The diversity of culturable bacteria in the eastern edge of Kumtag Desert, and the characteristic of their radiation-resistance and anti-oxidation, including the correlation and coupling of these capacities
LIU Yang1,2,4, WANG Xin-yue5, CHEN Tuo1,2,4, ZHANG Gao-sen3,4, WU Fa-si6, ZHANG Wei3,4, LIU Guang-xiu3,4, WU Yu-jie1,2,4, XU Ye-teng1,2,4, TIAN Mao3,4
1. State Key Laboratory of Cryospheric Sciences, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 4. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, China; 5. College of Life Science, Shanghai Normal University, Shanghai 201499, China; 6. National Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Department of Conservation Research, Dunhuang Academy, Dunhuang 736200, China
Abstract：This study investigated the phylogenetic diversity of culturable bacteria in the eastern edge of Kumtag Desert, and then screened the radiation-resistant and oxidant-resistant strains via their survival rate after radiation and oxidation treatments. The correlations between their survival rates under the two treatments were also used to find the coupling relationship between the two capacities of bacteria. The results showed that pH, TOC, and TN were the key factors that influenced the community structure of culturable bacteria in the desert environment. In total, 105 bacteria strains were isolated from the eastern edge of the Kumtag Desert affiliated to the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Actinobacteria was the most dominant phylum in culturable bacteria in both abundance (9.96×105CFU/g) and species richness (72 strains from 9 genera). The culturable bacterial strains from the phyla Actinobacteria, Firmicutes, and Proteobacteria, were found to have both two capabilities to resist radiation and oxidation, but not bacterial strains from the phylum Bacteroidetes. The strains with D10 dose (with survival rate greater than 10%) were higher than 100J/m2 of UV-C radiation and accounted for 21.3% of the total culturable bacteria. The proportion of the anti-oxidant strains with D10 dose higher than 10mmol/L H2O2 accounted for 70.2%. The study showed that the two capabilities of culturable bacteria have positive correlation characteristics. Three strains from the actinobacteria, K4-10, K5-3, and K2-40, have very high capabilities to resist both radiation and oxidation.
刘阳, 王馨悦, 陈拓, 章高森, 武发思, 张威, 刘光琇, 吴玉洁, 徐业腾, 田茂. 可培养细菌多样性及抗辐射-抗氧化相关性特征——以库姆塔格沙漠东缘为例[J]. 中国环境科学, 2021, 41(12): 5921-5932.
LIU Yang, WANG Xin-yue, CHEN Tuo, ZHANG Gao-sen, WU Fa-si, ZHANG Wei, LIU Guang-xiu, WU Yu-jie, XU Ye-teng, TIAN Mao. The diversity of culturable bacteria in the eastern edge of Kumtag Desert, and the characteristic of their radiation-resistance and anti-oxidation, including the correlation and coupling of these capacities. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5921-5932.
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