Abstract:Yeasts were isolated by membrane filtration flat culture from Nam Co Lake. Identifications of the yeasts were based on sequence analysis of the ITS region, combined with traditional classification method. Moreover, bioinformatical and statistical methods were used to determine the correlationships between diversity of culturable yeast and environmental factors. The result showed that a total of 1067 yeast isolates were identified, belonging to 27 genera and 45 species, and including two previously undescribed taxa. Among them, Vishniacozyma victoriae and Naganishia adeliensis were the dominated species. Furthermore, NMDS analysis showed that the β diversity of the culturable yeast communities in different sites of Nam Co Lake was significantly different. Statistical analysis indicated that the richness of culturable yeast were negatively correlated to lake pH, electric conductivity (EC), and total dissolved salt (TDS). Meanwhile, the richness was positively correlated to total nitrogen (TN) and ammonia nitrogen (NH3-N). Additionally, the number of yeast species, Shannon-Wiener index, and Simpson index were negatively correlated to total phosphorus (TP). Interestingly, pH played a crucial role in the formation of yest community structures in Nam Co Lake. As a whole, the yeast community in Nam Co Lake showed high species richness, and the community structures differed in different sites, influenced by a variety of environmental factors.
王艳红, 郝兆, 郭小芳, 德吉. 纳木错夏季酵母菌多样性及其影响因素[J]. 中国环境科学, 2021, 41(11): 5361-5371.
WANG Yan-hong, HAO Zhao, GUO Xiao-fang, De-ji. Analysis on yeast diversity and the influencing factors during summertime in Nam Co Lake. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(11): 5361-5371.
王苏民,窦鸿身.中国湖泊志[M]. 北京:科学出版社, 1998.Wang S M, Dou H S. Records of lakes in China[M]. Beijing:Science Press, 1998.
[2]
Wetzel R G. Limnology:lake and river ecosystems[M]. Salt Lake City:Academic Press, 2001.
[3]
Wang M, Liang J, Hu L. Distribution of GDGTs in lake surface sediments on the Tibetan Plateau and its influencing factors[J]. Sci China-Earth Sci, 2016,59:961-974.
[4]
陈兆进,丁传雨,朱静亚,等.丹江口水库枯水期浮游细菌群落组成及影响因素研究[J]. 中国环境科学, 2017,37(1):336-344.Chen Z J, Ding C Y, Zhu J Y, et al. Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou Reservoir[J]. China Environmental Science, 2017,37(1):336-344.
[5]
Allison S D, Martiny J B H. Resistance, resilience, and redundancy in microbial communities[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008,105(1):11512-11519.
[6]
吴庆龙,江和龙.中国湖泊微生物组研究[J]. 中国科学院院刊, 2017,32(3):273-279.Wu Q L, Jiang H L. China lake microbiome project[J]. Bulletin of Chinese Academy of Sciences, 2017,32(3):273-279.
[7]
李娜英,韩智勇,王双超,等.多污染源作用下填埋场地下水微生物群落分析[J]. 中国环境科学, 2020,40(11):4900-4910.Li N Y, Han Z Y, Wang S C, et al. Impacts of different pollution sources on the microbial community in groundwater at municipal solid waste landfill sites[J]. China Environmental Science, 2020,40(11):4900-4910.
[8]
董明华,李治滢,周斌,等.云南高原湖泊杞麓湖冬季可培养酵母菌多样性分析[J]. 微生物学报, 2016,56(4):603-613.Dong M H, Li Z Y, Zhou B, et al. Diversity of cultivable yeast in Qilu Lake in winter[J]. Acta Microbiologica Sinica, 2016,56(4):603-613.
[9]
Medeiros A O, Missagia B S, Brandão L R, et al. Water quality and diversity of yeasts from tropical lakes and rivers from the Rio Doce basin in southeastern Brazil[J]. Brazilian Journal of Microbiology, 2012,43(4):1582-1594.
[10]
魏伟,王丽,周平,等.安徽铜陵地区河流生态系统健康的多指标评价[J]. 中国环境科学, 2013,33(4):691-699.Wei W, Wang L, Zhou P, et al. Multi-variable assessment of river ecosystem health in Tongling of Anhui Province[J]. China Environmental Science, 2013,33(4):691-699.
[11]
Hagler A N. Yeasts as indicator of environmental quality[M]. Berlin:Springer, 2006.
[12]
Nagahama T. Yeast biodiversity in freshwater, marine and deep-sea environments[M]. Berlin:Springer, 2006.
[13]
Brandão L R, Medeiros A O, Duarte M C, et al. Diversity and antifungal susceptibility of yeasts isolated by multiple-tube fermentation from three freshwater lakes in Brazil[J]. J. Water Health, 2010,8(2):279-289.
[14]
Arvanitidou M, Kanellou K, Vagiona D G. Diversity of Salmonella spp. and fungi in northern Greek rivers and their correlation to fecal pollution indicators[J]. Environ. Res., 2005,99(2):278-284.
[15]
de Almeida J M. Yeast community survey in the Tagus estuary[J]. FEMS Microbiol. Ecol., 2005,53(2):295-303.
[16]
李治滢,周新丽,周斌,等.云南程海湖冬季酵母菌多样性及胞外酶活性研究[J]. 菌物学报, 2017,36(2):177-185.Li Z Y, Zhou X L, Zhou B, et al. Diversity and extracellular enzymes of yeasts from Chenghai Lake in winter[J]. Mycosystema, 2017,36(2):177-185.
[17]
郭小芳,李治滢,董明华,等.云南高原湖泊抚仙湖酵母菌空间分布及其与环境因子的关系[J]. 湖泊科学, 2016,28(2):358-369.Guo X F, Li Z Y, Dong M H, et al. Spatial dynamics of yeast community and its relationship to environmental factors in Lake Fuxian, Yunnan Province[J]. Journal of Lake Sciences, 2016,28(2):358-369.
[18]
Russo G, Libkind D, Sampaio J P, et al. Yeast diversity in the acidic RioAgrio Lake Caviahue volcanic environment (Patagonia, Argentina)[J]. FEMS Microbiology Ecology, 2008,65:415-424.
[19]
Brandão L R, Libkind D, Vaz A B, et al. Yeasts from an oligotrophic lake in Patagonia (Argentina):Diversity, distribution and synthesis of photoprotective compounds and extracellular enzymes[J]. FEMS Microbiology Ecology, 2011,76(1):1-13.
[20]
Libkind D, Moliné M, Sampaio J P, et al. Yeasts from high-altitude:influence of UV radiation[J]. FEMS Microbiology Ecology, 2009, 69(3):353-362.
[21]
时玉,孙怀博,刘勇勤,等.青藏高原淡水湖普莫雍错和盐水湖阿翁错湖底沉积物中细菌群落的垂直分布[J]. 微生物学通报, 2014, 41(11):2379-2387.Shi Y, Sun H B, Liu Y Q, et al. Vertical distribution of bacterial community in sediments of freshwater lake Puma Yumco and saline lake AWongco on the Tibetan Plateau[J]. Microbiology China, 2014, 41(11):2379-2387.
[22]
孙殿超,龚平,王小萍,等.拉萨河全氟化合物的时空分布特征研究[J]. 中国环境科学, 2018,38(11):4298-4306.Sun D C, Dong P, Wang X P, et al. Special distribution and seasonal variation of perfluoroalkyls substances in Lhasa River Basin, China[J]. China Environmental Science, 2018,38(11):4298-4306.
[23]
万玮,肖鹏峰,冯学智,等.卫星遥感监测近30年来青藏高原湖泊变化[J]. 科学通报, 2014,59(8):701-714.Wan W, Xiao P F, Feng X Z, et al. Monitoring lake changes of Qinghai-Tibetan Plateau over the past 30years using satellite remote sensing data[J]. Chin. Sci. Bull., 2014,59(8):701-714.
[24]
刘金波,孔维栋,王君波,等.纳木错湖水体固碳微生物数量、群落结构及其驱动因子[J]. 生态学报, 2019,39(8):2772-2783.Liu J B, Kong W D, Wang J B, et al. Abundance, community structure, and the driving factors of Carbon fixing microorganisms in the Nam Co Lake[J]. Acta Ecologica Sinica, 2019,39(8):2772-2783.
[25]
杜娟,文莉娟,苏东生.青藏高原不同深度湖泊无冰期湖气温差及湖表辐射与能量平衡特征模拟分析[J]. 高原气象, 2020,39(6):1181-1194.Du J, Wen L J, Su D S. Analysis of simulated temperature difference between lake surface and air and energy balance of three alpine lakes with different depths on the Qinghai-Xizang Plateau during the ice-free period[J]. Plateau Meteorology, 2020,39(6):1181-1194.
[26]
陈飞,蔡强国,孙莉英.青藏高原纳木错流域冰雪融水径流量估算[J]. 中国水土保持科学, 2016,14(2):127-136.Chen F, Cai G Q, Sun L Y. Estimation of meltwater runoff from glaciers and snow cover in Nam Co basin, Tibetan Plateau[J]. Science of Soil and Water Conservation, 2016,14(2):127-136.
[27]
王永胜,孙继彬,王彦,等.西藏纳木错西岸班戈县新吉乡早白垩世的石珊瑚[J]. 古生物学报, 2020,59(4):452-466.Wang Y S, Sun J B, Wang Y, et al. Early Cretaceous scleractinian corals from Xenkyer, Baingoin, Tibet[J]. Acta Palaeontologica Sinica, 2020,59(4):452-466.
[28]
刘晓波,康世昌,刘勇勤,等.青藏高原纳木错湖细菌群落特征及其与高山湖泊的对比[J]. 冰川冻土, 2008,30(6):1041-1047.Liu X B, Kang S C, Liu Y Q, et al. Microbial community structures in the Nam Co Lake, Tibetan Plateau, and comparison with other alpine lakes[J]. Journal of Glaciology and Geocryology, 2008,30(6):1041-1047.
[29]
陆美美,周石硚,何霞.青藏高原湖泊蒸发估算方法的比较研究——以纳木错为例[J]. 冰川冻土, 2017,39(2):281-291.Lu M M, Zhou S Q, He X. A comparison of the ormulas for estimation of the lake evaporation on the Tibetan Plateau:Taking Lake Nam Co as an example[J]. Journal of Glaciology and Geocryology, 2017, 39(2):281-291.
[30]
黄磊,王君波,朱立平,等.纳木错水温变化及热力学分层特征初步研究[J]. 湖泊科学, 2015,27(4):711-718.Huang L, Wang J B, Zhu L P, et al. Water temperature and characteristics of thermal stratification in Nam Co, Tibet[J]. Journal of Lake Sciences, 2015,27(4):711-718.
[31]
王君波,朱立平,Gerhard Daut,等.西藏纳木错水深分布及现代湖沼学特征初步分析[J]. 湖泊科学, 2009,21(1):128-134.Wang J P, Zhu L P, Daut G, et al. Bathymetric survey and modern limnological parameters of Nam Co, central Tibet[J]. Journal of Lake Sciences, 2009,21(1):128-134.
[32]
严亚萍,李治滢,董明华,等.云南阳宗海酵母菌种群结构及产胞外酶测试[J]. 微生物学报, 2013,53(11):1205-1212.Yan Y P, Li Z Y, Dong M H, et al. Yeasts from Yangzonghai Lake in Yunnan (China):Diversity and extracellular enzymes[J]. Acta Microbiologica Sinica, 2013,53(11):1205-1212.
[33]
孙立夫,张艳华,裴克全.一种高效提取真菌总DNA的方法[J]. 菌物学报, 2009,28(2):299-302.Sun L F, Zhang Y H, Pei K Q. A rapid extraction of genomic DNA from fungi[J]. Mycosystema, 2009,28(2):299-302.
[34]
White T J, Bruns T D, Lee S B, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics//PCR Protocols:A Guide to Methods and Applications[M]. San Diego:Academic Press, 1990:315-322.
[35]
Kurtzman C P, Robnett C J. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit(26S) ribosomal DNA partial sequences[J]. Antonie Van Leeuwenhoek, 1998,73(4):331-371.
[36]
罗正明,刘晋仙,暴家兵,等.五台山亚高山土壤真菌海拔分布格局与构建机制[J]. 生态学报, 2020,40(19):7009-7017.Luo Z M, Liu J X, Bao J B, et al. Elevational distribution patterns and assembly mechanisms of soil fungal community in Mount Wutai, Shanxi, China[J]. Acta Ecologica Sinica, 2020,40(19):7009-7017.
[37]
白梅,侯强川,孙志宏,等.健康蒙古族人肠道中乳酸菌和双歧杆菌多样性[J]. 微生物学通报, 2019,46(10):2697-2709.Bai M, Hou Q C, Sun Z H, et al. Diversity of lactic acid bacteria and Bifidobacterium in the intestine of healthy Mongolians[J]. Microbiology China, 2019,46(10):2697-2709.
[38]
郝兆,王艳红,郑艳艳,等.羊卓雍措水体可培养酵母菌多样性及其与理化因子相关性[J]. 微生物学报, 2021,61(5):1269-1286.Hao Z, Wang Y H, Zheng Y Y, et al. Analysis on culturable yeast diversity and its relationship with environmental factors in Yamzhog Yumco Lake[J]. Acta Microbiologica Sinica, 2021,61(5):1269-1286.
[39]
李治滢,樊竹青,董明华,等.云南星云湖酵母菌多样性及产类胡萝卜素的评价[J]. 微生物学通报, 2019,46(6):1309-1319.Li Z Y, Fan Z Q, Dong M H, et al. Diversity and evaluation of carotenoids-producing of yeasts isolated from Xingyun Lake[J]. Microbiology China, 2019,46(6):1309-1319.
[40]
方德新,吉芳英,许晓毅,等.高原高寒污水处理系统的微生物群落特征[J]. 中国环境科学, 2020,40(3):1081-1088.Fang D X, Ji F Y, Xu X Y, et al. Microbial community characteristics of wastewater treatment systems in high-altitude and cold regions[J]. China Environmental Science, 2020,40(3):1081-1088.
[41]
张瑞蕊,薛梅,李宁宁,等.天山一号冰川表面冰尘和底部沉积层中可培养酵母菌系统发育类群的分布及生态生理特征[J]. 微生物学报, 2019,59(2):334-348.Zhang R R, Xue M, Li N N, et al. Phylogeny and diversity of culturable yeasts in supraglacial cryoconite and subglacial sediments of the Glacier No.1in Tianshan Mountains[J]. Acta Microbiologica Sinica, 2019,59(2):334-348.
[42]
Virginia D G, Polona Z, Silvia B, et al. Cryptococcus species (Tremellales) from glacial biomes in the southern (Patagonia) and northern (Svalbard) hemispheres[J]. FEMS Microbiology Ecology, 2012,(2):523-539.
[43]
Białkowska A M, Szulczewska K M, Krysiak J, et al. Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples[J]. Polar Biology, 2017,40(9):1787-1803.
[44]
Sannino C, Tasselli G, Filippucci S, et al. Yeasts in nonpolar cold habitats[M]. Berlin:Springer, 2017.
[45]
de García V, Brizzio S, Libkind D, et al. Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina[J]. FEMS Microbiol Ecol, 2007,59(2):331-341.
[46]
Brandão L R, Libkind D, Vaz A B, et al. Yeasts from an oligotrophic lake in Patagonia (Argentina):diversity, distribution and synthesis of photoprotective compounds and extracellular enzymes[J]. FEMS Microbiol Ecol, 2011,76(1):1-13.
[47]
游庆龙,康世昌,李潮流,等.青藏高原纳木错气象要素变化特征[J]. 气象, 2007,33(3):54-60.Yong Q L, Kang S C, Li C L, et al. Variation features of meteorological elements at Namco station, Tibetan Plateau[J]. Meteorological Monthly, 2007,33(3):54-60.
[48]
王建辉,游庆国,申渝,等.短程反硝化-厌氧氨氧化耦合脱氮工艺影响因素与调控研究进展[J]. 环境化学, 2021,40(4):1216-1231.Wang J H, You Q G, Shen Y, et al. Research advances on influence factors and regulation of Partial denitrification and Anammox coupling denitrification process[J]. Environmental Chemistry, 2021,40(4):1216-1231.
[49]
杨文焕,杜璇,姚植,等.基于VAR模型的乌梁素海富营养化影响因子评价[J]. 环境科学与技术, 2021,44(2):25-32.Yang W H, Du X, Yao Z, et al. Evaluation of the Influencing Factors of Wuliangsuhai Eutrophication Based on VAR Model[J]. Environmental Science & Technology, 2021,44(2):25-32.
[50]
孙万儒.酵母菌[J]. 生物学通报, 2007,42(11):5-10.Sun W R. Yeast[J]. Bulletin of Biology, 2007,42(11):5-10.
[51]
Connell J H, Slatyer R O. Mechanisms of succession in natural communities and their role in community stability and organization[J]. American Naturalist, 1977,111(982):1119-1144.