1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China; 2. Key Laboratory of Yellow River Water Environment in Gansu Province, Lanzhou 730070, China; 3. Changchun Water Group Co. Ltd, Changchun 130000, China
Abstract：To eliminate the abundances of ARGs in sludge vermicompost, this study aimed to reveal the underlying effects of earthworms on the active bacterial community structure and their ARGs involved in vermicomposting systems for sludge recycling. For this, vermicomposting with and without earthworms was set up in parallel. Moreover, the dyeing pretreatment for samples with propidium monoazide (PMA) was adopted to screen out the DNA of active bacteria. The results showed that the electrical conductivity of sludge vermicompost was significantly increased by 82.5% (P<0.05), the degradation rate of organic matter was increased by 5.2% (P<0.05). Compared with the control treatment, the abundance of Actinobacteria significantly increased by 65.6% (P<0.05), while the abundance of Firmicutes and Bacteroidetes significantly decreased by 74.7% and 34.6%, respectively. Meanwhile, vermicomposting led to the selected ARGs abundances of tetM, sul1, sul2, ermB and ermF significantly decreased by 66.5%, 82.8%, 72.8% and 77.6% (P<0.05), while the abundance of ermB significantly increased by 5.7times (P<0.05) in active bacteria, compared to the counterpart. The abundance of intI1gene in vermicompost products significantly reduced by 67.2% compared with the control treatment. The total absolute abundance of ARGs was 4.19×1013 copies/g, and the total removal rate of ARGs was 82.6%, 45.4% higher than that of the counterpart. This study suggests that earthworms can reduce the abundance of dominant hosts of ARGs by modifying the active microbial community structure of sludge, thus reducing the associated dissemination risks of the spread of ARGs.
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