Abstract:The tolerance of Anammox to organic matter in the presence of Fe3+ (2.24~7.84mg/L) was investigated by running four reactors (R1control, R2 2.24mg/L Fe3+, R3 4.48mg/L Fe3+, R4 7.84mg/L Fe3+). The mechanism was clarified by using 16SrRNA high-throughput sequencing technology and qPCR. The results showed that high NH4+-N and TN removal rates (> 90%) of all four reactors could be achieved at the influent COD concentration of 50 and 100mg/L, and the presence of Fe3+ did not show the obvious positive effect. As the influent COD concentration increased to 150 and 200mg/L, the performance of Anammox was inhibited and the positive effect of Fe3+ increased. When the influent COD concentration was 200mg/L, the addition of Fe3+ (7.84mg/L) increased the NH4+-N and TN removals from 61.3% and 79.8% (R1) to 71.2% and 84.7%, respectively. 16SrRNA high-throughput sequencing results indicated the decrease of Anammox bacteria and the proliferation of denitrifying bacteria in the presence of organic matter. The presence of Fe3+ could increase the abundance of Planctomycetes. Fe3+ had promoting effects on the abundance of Anammox 16S rRNA and functional gene hzsB by qPCR data analysis.
王海月, 彭玲, 毛念佳, 耿金菊, 任洪强, 许柯. 三价铁对有机物存在下厌氧氨氧化脱氮的影响[J]. 中国环境科学, 2021, 41(4): 1672-1680.
WANG Hai-yue, PENG Ling, MAO Nian-jia, GENG Jin-ju, REN Hong-qiang, XU Ke. Effect of Fe3+ on nitrogen removal of Anammox in the presence of organic matter. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1672-1680.
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