Interaction between benz[a]anthracene and pulmonary surfactant mixed phospholipid monolayers
GENG Ying-xue1,2, LI Man-tao3, CAO Yan1, ZHAO Qun1, LI Ying-jie1, LIU Dan1, SHI Wei-meng1
1. Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China; 2. Faculty of Civil and Hydraulic Engineering, Xichang University, Xichang 615013, China; 3. Faculty of Land Resources Engineering, Kunming University of Science and Technology, Kunming 650093, China
Abstract：To investigate the effect of polycyclic aromatic hydrocarbons on pulmonary surfactant (PS) monolayers, mixed phospholipids of 1,2-dipalmitoyl-sn-glycerin-3-phosphate (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycerine-3-phosphatidylcholine (POPC) were selected as PS models to study the interface chemistry reaction between benz[a]anthracene and DPPC/POPC mixed phospholipids. The results showed that benz[a]anthracene can significantly affect the phase transition of DPPC/POPC mixed phospholipid monolayers and weaken the stability of the monolayers. Furthermore, benz[a]anthracene disturbed the dense and ordered arrangement of phospholipid molecules. With the increase of the proportion of POPC, the adverse effects were aggravated. DPPC/POPC mixed micelles can enclose benz[a]anthracene promoting the dissolution of benz[a]anthracene.When the concentration of DPPC is 200mg/L, the apparent solubility of benz[a]anthracene is nearly three times that of aqueous solution and the solubilization was more obvious with the increase of POPC, which was mainly due to the fact that POPC was beneficial to the dispersion of micelles. We can draw a conclusion that benz[a]anthracene can inhibit the physiological function of PS, and PS can in turn affect the dissolution and migration of benz[a]anthracene, further increase the exposure risk of benz[a]anthracene. At the same time, the change of PS components caused by physiological conditions may lead to the difference of potential toxicity of benz[a]anthracene, and the lung injury of people with related lung diseases may be more serious.
耿迎雪, 李曼焘, 曹妍, 赵群, 李英杰, 刘丹, 师伟萌. 苯并[a]蒽与肺表面活性剂混合磷脂的相互作用[J]. 中国环境科学, 2021, 41(7): 3381-3389.
GENG Ying-xue, LI Man-tao, CAO Yan, ZHAO Qun, LI Ying-jie, LIU Dan, SHI Wei-meng. Interaction between benz[a]anthracene and pulmonary surfactant mixed phospholipid monolayers. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3381-3389.
Zhang Y, Zheng H, Zhang L, et al. Fine particle-bound polycyclic aromatic hydrocarbons (PAHs) at an urban site of Wuhan, central China:Characteristics, potential sources and cancer risks apportionment[J]. Environmental Pollution, 2019,246:319-327.
[2]
Zhu Y Y, Duan X L, Qin N, et al. Health risk from dietary exposure to polycyclic aromatic hydrocarbons (PAHs) in a typical high cancer incidence area in southwest China[J]. Science of the Total Environment, 2019,649:731-738.
[3]
金梦,王梓,黎玉清,等.石化企业周边儿童多环芳烃内暴露负荷的时间变异性[J]. 中国环境科学, 2018,38(5):345-352. Jin M, Wang Z, Li Y Q, et al. The research on the temporal variability of the internal exposure levels of polyeyclic aromatic hydrocarbons in children around the petrochemical enterprise[J]. China Environmental Science, 2018,38(5):345-352.
[4]
Li W, Wang C, Wang H Q, et al. Atmospheric polycyclic aromatic hydrocarbons in rural and urban areas of Northern China[J]. Environmental Pollution, 2014,192:83-90.
[5]
Ki-hyun K, Shamin A J, Ehsanul K, et al. A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects[J]. Environment International, 2013,60:71-80.
[6]
王超,张霖琳,刀谞,等.京津冀地区城市空气颗粒物中多环芳烃的污染特征及来源[J]. 中国环境科学, 2015,35(1):1-6. Wang C, Zhang L L, Dao X, et al. Pollution characteristics and source identification of polycyclic aromatic hydrocarbons in airborne particulates of Beijing-Tianjin-Hebei Region, China[J]. China Environmental Science, 2015,35(1):1-6.
[7]
韩凤.肺微血管内皮细胞屏障功能损伤与急性呼吸窘迫综合征[J]. 中国急救医学, 2015,35(11):1048-1053. Han F. The damage of pulmonary microvascular endothelial cell barrier and acute respiratory distress syndrome[J]. Chinese Journal of Critical Care Medicine, 2015,35(11):1048-1053.
[8]
Ursula M, Rolf D, Kathrin M. Lung surfactant for pulmonary barrier restoration in patients with COVID-19pneumonia[J]. Frontiers in Medicine, 2020,7:254-258.
[9]
Wang R J, Guo Y, Liu H J, et al. The effect of chitin nanoparticles on surface behavior of DPPC/DPPG langmuir monolayers[J]. Journal of Colloid & Interface Science, 2018,519:186-193.
[10]
Zhao Q, Li Y J, Chai X L, et al. Interaction of inhalable volatile organic compounds and pulmonary surfactant:Potential hazards of VOCs exposure to lung[J]. Journal of Hazardous Materials, 2019,369:512-520.
[11]
Davies M J, Birkett J W, Kotwa M, et al. The impact of cigarette/e-cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions[J]. Surface and Interface Analysis, 2017,49(7):654-665.
[12]
Victoria G, Alan N H, Anthony D P. Regulation of lung surfactant phospholipid synthesis and metabolism[J]. Biochimica et Biophysica Acta, 2013,1831(2):448-458.
[13]
Eduardo G, Libero L, Eva S, et al. Effect of hydrophilic and hydrophobic nanoparticles on the surface pressure response of DPPC monolayers[J]. The Journal of Physical Chemistry C, 2011,115:21715-21722.
[14]
董声焕.肺表面活性物质基础与临床[M]. 北京:人民军医出版社, 2012:18-24. Dong S H. Pulmonary surfactant basic and clinical studies[M]. Beijing:People's Military Medical Press, 2012:18-24.
[15]
Li J H, Sun R G, Hao C C, et al. The behavior of the adsorption of cytochrome C on lipid monolayers:A study by the Langmuir-Blodgett technique and theoretical analysis[J]. Biophysical Chemistry, 2015, 205:33-40.
[16]
Anna S K, Trojan S, Cwiklik L, et al. Modeling lung surfactant interactions with Benzo[a] pyrene[J]. Chemistry-A European Journal, 2017,23(22):5307-5316.
[17]
Roy Z, Leslie L, Lia A. Crystalline domain structure and cholesterol crystal nucleation in single hydrated DPPC:Cholesterol:POPC bilayers[J]. Journal of the American Chemical Society, 2010,132:9920-9927.
[18]
Juan T B. Thermodynamic behaviour of mixed films of an unsaturated and a saturated polar lipid.(Oleic acid-stearic acid and POPC-DPPC)[J]. Colloids Interfaces, 2018,2:17.
[19]
Agnieszka O, Michal Z, Martina R, et al. Mixed DPPC/POPC monolayers:All-atom molecular dynamics simulations and langmuir monolayer experiments[J]. Biochimica et Biophysica Acta, 2016, 1858:3120-3130.
[20]
Jorge B D L S, Soren H, Zane B, et al. Compositional and structural characterization of monolayers and bilayers composed of native pulmonary surfactant from wild type mice[J]. Biochimica et Biophysica Acta, 2013,1828:2450-2459.
[21]
Notter R H. Lung surfactants:Basic science and clinical applications[M]. New York:Marcel Dekker Inc, 2000:171-206.
[22]
Ashton M R, Postle A D, Hall M A, et al. Phosphatidylcholine composition of endotracheal tube aspirates of neonates and subsequent respiratory disease[J]. Archives of Disease in Childhood, 1992,67:378-382.
[23]
Schmidt R, Meier U, Yabut-Perez M, et al. Alteration of fatty acid profiles in different pulmonary surfactant phospholipids in acute respiratory distress syndrome and severe pneumonia[J]. American Journal of Respiratory and Critical Care Medicine, 2001,163(1):95-100.
[24]
Sosnowski T R, Kolinski M, Gradon L. Interactions of Benzo[a] pyrene and diesel exhaust particulate matter with the lung surfactant system[J]. Annals of Occupational Hygiene, 2011,55(3):329-338.
[25]
Beata K, Anna S-K, Jacek K. The role of DPPG in lung surfactant exposed to benzo[a] pyrene[J]. Environmental science:Processes & Impacts, 2019,21(3):438-445.
[26]
Zhao Q, Li Y J, Chai X L, et al. Interaction of pulmonary surfactant with silica and polycyclic aromatic hydrocarbons:Implications for respiratory health[J]. Chemosphere, 2019,222:603-610.
[27]
Zhao Q, Wang Q, Li Y, et al. Influence of volatile organic compounds (VOCs) on pulmonary surfactant monolayers at air-water interface:Implication for the pulmonary health[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2019,562:402-408.
[28]
Eftaiha A, Qaroush A K, Kayed G, et al. Morphological and interaction characteristics of surface active ionic liquids and palmitic acid in mixed monolayers[J]. Chem. Phys. Chem., 2020,21(16):1858-1865.
[29]
Mladenova K, Petrova S D, Georgiev G A, et al. Interaction of Bestrophin-1with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in surface films[J]. Colloids and Surfaces B:Biointerfaces, 2014,122:432-438.
[30]
徐琳桢,赵群,田森林,等.重金属离子对肺表面活性物质单层膜的影响[J]. 中国环境科学, 2020,40(2):857-864. Xu L Z, Zhao Q, Tian S L, et al. Effect of heavy metal ions on the surface activity of pulmonary surfactant monolayer membrane[J]. China Environmental Science, 2020,40(2):857-864.
[31]
Wang F F, Liu J F, Zeng H B. Interactions of particulate matter and pulmonary surfactant:Implications for human health[J]. Advances in Colloid and Interface Science, 2020,284:102244.
[32]
Lilland N S, Simonsen A C, Duelund L, et al. Polyaromatic hydrocarbons do not disturb liquid-liquid phase coexistence, but increase the fluidity of model membranes[J]. Chemistry and Physics of Lipids, 2014,184:18-24.
[33]
梁超,乔俊琴,葛欣,等.保留时间两点校正反相高效液相色谱法测定持久性有机污染物的正辛醇-水分配系数[J]. 色谱, 2014, 32(4):426-432. Liang C, Qiao J Q, Ge X, et al. Determination of n-octanol/water partition coefficients for persistent organic pollutants by reversed-phase high performance liquid chromatography with dual-point retention time correction[J]. Chinese Journal of Chromatography, 2014,32(4):426-432.
[34]
Agnieszka O, Pauline D, Tereza D, et al. Properties of lipid models of lung surfactant containing cholesterol and oxidized lipids:A mixed experimental and computational study[J]. Langmuir, 2020,36:1023-1033.
[35]
Zaborowska M, Broniatowski M, Wydro P, et al. Structural modifications of lipid membranes exposed to statins-Langmuir monolayer and PM-IRRAS study[J]. Journal of Molecular Liquids, 2020,313:113570.
[36]
Ye X Q, Hao C C, Yang J J, et al. Influence of modified silica nanoparticles on phase behavior and structure properties of DPPC monolayers[J]. Colloids and Surfaces B:Biointerfaces, 2018,172:480-486.
[37]
Huynh L, Perrot N, Beswick V, et al. Structural properties of POPC monolayers under lateral compression:Computer simulations analysis[J]. Langmuir, 2014,30:564-573.
[38]
Zhang Z Y, Hao C C, Qu H J, et al. Studied on the dynamic adsorption process of Lycium barbarum polysaccharide in the POPC/DPPC monolayers[J]. Colloids and Surfaces B:Biointerfaces, 2019,178:38-43.
[39]
Zhao Q, Li Y J, Chai X L, et al. Interaction of nano carbon particles and anthracene with pulmonary surfactant:The potential hazards of inhaled nanoparticles[J]. Chemosphere, 2019,215:746-752.
[40]
Zhao J, Wang Z, Mashayekhi, et al. Pulmonary surfactant suppressed phenanthrene adsorption on carbon nanotubes through solubilization and competition as examined by passive dosing technique[J]. Environment Science & Technology, 2012,46:5369-5377.
[41]
Yamamoto H, Liljestrand H M. Partitioning of selected estrogenic compounds between synthetic membrane vesicles and water:Effects of lipid components[J]. Environment Science & Technology, 2004, 38:1139-1147.
