铜对蚯蚓转化牛粪过程抗生素抗性基因的影响

田雪力; 李仲瀚; 杨凤霞; 韩秉君; 张克强

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中国环境科学 ›› 2022, Vol. 42 ›› Issue (10) : 4688-4695.

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铜对蚯蚓转化牛粪过程抗生素抗性基因的影响

田雪力, 李仲瀚, 杨凤霞, 韩秉君, 张克强

作者信息 +

Effects of copper on antibiotic resistance genes during earthworm conversion of cow dung

TIAN Xue-li, LI Zhong-han, YANG Feng-xia, HAN Bing-jun, ZHANG Ke-qiang

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文章历史 +

摘要

以赤子爱胜蚓（Eisenia fetida）为试验蚓种，设定不同Cu浓度梯度，研究蚯蚓转化牛粪过程中Cu对ARGs消长的影响.结果表明，不同Cu浓度处理下，ARGs累积总丰度呈现出CF （未添加Cu）-1降低至4.33×10^-1copies/16S copies.低、中浓度Cu的添加显著提高了样品中ARGs的总丰度水平（P<0.05）.通过研究不同类型ARGs在不同时间阶段的变化，发现低、高浓度Cu处理组（T1、T3）在蚯蚓转化过程中提高了tet-ARGs、erm-ARGs和str-ARGs的丰度，而中浓度Cu处理组（T2）中tet-ARGs和str-ARGs丰度降低.冗余分析（RDA）得出pH值显著影响蚯蚓转化过程中ARGs相对丰度的变化（P<0.05），且TOC、TN和Cu对ARGs的消长也发挥着重要作用.蚯蚓转化可加速牛粪中ARGs丰度的削减，一定程度上能够减少其环境风险，但牛粪中Cu的残留可导致蚯蚓转化过程中部分ARGs的富集，因此亟需进一步优化蚯蚓转化处理工艺.

Abstract

In this study, Eisenia fetida was used as the experimental earthworm, different Cu concentration gradients were set to study the effect of Cu on ARGs growth during earthworm conversion of cow dung. Results revealed that, under different Cu concentrations, the total abundance of ARGs showed a trend of CF (without Cu addition) -1 to 4.33×10^-1 copies/16S copies. The addition of low and medium concentrations of Cu significantly increased the total abundance of ARGs in the samples (P<0.05). By studying the changes of different ARG subtypes in different time stages, we found that the low and high concentration of Cu treatment groups (T1, T3) increased the abundances of tet-ARGs, erm-ARGs and str-ARGs during earthworm conversion, while the abundances of tet-ARGs and str-ARGs decreased in the medium-concentration Cu treatment group (T2). Redundancy analysis (RDA) showed that pH significantly affected the relative abundance of ARGs during earthworm conversion (P<0.05), and TOC, TN, and Cu also played an important role in ARGs variation. Earthworm conversion can accelerate the reduction of ARGs in cow dung and reduce its environmental risk to a certain extent, but the residual Cu in cow dung would lead to the enrichment of some ARGs during earthworm conversion of cow dung, thus it was urgent to further optimize the treatment process of earthworm conversion.

导出引用

田雪力, 李仲瀚, 杨凤霞, 韩秉君, 张克强. 铜对蚯蚓转化牛粪过程抗生素抗性基因的影响[J]. 中国环境科学. 2022, 42(10): 4688-4695

TIAN Xue-li, LI Zhong-han, YANG Feng-xia, HAN Bing-jun, ZHANG Ke-qiang. Effects of copper on antibiotic resistance genes during earthworm conversion of cow dung[J]. China Environmental Science. 2022, 42(10): 4688-4695

中图分类号： X750

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