The contribution of Nitrosomonas europaea/Nitrosococcus mobilis lineage to the deamination in full-scale landfill leachate treatment systems
XIONG Ying1,2, XIANG Si1,2, CHENG Kai1
1. Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization for College of Resources and Environmental Engineering, Hubei University of Technology, Wuhan 430068, China; 2. Wuhan Cyano-King Environmental Technology Co. Ltd, Wuhan 430068, China
Abstract：In order to study the relationship between the microbial community structure and the deamination effect in landfill leachate treatment systems, 16S rRNA high-throughput sequencing technology was used to analyze the community structure of autotrophic ammonia oxidizing bacteria (AOB) in 15 full-scale landfill leachate treatment systems. Meanwhile, the annual change of the community structure of AOB in one of the treatment systems was monitored. Moreover, a 45-day simulation test using real landfill leachate was carried out to study the deamination activity. The results showed that Nitrosomonas was the dominant AOB genus in landfill leachate treatment systems, and its relative abundance was positively correlated with the deamination activity (P<0.05). Within the genus, the relative abundance of N. europaea, N. eutropha and N. halophila, which all belongs to the N. europaea/Nitrosococcus mobilis lineage, were positively correlated with deamination activity (P<0.05). The results indicated that the N. europaea/Nitrosococcus mobilis lineage was the dominant AOB group in the full-scale landfill leachate treatment systems, and greatly contributed to the deamination in landfill leachate.
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