进水亚硝氮限制下Anammox去除氨氮研究

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (579 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要利用UASB反应器分别在降低进水亚硝氮/氨氮比（R）和停供亚硝氮条件下研究了Anammox体系运行特性.发现随着进水亚硝氮减少，亚硝氮与氨氮去除摩尔比减小，发生氨氮超量去除现象，即使进水无亚硝氮时也可去除氨氮.当R为1：2时，氨氮超量去除量达最大，均值为57.2mg/L；长期停供亚硝氮条件下氨氮能够稳定去除，平均去除量为45.6mg/L.停供亚硝氮后Anammox体系中微生物群落多样性增加，AnAOB、氨氧化菌和反硝化菌相对丰度均增加.其中AnAOB相对丰度从9.44%增长到13.26%；氨氧化菌相对丰度从3.29%增长到7.3%；反硝化菌相对丰度由0.54%增加到3.14%.研究表明，溶解氧是氨氮超量去除量的限制性因素，氨氮超量去除的途径包括：好氧氨氧化、厌氧氨氧化与部分内碳源反硝化.在微量溶解氧作用下，主要是氨氧化菌与厌氧氨氧化菌协同实现了氮的去除.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	牛晚霞
	袁林江
	有小龙
	牛泽栋

关键词 ：厌氧氨氧化, 氨氮超量去除, 氨氧化, 内碳源反硝化

Abstract：Operation characteristics of an anammox system under the conditions of constantly reducing influent NO₂^--N/NH₄⁺-N ratio (R) and even no nitrite feeding in the UASB were studied. It has been found that as the influent nitrite decreased, the removal ratio of nitrite to ammonium also declined. The phenomenon of ammonium excess removal occurred. Even if there was no nitrite in the influent, ammonium had been removed. When the R was 1:2, the excess removal of ammonium reached the maximum, with an average of 57.2mg/L. The ammonium was removed stably in the reactor even without any nitrite supply, with an average removal of 45.6mg/L. The microbial diversity increased and the abundance of ammonia oxidizing bacteria, anaerobic ammonia oxidizing bacteria, denitrifying bacteria increased in the system. The relative abundance of AnAOB increased from 9.44% to 13.26%, ammonia oxidizing bacteria and denitrifying bacteria, from 3.29% and 0.54% to 7.3% and 3.14%, respectively. This study shows that dissolved oxygen was the limiting factor for the excess removal of ammonium. The pathways of excess removal of ammonium included aerobic ammonia oxidation, anammox and partial endogenous denitrification. While with a small amount of dissolved oxygen, ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria removed ammonium collaboratively.

Key words： anammox ammonium excess removal ammonia oxidation endogenous denitrification

收稿日期: 2020-11-26

PACS:

X703

基金资助:国家自然科学基金资助项目（51878538）

通讯作者: 袁林江,教授,yuanlinjiang@xauat.edu.cn E-mail: yuanlinjiang@xauat.edu.cn

作者简介: 牛晚霞(1994-),女,甘肃天水人,西安建筑科技大学硕士研究生,主要从事生活污水脱氮研究.发表论文2篇.

引用本文:

牛晚霞, 袁林江, 有小龙, 牛泽栋. 进水亚硝氮限制下Anammox去除氨氮研究[J]. 中国环境科学, 2021, 41(7): 3212-3220. NIU Wan-xia, YUAN Lin-jiang, YOUXiao-long, NIU Ze-dong. Study on removal of ammonia nitrogen by Anammox with or free of nitrite nitrogen. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3212-3220.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I7/3212

[1]	Jetten M S M, Cirpus I, Kartal B, et al. 1994-2004:10Years of research on the anaerobic oxidation of ammonium[J]. Biochemical Society Transactions, 2005,33(1):119-123.
[2]	Joss A, Salzgeber D, Eugster J, et al. Full-Scale nitrogen removal from digester liquid with partial nitritation and Anammox in one SBR[J]. Environmental Science & Technology, 2009,43(14):5301-5306.
[3]	Star W R L V D, Abma W R, Blommers D, et al. Startup of reactors for anoxic ammonium oxidation:Experiences from the first full-scale Anammox reactor in Rotterdam[J]. Water Research, 2007,41(18):4149-4163.
[4]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[5]	Yu J J, Jin R C. The Anammox reactor under transient-state conditions:Process stability with fluctuations of the nitrogen concentration, inflow rate, pH and sodium chloride addition[J]. Bioresource Technology, 2012,119:166-173.
[6]	Yang J, Zhang L, Hira D, et al. High-rate nitrogen removal by the Anammox process at ambient temperature[J]. Bioresource Technology, 2011,102(2):672-676.
[7]	刘倩,郭文龙,康银花,等.进水基质对厌氧氨氧化反应器稳定性能的影响[J]. 广东农业科学, 2012,39(23):172-176. Liu Q, Guo W L, Kang Y H, et al. Performance of ASBR Anammox reactor by ratio change of influent concentration of NO2--N/NH4+-N[J]. Guangdong Agricultural Sciences, 2012,39(23):172-176.
[8]	Furukawa K, Rouse J D, Yoshida N, et al. Mass cultivation of anaerobic ammonium-axidizing sludge using a novel nonwoven biomass carrier[J]. Journal of Chemical Engineering of Japan, 2004, 36(10):1163-1169.
[9]	Jin R C, Xing B S, Yu J J, et al. The importance of the substrate ratio in the operation of the Anammox process in upflow biofilter[J]. Ecological Engineering, 2013,53:130-137.
[10]	Bi Z, Wan Yan D Q, Li X, et al. Biological conversion pathways of sulfate reduction ammonium oxidation in anammox consortia[J]. Frontiers of Environmental Science & Engineering, 2020,14(3):1-11.
[11]	唐崇俭,郑平.厌氧氨氧化膨胀污泥床反应器的化学计量学特性[J]. 中国环境科学, 2010,30(11):1446-1452. Tang C J, Zheng P. Stoichiometric characteristics of anammox expanded sludge bed reactor[J]. China Environmental Science, 2010, 30(11):1446-1452.
[12]	国家环境保护总局.水和废水监测分析方法(第四版)[M]. 北京:中国环境科学出版社, 2002:258-276. State Environmental Protection Administration. Water and waste water monitoring and analysis method (Fourth Edition)[M]. Beijing:China Environmental Science Press, 2002:258-276.
[13]	陈重军,张海芹,汪瑶琪,等.基于高通量测序的ABR厌氧氨氧化反应器各隔室细菌群落特征分析[J]. 环境科学, 2016,37(7):2652-2658. Chen C J, Zhang H Q, Wang Y Q, et al. Characteristics of microbial community in each compartment of ABR Anammox reactor based on high-throughput sequencing[J]. Environmental Science, 2016,37(7):2652-2658.
[14]	闫媛,黎力,王亚宜,等.采用高通量测序分析全程自养脱氮(CANON)系统不同脱氮效能下的微生物群落结构[J]. 北京工业大学学报, 2015,41(10):1485-1492. Yan Y, Li L, Wang Y Y, et al. Microbial community characteristics of a completely autotrophic nitrogen removal over nitrite (CANON) system based on high-throughput sequencing technology[J]. Journal of Beijing University of Technology, 2015,41(10):1485-1492.
[15]	Jetten M S M, Strous M, Pas-Schoonen K T v d, et al. The anaerobic oxidation of ammonium[J]. FEMS Microbiology Reviews, 1998, 22(5):421-437.
[16]	严锋,袁林江,王洋,等.亚硝氮停供及恢复供给对Anammox反应器中氮去除的影响[J]. 环境科学学报, 2017,37(12):4602-4609. Yan F, Yuan L, Wang Y, et al. Effect of stop feeding and resupplying of nitrite on nitrogen removal in an Anammox-UASB[J]. Acta Scientiae Circumstantiae, 2017,37(12):4602-4609.
[17]	Ali M, Chai L Y, Tang C J, et al. The increasing interest of Anammox research in China:Bacteria, process development, and application[J]. BioMed Research International, 2013,2013(6):134914.
[18]	Liu S, Yang F, Gong Z, et al. Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal[J]. Bioresource Technology, 2008,99(15):6817-6825.
[19]	秦永丽,蒋永荣,刘成良,等.硫酸盐型厌氧氨氧化反应器的启动特性[J]. 环境工程学报, 2015,9(12):5849-5854. Qin Y L, Jiang Y R, Liu C L, et al. Starting characteristics of sulfate-dependent anaerobic ammonium oxidation reactor[J]. Chinese Journal of Environmental Engineering, 2015,9(12):5849-5854.
[20]	刘福鑫.厌氧氨氧化微生物利用不同电子受体的可行性研究[D]. 苏州:苏州科技学院, 2014. Liu F X. The feasibility study of different electron acceptors by Anammox microorganisms[D]. Suzhou:Suzhou University of Science and Technology, 2014.
[21]	严锋.上升流速及基质供给对Anammox反应器脱氮的影响研究[D]. 西安:西安建筑科技大学, 2017. Yan F. Effect of upward-flow velocity and substrate supplying in an Anammox-UASB[D]. Xi'an:Xi'an University of Architecture and Technology, 2017.
[22]	Yang W H, Weber K A, Silver W L. Nitrogen loss from soil through anaerobic ammonium oxidation coupled to iron reduction[J]. Nature Geoence, 2012,5(8):538-541.
[23]	Waki M, Yasuda T, Fukumoto Y, et al. Effect of electron donors on anammox coupling with nitrate reduction for removing nitrogen from nitrate and ammonium[J]. Bioresource Technology, 2013,130:592-598.
[24]	Andrews S S. Iron storage in bacteria[J]. Advances in Microbial Physiology, 1998,40(40):281-351.
[25]	赵然.Anammox细菌铁还原代谢及群体感应现象的研究[D]. 大连:大连理工大学, 2016. Zhao R. Researches of Anammox iron reduction and quorum sensing phenomena[D]. Dalian:Dalian University of Technology, 2016.
[26]	雷欣,闫荣,慕玉洁,等.铁元素对厌氧氨氧化菌脱氮效能的影响[J/OL]. 化工进展:1-11[2020-12-29]. https://doi.org/10.16085/j.issn.1000-6613.2020-1178. Lei X, Yan R, Mu Y J, et al. Effect of iron on nitrogen removal efficiency of anaerobic ammonium oxidation bacteria[J/OL]. Chemical Industry and Engineering Progress:1-11[2020-12-29]. https://doi.org/10.16085/j.issn.1000-6613.2020-1178.
[27]	彭厦.铁、锰离子对SBBR厌氧氨氧化效能的影响研究[D]. 哈尔滨:哈尔滨工业大学, 2012. Peng X. Impact of ferrous iron and manganese ion on the performance of SBBR Anammox reactor[D]. Harbin:Harbin Institute of Technology, 2012.
[28]	Clément J-C, Shrestha J, Ehrenfeld J G, et al. Ammonium oxidation coupled to dissimilatory reduction of iron under anaerobic conditions in wetland soils[J]. Soil Biology & Biochemistry, 2005,37(12):2323-2328.
[29]	Shrestha J, Rich J J, Ehrenfeld J G, et al. Oxidation of ammonium to nitrite under iron-reducing conditions in wetland soils:Laboratory, field demonstrations, and push-pull rate determination[J]. Soil ence, 2009,174(3):156-164.
[30]	van de Graaf A A V, Robertson L A. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
[31]	Waki M, Yasuda T, Yokoyama H, et al. Nitrogen removal by co-occurring methane oxidation, denitrification, aerobic ammonium oxidation, and anammox[J]. 2009,84(5):977-985.
[32]	陈婷婷,郑平,胡宝兰.厌氧氨氧化菌的物种多样性与生态分布[J]. 应用生态学报, 2009,20(5):1229-1235. Chen T T, Zhen P, Hu B L. Species diversity and ecological distribution of anaerobic ammonium-oxidizing bacteria[J]. The journal of applied ecology, 2009,20(5):1229-1235.
[33]	李泽兵,刘帅,张鹤,等.厌氧氨氧化污泥异养反硝化的影响因素分析[J]. 环境工程, 2019,37(12):12-16,21. Li ZB, Liu S, Zhang H, et al. Research on influencing factors on heterotrophic denitrification of Anammox sludge[J]. Environmental Engineering, 2019,37(12):12-16,21.
[34]	李祥,林兴,王凡,等.厌氧氨氧化污泥中氨氧化的潜在电子受体[J]. 环境科学, 2017,38(7):2941-2946. Li X, Lin X, Wang F, et al. Ammonia oxidation with potential electron acceptor in Anammox sludge[J]. Environmental Science, 2017,38(7):2941-2946.
[35]	完颜德卿.Anammox培养物对氨与硫酸盐同步转化机制的研究[D]. 苏州:苏州科技大学, 2017. Wan Yan D Q. Study on the mechanism of simultaneous conversion of Anammox for ammonia and sulfate[D]. Suzhou:Suzhou University of Science and Technology, 2017.
[36]	Bodelier P L E, Libochant J A, Blom C W P M, et al. Dynamics of nitrification and denitrification in root-oxygenated sediments and adaptation of ammonia-oxidizing bacteria to low-oxygen or anoxic habitats[J]. Applied and Environmental Microbiology, 1996,62(11):4100-4107.
[37]	Dwyer D J, Camacho D M, Kohanski M A, et al. Antibiotic-induced bacterial cell death exhibits physiological and biochemical hallmarks of apoptosis[J]. Molecular Cell, 2012,46(5):561-572.
[38]	Zhang Z, Liu S. Insight into the overconsumption of ammonium by anammox consortia under anaerobic conditions[J]. Journal of Applied Microbiology, 2015,117(6):1830-1838.
[39]	Ma J, Wang Z, Yang Y, et al. Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454high-throughput pyrosequencing[J]. Water Research, 2013,47(2):859-869.
[40]	Simpson E H. Measurement of diversity[J]. Nature, 1949,163(4148):688-688.
[41]	Shannon C E. A mathematical theory of communication[J]. Bell System Technical Journal, 1949,27(4):379-423.
[42]	Shannon P, Markiel A, Ozier O, et al. Cytoscape:A software environment for integrated models of biomolecular interaction networks[J]. Genome Research, 2003,13(11):2498-2504.
[43]	Zart D, Bock E. High rate of aerobic nitrification and denitrification by Nitrosomonas eutropha grown in a fermentor with complete biomass retention in the presence of gaseous NO2 or NO[J]. Archives of Microbiology, 1998,169(4):282-286.
[44]	Pereira A D, Cabezas A, Et alhebehere C, et al. Microbial communities in anammox reactors:a review[J]. Environmental Technology Reviews, 2017,6(1):74-93.
[45]	Mi W, Zhao J, Ding X, et al. Treatment performance, nitrous oxide production and microbial community under low-ammonium wastewater in a CANON process[J]. Water Science and Technology, 2017,76(11/12):3468-3477.