Abstract：In this study, Eisenia fetida was used as the experimental earthworm, different Cu concentration gradients were set to study the effect of Cu on ARGs growth during earthworm conversion of cow dung. Results revealed that, under different Cu concentrations, the total abundance of ARGs showed a trend of CF (without Cu addition) -1 to 4.33×10-1 copies/16S copies. The addition of low and medium concentrations of Cu significantly increased the total abundance of ARGs in the samples (P<0.05). By studying the changes of different ARG subtypes in different time stages, we found that the low and high concentration of Cu treatment groups (T1, T3) increased the abundances of tet-ARGs, erm-ARGs and str-ARGs during earthworm conversion, while the abundances of tet-ARGs and str-ARGs decreased in the medium-concentration Cu treatment group (T2). Redundancy analysis (RDA) showed that pH significantly affected the relative abundance of ARGs during earthworm conversion (P<0.05), and TOC, TN, and Cu also played an important role in ARGs variation. Earthworm conversion can accelerate the reduction of ARGs in cow dung and reduce its environmental risk to a certain extent, but the residual Cu in cow dung would lead to the enrichment of some ARGs during earthworm conversion of cow dung, thus it was urgent to further optimize the treatment process of earthworm conversion.
田雪力, 李仲瀚, 杨凤霞, 韩秉君, 张克强. 铜对蚯蚓转化牛粪过程抗生素抗性基因的影响[J]. 中国环境科学, 2022, 42(10): 4688-4695.
TIAN Xue-li, LI Zhong-han, YANG Feng-xia, HAN Bing-jun, ZHANG Ke-qiang. Effects of copper on antibiotic resistance genes during earthworm conversion of cow dung. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(10): 4688-4695.
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