Abstract：In order to investigate species diversity of bdelloid rotifer in Guangdong Province, 98 samples from different habitats in Guangdong Province were collected, and 66 species (including subspecies) of bdelloid rotifers were recorded belonged to 3 orders, 4 families, 12 genera. Fifty-six species of bdelloid rotifer were identified in terrestrial environment, including 42 species in bryophyte habitats, 40 species in deciduous habitats, 12 species in lichen habitats, and 15species in aquatic habitats. The species number of bdelloid rotifer in terrestrial habitats was significantly more than that in aquatic habitats. To verify the accuracy of morphological identification, 37 COI gene sequences of 16 species belonged to 4genera were obtained, and the genetic distance among them ranged from 8.59% to 23.17%. The COI gene sequences of Adinet cuneata and Philodina rapida were first reported in the world. The total content (71.2%) of adenine (A) and thymine (T) was higher than that (28.8%) of guanine (G) and cytosine(C). The cluster results of phylogenetic tree were consistent with those of morphological species identification; the species with similar morphology belonged to the same genus have higher homology. The results showed that the combination of morphological identification and DNA barcoding technology could improve the accuracy of species identification for bdelloid rotifers, explore their phylogenetic position at molecular level, and promote the species diversity and ecological research of bdelloid rotifers.
王庆, 李莹, 汪文博, 杨宇峰. 广东省蛭态轮虫物种多样性及其系统发育研究[J]. 中国环境科学, 2021, 41(9): 4367-4377.
WANG Qing, LI Ying, WANG Wen-bo, YANG Yu-feng. Species diversity and phylogenetic analysis of bdelloid rotifers in Guangdong Province. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4367-4377.
Fontaneto D, Barraclough T G. Do species exist in asexuals? Theory and evidence from bdelloid rotifers[J]. Integrative and Comparative Biology, 2015,55(2):253-263.
[2]
Ricci C. Bdelloid rotifers:‘sleeping beauties’ and ‘evolutionary scandals’, but not only[J]. Hydrobiologia, 2017,796(1):277-285.
[3]
Welch D B M, Meselson M. Evidence for the evolution of bdelloid rotifers without sexual reproduction or genetic exchange[J]. Science, 2000,288(5469):1211-1215.
[4]
Gladyshev E A, Meselson M, Arkhipova I R. Massive horizontal gene transfer in bdelloid rotifers[J]. Science, 2008,320(5880):1210-1213.
[5]
Wilson C G, Sherman P W. Anciently asexual bdelloid rotifers escape lethal fungal parasites by drying up and blowing away[J]. Science, 2010,327(5965):574-576.
[6]
Flot J F, Hespeels B, Li X, et al. Genomic evidence for ameiotic evolution in the bdelloid rotifer Adineta vaga[J]. Nature, 2013,500(7463):453-457.
[7]
Fontaneto D. Long-distance passive dispersal in microscopic aquatic animals[J]. Movement Ecology, 2019,7(1):10.
[8]
Hebert P D N, Cywinska A, Ball S L, et al. Biological identifications through DNA barcodes[J]. Proceeding of Royal society of London. Biological Science, 2003,270(1512):313-321.
[9]
Schenk J, Fontaneto D. Biodiversity analyses in freshwater meiofauna through DNA sequence data[J]. Hydrobiologia, 2020,847:2597-2611.
[10]
项贤领,陶媛媛,席贻龙.轮虫种群遗传结构及系统地理格局研究进展[J]. 生态学杂志, 2015,34(7):2048-2057.Xiang X L, Tao Y Y, Xi Y L. Review on phylogeographical pattern and population genetic structure of rotifer[J]. Chinese Journal of Ecology, 2015,34(7):2048-2057.
[11]
Fontaneto D, Iakovenko N S, Eyres I, et al. Cryptic diversity in the genus Adineta Hudson & gosse, 1886(Rotifera:Bdelloidea:Adinetidae):a DNA taxonomy approach[J]. Hydrobiologia, 2011,662:27-33.
[12]
Iakovenko N S, Smykla J, Convey P, et al. Antarctic bdelloid rotifers:diversity, endemism and evolution[J]. Hydrobiologia, 2015,761:5-43.
[13]
Wang Q, Yang Y F, Chen J F. Impact of environment on the spatio-temporal distribution of rotifers in the tidal Guangzhou segment of the Pearl River Estuary, China[J]. International Review of Hydrobiology, 2009,94(6):688-705.
[14]
梁迪文,王庆,魏南,等.广州市不同类型水体轮虫群落结构的时空变动及与理化因子间的关系[J]. 湖泊科学, 2017,29(6):1433-1443.Liang D W, Wang Q, Wei N, et al. Spatial and temporal variation in rotifer community structure and the response to environmental factors among different water bodies in Guangzhou City[J]. Journal of Lake Sciences, 2017,29(6):1433-1443.
[15]
黄珂,李慧明,徐少林,等.不同水力调度模式的两座调水型水库轮虫群落结构的比较[J]. 湖泊科学, 2019,31(3):853-861.Huang K, Li H M, Xu S L, et al. Comparative analysis of rotifer community structures in two pumped-storage reservoirs with different retention times[J]. Journal of Lake Sciences, 2019,31(3):853-861.
[16]
王家楫.中国淡水轮虫志[M]. 北京:科学出版社, 1961.Wang J J. Freshwater rotifers of China[M]. Beijing:Science Press, 1961.
[17]
Donner J. Ordnung Bdelloidea (Rotatoria, Radertiere) Bestimmungsbü cher zur Bodenfauna Europas 6[M]. Berlin:Akademie-Verl, 1965.
[18]
Ricci C, Melone G. Key to the identification of the genera of bdelloid rotifers[J]. Hydrobiologia, 2000,418:73-80.
[19]
Jersabek C D, Leitner M F. The rotifer world catalog[EB/OL]. http://www.rotifera.hausdernatur.at/, 2021-01-05.
[20]
Xia X. DAMBE6:New tools for microbial genomics, phylogenetics and molecular evolution[J]. Journal of Heredity, 2017,108:431-437.
[21]
李莹,王庆,魏南,等.蛭态轮虫中国新记录种及其研究展望[J]. 水生生物学报, 2020,44(2):423-428.Li Y, Wang Q, Wei N, et al. Newly recorded species of bdelloid rotifer in China and research prospects[J]. Acta Hydrobiologica Sinica, 2020,44(2):423-428.
[22]
Zeng Y, Wei N, Wang Q, et al. Bdelloid rotifers (Rotifera, Bdelloidea) of China:diversity and new records[J]. ZooKeys, 2020,941:1-23.
[23]
Kaya M, Herniou E A, Barraclough T G, et al. Inconsistent estimates of diversity between traditional and DNA taxonomy in bdelloid rotifers[J]. Organisms Diversity and Evolution, 2009,9(1):3-12.
[24]
Fontaneto D, Kaya M, Herniou E A, et al. Extreme levels of hidden diversity in microscopic animals (Rotifera) revealed by DNA taxonomy[J]. Molecular Phylogenetics and Evolution, 2009,53(1):182-189.
[25]
Robeson M S, King A J, Freeman K R, et al. Soil rotifer communities are extremely diverse globally but spatially autocorrelated locally[J]. Proceedings of the National Academy of Sciences, 2011,108(11):4406-4410.
[26]
Kuczyńska-Kippen N. The use of bdelloids in reference to rotifer biocoenotic indices as an indicator of the ecological state of small field water bodies:The effect of macrophytes, shading and trophic state of water[J]. Ecological Indicators, 2018,89:576-583.
[27]
Hinz C, Ahlrichs W H, Bininda-Emonds O R P. Immediate and heritable costs of desiccation on the life history of the bdelloid rotifer Philodina roseola[J]. Organisms Diversity & Evolution, 2018,18(4):399-406.
[28]
Sommer S, Fontaneto D, Ozgul A. Demographic processes underlying fitness restoration in bdelloid rotifers emerging from dehydration[J]. Freshwater Biology, 2019,64(7):1295-1302.
[29]
Song M O, Lee C H. A new and five rare bdelloids from Korea[J]. Zootaxa, 2017,4242(3):529-547.
[30]
Song M O, Min G S. A new species and ten new records of bdelloid rotifers from Korea[J]. Zootaxa, 2015,3964(2):211-227.
[31]
鲍蕾,牛翠娟,马蕊,等.四种臂尾轮虫线粒体COI基因部分序列及系统发育关系[J]. 动物学研究, 2003,24(3):200-204.Bao L, Niu C J, Ma R, et al. Sequences and phylogeny of partial mitochondrial COⅠ gene from four brachionus species[J]. Zoological Research, 2003,24(3):200-204.
[32]
程双怀,魏世祥,张逸,等.基于ND4和ND5基因序列研究八种臂尾轮虫的系统关系和分类地位[J]. 水产学报, 40(12):1823-1829.Cheng S H, Wei S X, Zhang Y, et al. Phylogenetic relationship and taxonomic status of eight Brachionus species (rotifera) inferred from ND4 and ND5 genes[J]. Journal of Fisheries of China, 2016,40(12):1823-1829.
[33]
Örstan A. Taxonomic morphology of the genus Adineta (Rotifera:Bdelloidea:Adinetidae) with a new species from a suburban garden[J]. Zootaxa, 2018,4524(2):187-199.
[34]
Nowell R W, Almeida P, Wilson C G, et al. Comparative genomics of bdelloid rotifers:Insights from desiccating and nondesiccating species[J]. PLoS Biology, 2018,16(4):e2004830.
[35]
Jaturapruek R, Fontaneto D, Meksuwan P, et al. Planktonic and periphytic bdelloid rotifers from Thailand reveal a species assemblage with a combination of cosmopolitan and tropical species[J]. Systematics and Biodiversity, 2018,16(2):128-141.
