Abstract：Piperazine-degrading strain PIPA-6was isolated from the soil around a pharmaceutical plant in this study, the strain could grow in medium with piperazine as the sole energy source, and the degradation rate for 100mg/L of piperazine reached 100% within 30h. Strain PIPA-6 was finally identified as Paenarthrobacter nicotinovorans based on the analysis of morphological observation, physiological and biochemical tests, as well as 16s rDNA sequencing. Strain PIPA-6 could grow in a wide range of temperatures (10~40℃) and pH (5~10), with excellent sodium salt tolerance (50g/L) and high piperazine resistance (300mg/L). The optimum degradation was achieved at 35℃, pH,8. The degradation efficiency of the strain could be significantly improved by adding organic carbons.Simulation experiments showed that strain PIPA-6played a vital role in piperazine degradation in 50L simulation tank, the parameter of chemical oxygen demand(COD) and the content of ammonium nitrogen in pharmaceutical wastewater were significantly reduced(P<0.05).Degradation rate of strain PIPA-6 for piperazine was up to 98% in 30d. Metagenomics analysis confirmed that the strain could successfully colonize in the wastewater and shape new microbial community structure compared with the initial one. The strain obtained in this study enriched the resource of the piperazine-degrading strains, and the experimental results provided a preliminary theoretical basis for the development and practical application of the strain.
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