Bacterial diversity and community composition in the Phragmites australis rhizosphere by cometabolism
HONG Zhi-feng1, ZHANG Ni-chen1, A Dan2, QIU Rong-liang1,3,4, LIN Qing-qi3,4, NI Zhuo-biao3,4, CHAO Yuan-qing1,5
1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China; 2. Engineering and Technology Research Center for Agricultural Land Pollution Integrated Prevention and Control of Guangdong Higher Education Institutes, College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 3. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; 4. Guangdong Provincial Key Laboratory of Agricultural &Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; 5. Guangdong Provincial Key Laboratory for Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China
Abstract：p-hydroxybenzoic acid (PHA), p-coumaric acid (PCA), caffeic acid (CA), and ferulic acid (FA) were selected as the representative phenolic root exudates (PREs), p-tert-butylphenol (PTBP) was chosen as the typical alkylphenol, and common reed (Phragmites australis) was used as the test plant. High-throughput sequencing and bioinformatics analyses were applied to investigate the variation of bacterial diversity and community by PRE-PTBP interaction in Phragmites australis rhizosphere. PREs could effectively improve the rhizosphere bacterial activity and accelerate the organic carbon degradation. However, the PTBP biodegradation could be promoted by monophenol PREs (PHA, PCA and FA), but not by polyphenol PREs (CA). On the one hand, PREs could decide the dominant bacterial phyla as carbon source: Proteobacteria and Bacteroidetes with high carbon source utilization ability increased by 15.34% and 4.73% in +PRE treatments, respectively; whereas Actinobacteria and Firmicutes with better detoxification/tolerance capacity increased by 15.92% and 9.99% in -PRE treatments, respectively. On the other hand, the functional structure of PREs could determine the functional bacterial genus: monophenol PREs (PHA, PCA and FA) are conducive to enrich monophenol-degrading bacteria, like Pseudomonas, accounting for 1.45%~4.02%; polyphenol PREs (CA) are favored to increase the abundance of polyphenol-degrading bacteria, like Novosphingobium, accounting for 3.71%. Furthermore, simple structure of PREs is beneficial for the enrichment of ASVs. The closer the PRE structure are, the more similar the ASV species will be. The community composition of rhizosphere bacteria in different treatments is not a random phenomenon, but a directional adjustment of rhizosphere microbiome by exudates released from plants.
洪志锋, 张旎晨, 阿丹, 仇荣亮, 林庆祺, 倪卓彪, 晁元卿. 共代谢作用下芦苇根际细菌多样性与群落组成[J]. 中国环境科学, 2022, 42(4): 1812-1818.
HONG Zhi-feng, ZHANG Ni-chen, A Dan, QIU Rong-liang, LIN Qing-qi, NI Zhuo-biao, CHAO Yuan-qing. Bacterial diversity and community composition in the Phragmites australis rhizosphere by cometabolism. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1812-1818.
Capaldo A, Gay F, Valiante S, et al. Endocrine-disrupting effects of nonylphenol in the newt, Triturus carnifex (Amphibia, Urodela)[J]. Comparative Biochemistry and Physiology Part C, 2012,155(2):352-358.
[2]
Ismail N, Wee S Y, Aris A Z. Bisphenol A and alkylphenols concentrations in selected mariculture fish species from Pulau Kukup, Johor, Malaysia[J]. Marine Pollution Bulletin, 2018,127:536-540.
[3]
Nsenga Kumwimba M, Meng F G. Roles of ammonia-oxidizing bacteria in improving metabolism and cometabolism of trace organic chemicals in biological wastewater treatment processes:a review[J]. Science of the Total Environment, 2019,659:419-441.
[4]
Xie X M, Liao M, Jing Y, et al. Influence of root-exudates concentration on pyrene degradation and soil microbial characteristics in pyrene contaminated soil[J]. Chemosphere, 2012,88(10):1190-1195.
[5]
Lakshmanan V, Selvaraj G, Bais H P. Functional soil microbiome:belowground solutions to an aboveground problem[J]. Plant Physiology, 2014,166(2):689-700.
[6]
Leigh M B, Fletcher J S, Fu X, et al. Root turnover:an important source of microbial substrates in rhizosphere remediation of recalcitrant contaminants[J]. Environmental Science & Technology, 2002,36(7):1579-1583.
[7]
Toyama T, Furukawa T, Maeda N, et al. Accelerated biodegradation of pyrene and benzo[a]pyrene in the Phragmites australis rhizosphere by bacteria-root exudate interactions[J]. Water Research, 2011,45(4):1629-1638.
[8]
Donnelly P K, Hegde R S, Fletcher J S. Growth of PCB-degrading bacteria on compounds from photosynthetic plants[J]. Chemosphere, 1994,28(5):981-988.
[9]
A Dan, Fujii D, Soda S, et al. Removal of phenol, bisphenol A, and 4-tert-butylphenol from synthetic landfill leachate by vertical flow constructed wetlands[J]. Science of the Total Environment, 2017,578:566-576.
[10]
A Dan, Zhang N C, Qiu R L, et al. Accelerated biodegradation of p-tert-butylphenol in the Phragmites australis rhizosphere by phenolic root exudates[J]. Environmental and Experimental Botany, 2020,169:103891-103896.
[11]
Gao H X, Ding A W, Tang Y P, et al. Chemical constituents from the rhizomas of Phragmites communis [J]. Chinese Journal of Natural Medicines, 2009,7:196-198.
[12]
张凯煜,谷洁,王小娟,等.微生物有机肥对樱桃园土壤细菌群落的影响[J]. 中国环境科学, 2019,39(3):1245-1252. Zang K Y, Gu J, Wang X J, et al. Effects of bio-organic fertilizer on the soil bacterial community in a cherry orchard[J]. China Environmental Science, 2019,39(3):1245-1252.
[13]
周雅心,王晓彤,王广磊,等.炉渣与生物炭施加对稻田土壤细菌多样性及群落组成的影响[J]. 中国环境科学, 2020,40(3):1213-1223. Zhou Y X, Wang X T, Wang G L, et al. Effect of the slag and biochar application on bacterial diversity and community composition of paddy field[J]. China Environmental Science, 2020,40(3):1213-1223.
[14]
Khan S, Afzal M, Iqbal S, et al. Plant-bacteria partnerships for the remediation of hydrocarbon contaminated soils[J]. Chemosphere, 2013,90(4):1317-1332.
[15]
Jitendra M, Rachna S, Arora N K. Alleviation of heavy metal stress in plants and remediation of soil by rhizosphere microorganisms[J]. Front Microbiol, 2017,241:124950-124957.
[16]
Zeng Y X, Yang Z, Grebmeier J M, et al. Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea[J]. Polar biology, 2012,35(1):117-129.
[17]
Fu W G, Wang Y X, Wei W, et al. Species diversity and functional prediction of soil bacterial communities in constructed wetlands with different plant conditions[J]. Current Microbiology, 2019,76:338-345.
[18]
Li T C, Fan Y C, Cun D S, et al. Treatment performance and microbial response to dibutyl phthalate contaminated wastewater in vertical flow constructed wetland mesocosms[J]. Chemosphere, 2019,246:125635- 125645.
[19]
滕泽栋,李敏,朱静,等.野鸭湖湿地芦苇根际微生物多样性与磷素形态关系[J]. 环境科学, 2017,38(11):4589-4597. Teng Z D, Li M, Zhu J, et al. Effects of soil microbial diversity on the phosphate fraction in the rhizosphere of Phragmites australis communis in the Yeyahu wetland in Beijing, China[J]. Environmental Science, 2017,38(11):4589-4597.
[20]
Chen Y M, Ding Q B, Chao Y Q, et al. Structural development and assembly patterns of the root-associated microbiomes during phytoremediation[J]. Science of the Total Environment, 2018,644:1591-1601.
[21]
Adegboye M F, Babalola O O. Isolation and identification of potential antibiotic producing rare actinomycetes from rhizospheric soils[J]. Journal of Human Ecology, 2016,56(1/2):31-41.
[22]
A Álvarez, Yañez M L, Benimeli C S, et al. Maize plants (Zea mays) root exudates enhance lindane removal by native Streptomyces strains[J]. International Biodeterioration and Biodegradation, 2012,66(1):14-18.
[23]
Hai H, Daisuke I, Naonori M, et al. Characterization of novel 4-n-butylphenol-degrading Pseudomonas Veronii strains isolated from rhizosphere of giant duckweed, Spirodela polyrrhiza [J]. Japanese Journal of Water Treatment Biology, 2013,45(2):83-92.
[24]
潘声望,雷志华,吴云霄,等.苏丹草根分泌物在有机氯农药降解过程中的作用[J]. 中国环境科学, 2017,37(8):3072-3079. Pan S W, Lei Z H, Wu Y X, et al. Effect of exudates from sorghum sudanense grass roots on degradation of organochlorine pesticides in soils[J]. China Environmental Science, 2017,37(8):3072-3079.
[25]
Toyama T, Sato Y, Inoue D, et al. Biodegradation of bisphenol A and bisphenol F in the rhizosphere sediment of Phragmites australis [J]. Journal of Bioscience and Bioengineering, 2009,108(2):147-150.
[26]
Lu T, Ke M J, Jin Y J, et al. Rhizosphere microorganisms can influence the timing of plant flowering[J]. Microbiome, 2018,6:231- 242.
[27]
Zhalnina K, Louie K, Hao Z, et al. Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly[J]. Nature Microbiology, 2018,3:470-480.
