Abstract：In this study, the impact of different sludge conditioning methods (polyacrylamide conditioning, Fe[III]/CaO conditioning, chemical acidification and bioleaching conditioning) on the streptomycin, gentamicin and kanamycin ARB in dewatered sludge cake and dewatering filtrate was investigated using heterotrophic plate counts. The results showed that PAM conditioning could not reduce the ARB in dewatered sludge cake and dewatering filtrate. Fe[III]/CaO conditioning and bioleaching conditioning not only reduced the amount of three kinds of ARB in dewatered sludge cake, but also completely eliminated the three ARB in dewatering filtrate. The chemical acidification conditioning reduced the ARB in dewatered sludge cake by 0.83~1.65 log units, but increased the ARB in dewatering filtrate by 0.26~0.39 log units. The removal of ARB in dewatered sludge cake significantly correlated with the degree of microbial cell destruction in conditioned sludge, implying that the destruction of microbial cells resulting from conditioning treatments was the main reason for the removal of ARB in dewatered sludge. Further study revealed that the amount of ARB rebounded during the natural storage of dewatered sludge cakes, which showed a certain environmental risk.
毛秋燕,赵栩宁,苏宇傲,等.不同预处理方式对剩余污泥中活菌菌群及ARGs的影响[J]. 中国环境科学, 2020,40(6):2537-2545.Mao Q Y, Zhao X N, Su Y A, et al. Impact of different pretreatments on ARGs and live microbial communities in excess sludge[J]. China Environmental Science, 2020,40(6):2537-2545.
[2]
陆孙琴,李轶,黄晶晶,等.污水处理厂二级出水中总异养菌群对6种抗生素的耐受性研究[J]. 环境科学, 2011,32(11):3419-3424.Lu S Q, Li z, Huang J J, et al. Antibiotic resistance of bacteria to 6antibiotics in secondary effluents of municipalwastewater treatment plants[J]. Environmental Science, 2011,32(11):3419-3424.
[3]
Munir M, Wong K, Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan[J]. Water Research, 2011,45(2):681-693.
[4]
刘吉宝,李亚明,吕鑑,等.污水处理厂不同工艺的污泥脱水效能分析及其影响因素研究[J]. 环境科学, 2015,36(10):3794-3800.Liu J B, Li Y M, Lü J, et al. Performance and factors analysis of sludge dewatering in different wastewater treatment processes,[J]. Environmental Science, 2015,36(10):3794-3800.
[5]
Lu Y, Zheng G Y, Wu W Z, et al. Significances of deflocculated sludge flocs as well as extracellular polymeric substances in influencing the compression dewatering of chemically acidified sludge[J]. Separation and Purification Technology, 2017,176:243-251.
[6]
邢奕,王志强,洪晨,等.芬顿试剂与DDBAC联合调理污泥的工艺优化[J]. 中国环境科学, 2015,35(4):1164-1172.Xing Y, Wang Z Q, Hong C, et al. Technological optimiazation of sludge conditioned by Fenton's reagent combined with surfactant[J]. China Environmental Science, 2015,35(4):1164-1172.
[7]
Zheng G Y, Lu Y, Wang D Z, et al. Importance of sludge conditioning in attenuating antibiotic resistance:Removal of antibiotic resistance genes by bioleaching and chemical conditioning with Fe[III]/CaO[J]. Water Research, 2019,152:61-73.
[8]
Tian Z, Zhang Y, Yu B, et al. Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic[J]. Water Research, 2016,98:261-269.
[9]
Luo G, Li B, Li L G, et al. Antibiotic resistance genes and correlations with microbial community and metal resistance genes in full-scale biogas reactors as revealed by metagenomic analysis[J]. Environmental Science and Technology, 2017,51(7):4069-4080.
[10]
操宏庆,章菲菲,李健,等.高温厌氧消化中底物浓度对病原指示微生物杀灭的影响[J]. 环境科学, 2014,35(3):1016-1020.Cao H Q, Zhang F F, Li J, et al. Effect of substrate concentration on pathogen indicators inactivation during thermophilic anaerobic digestion[J]. Environmental Science, 2014,35(3):1016-1020.
[11]
郑冠宇,王世梅,周立祥.污泥生物沥浸处理对病原物的杀灭效果影响[J]. 环境科学, 2007,28(7):1539-1542.Zheng G Y, Wang S M, Zhou L X. Elimination of pathogens in municipal sludge and tannery sludge by bioleaching approach[J]. Environmental Science, 2007,28(7):1539-1542.
[12]
Qi Y, Dentel S K, Herson D S. Increases in fecal coliform bacteria resulting from centrifugal dewatering of digested biosolids[J]. Water Research, 2007,41(3):571-580.
[13]
Hudson L O, Murphy C R, Spratt B G, et al. Differences in methicillin-resistant staphylococcus aureus strains isolated from pediatric and adult patients from hospitals in a large county in California[J]. Journal of Clinical Microbiology, 2012,50(3):573-579.
[14]
吴云辉,严丽娟,沈鹭英,等.混合型离子交换液相色谱-串联质谱法测定蜂蜜中5种氨基糖苷类抗生素残留[J]. 色谱, 2019,37(5):499-504.Wu Y H, Yan L J, Shen L Y, et al. Determination of five aminoglycoside residues in honey using mixed-mode ion exchange liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019,37(5):499-504.
[15]
Fu J J, Xia C J, Wang Y, et al. An investigation for the key role of surfactants in activated sludge dewatering[J]. Journal of Chemical Engineering of Japan, 2010,43(2):238-246.
[16]
Deneux-Mustin S, Lartiges B S, Villemin G, et al. Ferric chloride and lime conditioning of activated sludges:an electron microscopic study on resin-embedded samples[J]. Water Research, 2001,35(12):3018- 3024.
[17]
Liu F W, Zhou J, Wang D Z, et al. Enhancing sewage sludge dewaterability by bioleaching approach with comparison to other physical and chemical conditioning methods[J]. Journal of Environmental Sciences, 2012,24(8):1403-1410.
[18]
Zheng G Y, Zhou L X, Wang S M. An acid-tolerant heterotrophic microorganism role in improving tannery sludge bioleaching conducted in successive multibatch reaction systems[J]. Environmental Science and Technology, 2009,43(11):4151-4156.
[19]
Zheng G Y, Wang Z Y, Wang D Z, et al. Enhancement of sludge dewaterability by sequential inoculation of filamentous fungus Mucor circinelloides ZG-3 and Acidithiobacillus ferrooxidans LX5[J]. Chemical Engineering Journal, 2016,284:216-223.
[20]
Pei R, Kim S, Carlson K H, et al. Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)[J]. Water Research, 2006,40(12):2427-2435.
[21]
Guo X S, Liu J X, Xiao B Y. Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge[J]. Journal of Biotechnology, 2014, 188:130-135.
[22]
Brooks J P, Maxwell S L, Rensing C, et al. Occurrence of antibiotic-resistant bacteria and endotoxin associated with the land application of biosolids[J]. Canadian Journal of Microbiology, 2007, 53(5):616-622.
[23]
周俊,周立祥,黄焕忠.污泥胞外聚合物的提取方法及其对污泥脱水性能的影响[J]. 环境科学, 2013,34(7):2752-2757.Zhou J, Zhou L X, Huang H Z. Optimization of extracellular polymeric substance extraction method and its role in the dewaterability of sludge[J]. Environmental Science, 2013,34(7):2752- 2757.
[24]
Lu Y, Zhang C M, Zheng G Y, et al. Improving the compression dewatering of sewage sludge through bioacidification conditioning driven by Acidithiobacillus ferrooxidans:dewatering rate vs. dewatering extent[J]. Environmental Technology, 2019,40(24):3176- 3189.
[25]
Tong J, Liu J B, Zheng X, et al. Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment[J]. Bioresource Technology, 2016,217:37- 43.
[26]
Zhou W B, Lu Y, Jiang S, et al. Impact of sludge conditioning treatment on the bioavailability of pyrene in sewage sludge[J]. Ecotoxicology and Environmental Safety, 2018,163:196-204.
[27]
Li N, Sheng G P, Lu Y Z, et al. Removal of antibiotic resistance genes from wastewater treatment plant effluent by coagulation[J]. Water Research, 2017,111:204-212.
[28]
Gehr R. Comment on "UV Disinfection Induces a Vbnc State in Escherichia coli and Pseudomonas aeruginosa"[J]. Environmental Science and Technology, 2015,49(12):7501.
[29]
Guo M T, Kong C. Antibiotic resistant bacteria survived from UV disinfection:Safety concerns on genes dissemination[J]. Chemosphere, 2019,224:827-832.
[30]
Erkan M, Sanin F D. Can sludge dewatering reactivate microorganisms in mesophilically digested anaerobic sludge? Case of belt filter versus centrifuge[J]. Water Research, 2013,47(1):428-438.
