Abstract：Zebrafish was selected as the model organism to explore the combined toxicity of TET and As on aquatic organisms. The results showed that the combined exposure of 50μg/L TET and 100μg/L As significantly increased gene expression of oxidative stress (GPx), inflammatory factors (TNF-α) and apoptosis factors (C-jun), and caused severer damage to the intestine and liver in zebrafish. The synergistic toxicity of 50 μg/L TET and 100 μg/L As was related to the inhibition of P-gp gene, which significantly increased the accumulation of As in intestinal tract and liver [(704.15±24.50)ng/g and (458.35±24.25ng/g)]. In addition, the results of this study indicated that the changes of intestinal permeability and microbial community structure had little effect on the synergistic toxicity of TET and As. The results of this study provided basic data for the evaluation of the combined effects of TET and As.
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