposed to 0, 1, 10 and 100μg/L LOM were 10.42, 8.83, 8.50 and 6.62mg/(L·d), and the nitrogen removal reached 79.5%, 71.1%, 70.0% and 66.8%, respectively. For 100μg/L LOM exposure, the inhibition rate on denitrifying bacteria was up to 30.5%. This suggested that LOM with a certain concentration could affect the microbial growth processes and denitrifying enzyme activity. Therefore, the inhibition of NO3--N transformation during denitrification was observed, which further led to the decrease in denitrification and total nitrogen removal rate. Moreover, the higher the initial LOM concentration, the more significant the inhibition effect (P<0.05). Further results demonstrated that effects of LOM on denitrification mainly functioned in the process of NO3--N reduction to NO2--N. With increasing initial LOM concentration, the growth rate and maximum increment of bacteria decreased, and the damage to the integrity of microbial cell membrane became worse. The transformation of NO3--N and NO2--N was related to denitrifying enzyme activity. LOM concentration had a significant influence on nitrate reductase activity. The higher the concentration, the greater the degree of inhibition. However, the activity of nitrite reductase was hardly affected LOM concentration. Although the above simulated experimental conditions did not completely matched with the real water, the results implied that the low concentration of antibiotics in environment water body is likely to affect the denitrification process by inhibiting the microbial population and activity as well as the denitrifying enzyme activity, and consequently reduce the denitrification rate and lead to the accumulation of nitrate pollution.
邓璐, 何江涛, 邹华, 陈翠柏. 洛美沙星对水中反硝化过程的影响模拟试验[J]. 中国环境科学, 2020, 40(7): 2934-2942.
DENG Lu, HE Jiang-tao, ZOU Hua, CHEN Cui-bai. Simulation experiments on effects of lomefloxacin on denitrification process in water. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 2934-2942.
Schaider L A, Rudel R A, Ackerman J M, et al. Pharmaceuticals, perfluorosurfactants, and other organic wastewater compounds in public drinking water wells in a shallow sand and gravel aquifer[J]. Science of the Total Environment, 2014,468:384-393.
[2]
D'Alessio M, Durso LM, Miller D N, et al. Environmental fate and microbial effects of monensin, lincomycin, and sulfamethazine residues in soil[J]. Environmental Pollution, 2019,246:60-68.
[3]
Shan J, Yang P P, Rahman M.M., et al. Tetracycline and sulfamethazine alter dissimilatory nitrate reduction processes and increase N2O release in the rice fields[J]. Environmental Pollution, 2018,242:788-796.
[4]
Yin G Y, Hou L J, Liu M, et al. Effects of multiple antibiotics exposure on denitrification process in the Yangtze Estuary sediments[J]. Chemosphere, 2017,171:118-125.
[5]
Chen A, Chen Y, Ding C, et al. Effects of tetracycline on simultaneous biological wastewater nitrogen and phosphorus removal[J]. RSC Advances, 2015,5:59326-59334.
[6]
Yi K X, Wang D B, Yang Q, et al. Effect of ciprofloxacin on biological nitrogen and phosphorus removal from wastewater[J]. Science of the Total Environment, 2017,605-606:368-375.
[7]
Underwood J C, Harvey R W, Metge D W, et al. Effects of the Antimicrobial Sulfamethoxazole on Groundwater Bacterial Enrichment[J]. Environmental Science & Technology, 2011,45:3096-3101.
[8]
Sun M M, Ye M, Liu K, et al. Dynamic interplay between microbial denitrification and antibiotic resistance under enhanced anoxic denitrification condition in soil[J]. Environmental Pollution, 2017,222:583-591.
[9]
Hou L J, Yin G Y, Liu M, et al. Effects of Sulfamethazine on Denitrification and the Associated N2O Release in Estuarine and Coastal Sediments[J]. Environmental Science & Technology, 2015,49:326-333.
[10]
Ahmad M, Vithanage M, Ki K, et al. Inhibitory Effect of Veterinary Antibiotics on Denitrification in Groundwater:A Microcosm Approach[J]. The Scientific World Journal, 2014:1-7.
[11]
Chen H B, Zhou Y F, Hu X Y, et al. Effects of chlortetracycline on biological nutrient removal from wastewater[J]. Science of the Total Environment, 2019,647:268-274.
[12]
邹高龙,刘志文,董洁平,等.环丙沙星在污水处理过程中的迁移转化及对污水生物处理的影响[J]. 环境科学学报, 2019,39(2):308-317. Zou G L, Liu Z W, Dong J P, et al. The transformation of ciprofloxacin in wastewater treatment and its impact on wastewater treatment[J]. Acta Scientiae Circumstantiae, 2019,39(2):308-317.
[13]
Rahman M M, Shan J, Yang P P, et al. Effects of long-term pig manure application on antibiotics, abundance of antibiotic resistance genes (ARGs), anammox and denitrification rates in paddy soils[J]. Environmental Pollution, 2018,240:368-377.
[14]
Costanzoa S D, Murbyb J, Bates J. Ecosystem response to antibiotics entering the aquatic environment[J]. Marine Pollution Bulletin, 2005, 51:218-223.
[15]
Zou H, He J T, He B N, et al. Sensitivity assessment of denitrifying bacteria against typical antibiotics in groundwater[J]. Environmental Science:Processes & Impacts, 2019,21(9):1570-1579.
[16]
Martinez L J, Li G, Chignell C F. Photogeneration of Fluoride by the Fluoroquinolone Antimicrobial Agents Lomefloxacin and Fleroxacin[J]. Photochemistry and Photobiology, 1997,65(3):599-602.
[17]
陈瑞骞,魏东斌,杜宇国. Fenton氧化法降解洛美沙星的研究[J]. 科学技术与工程, 2014,14(21):306-310. Chen R Q, Wei D B, Du Y G, et al. Degradation characteristics of lomefloxacin by fenton oxidation[J]. Science Technology and Engineering, 2014,14(21):306-310.
[18]
Zou Y, Lin M, Xiong W, et al. Metagenomic insights into the effect of oxytetracycline on microbial structures, functions and functional genes in sediment denitrification[J]. Ecotoxicology and Environmental Safety, 2018,161:85-91.
[19]
肖晶晶,郭萍,霍炜洁,等.反硝化微生物在污水脱氮中的研究及应用进展[J]. 环境科学与技术, 2019,32(12):97-102. Xiao J J, Guo P, Huo W J, et al. Application of denitrifying microbes to wastewater denitrification[J]. Environmental Science & Technology, 2019,32(12):97-102.
[20]
Xia S Q, Li J X, Wang R C, et al. Tracking composition and dynamics of nitrification and denitrification microbial community in a biofilm reactor by PeR-DGGE and combining FISH with flow cytometry[J]. Biochemical Engineering Journal, 2010,49(3):370-78.
[21]
Calderer M, Gibert O, Martí V, et al. Denitrification in presence of acetate and glucose for bioremediation of nitrate-contaminated groundwater[J]. Environmental Technology, 2010,31(7):799-814.
[22]
马娟,宋相蕊,李璐.碳源对反硝化过程NO2-积累及出水pH值的影响[J]. 中国环境科学, 2014,(10):2556-2561. Ma J, Song X R, Li L. Effect of carbon source on nitrite accumulation and pH value of effluent during denitrification process[J]. China Environmental Science, 2014,(10):2556-2561.
[23]
毕春雪,于德爽,杜世明,等.乙酸钠作为碳源不同污泥源短程反硝化过程亚硝酸盐积累特性[J]. 环境科学, 2019,40(2):783-790. Bi C X, Yu D S, Du S M, et al. Nitrite accumulation characteristics of partial denitrification in different sludge sources using sodium acetate as carbon source[J]. Environmental Science, 2019,40(2):783-790.
[24]
Liu X H, Lu S Y, Guo W, et al. Antibiotics in the aquatic environments:A review of lakes, China[J]. Science of the Total Environment, 2018,627:1195-1208.
[25]
Lin Y C, Yu T H, Lin C F. Pharmaceutical contamination in residential, industrial, and agricultural waste streams:Risk to aqueous environments in Taiwan[J]. Chemosphere, 2008,74(1):131-141.
[26]
Klaus Kümmerer. Antibiotics in the aquatic environment-A review-Part I[J]. Chemosphere, 2009,75:417-434.
[27]
崔亚丰,何江涛,苏思慧,等.某市典型地段地表水及地下水中氟喹诺酮类抗生素分布特征[J]. 环境科学, 2015,36(11):4060-4067. Cui Y F, He J T, Su S H, et al. Distribution characteristics of fluoroquinolones antibiotics in surface water and groundwater from typical areas in a city[J]. Environmental Science, 2015,36(11):4060-4067.
[28]
辛明秀,赵颖,周军,等.反硝化细菌在污水脱氮中的作用[J]. 微生物学通报, 2007,34(4):773-776. Xin M X, Zhao Y, Zhou J, et al. The application of denitrifying bacteria in denitrification of wastewater[J]. Microbiology China, 2007,34(4):773-776.
[29]
Zwietering M H, Jongenburger I, Rombouts F M, et al. Modeling of the bacterial-growth curve[J]. Applied and Environmental Microbiology, 1990,56(6):1875-1881.
[30]
郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J]. 中国环境科学, 2015,35(1):116-121. Zheng L X, Li J;Hu J W, et al. Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems[J]. China Environmental Science, 2015,35(1):116-121.
[31]
吴伟,刘道玉,瞿建宏,等.脱氮副球菌硝酸盐/亚硝酸盐还原酶的活性变化及对养殖水体中无机氮素的转化[J]. 农业环境科学学报, 2013,32(6):1244-1252. Wu W, Liu D Y, Qu J H, et al. The changes of nitrate and nitrite reductase activity of paracoccus denitrificans and its inorganic nitrogen removal for aquaculture water[J]. Journal of Agro-Environment Science, 2013,32(6):1244-1252.
[32]
李玲玲,刘晓萍,杨兆雪. 低C/N比污水反硝化过程中亚硝态氮累积特性研究[J]. 环境污染与防治, 2016,38(12):72-77. Li L L, Liu X P, Yang Z X. Study on nitrite-N accumulation characteristics during denitrification process in low C/N ratio sewage[J]. Environmental Pollution & Control, 2016,38(12):72-77.
[33]
董晓莹,彭党聪.不同碳氮比下污水反硝化过程中亚硝氮积累的特性研究[J]. 环境科学学报, 2017,37(9):3349-3355. Dong X Y, Peng D C. Nitrite accumulation in denitrification with different C/N ratios[J]. Acta Scientiae Circumstantiae, 2017,37(9):3349-3355.