模拟增温对木荷叶际抗生素抗性基因的影响

林小如; 李子歆; 孙安琪; 金圣圣; 徐超蝶; 陈仕东; 刘小飞; 沈菊培

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中国环境科学 ›› 2023, Vol. 43 ›› Issue (12) : 6689-6699.

新污染物

模拟增温对木荷叶际抗生素抗性基因的影响

林小如¹, 李子歆¹, 孙安琪¹, 金圣圣¹, 徐超蝶¹, 陈仕东^1,2, 刘小飞^1,2, 沈菊培¹

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Effect of stimulated warming on phyllosphere antibiotic resistance genes of Schima superba

LIN Xiao-ru¹, LI Zi-xin¹, SUN An-qi¹, JIN Sheng-sheng¹, XU Chao-die¹, CHEN Shi-dong^1,2, LIU Xiao-fei^1,2, SHEN Ju-pei¹

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摘要

为探究增温对叶际抗生素抗性基因(ARGs)分布的影响及其季节变化特征,本研究在夏季、秋季和冬季3个季节采集木荷叶片样品,设置对照(土壤不增温)与增温(土壤增温4℃)两个处理,采用高通量荧光定量PCR技术测定叶际ARGs的种类及相对丰度.结果表明:在所有叶际样品中共检测到255种ARGs和10种MGEs,其中多重耐药类是叶际优势抗生素抗性基因类型,相对丰度占总体的47.4%,而抗生素外排泵为主要抗性机制.方差分析和主坐标分析表明季节对叶际抗性基因的分布有显著影响,秋季和冬季叶际ARGs的相对丰度分别为(0.614±0.303)拷贝/16S rRNA和(0.589±0.218)拷贝/16S rRNA,高于夏季((0.291±0.101)拷贝/16S rRNA),且ARGs多样性同样表现为秋冬季高于夏季.研究检测到7种抗性基因(aadA2-03、acrA-02、emrD、matA/mel、tetB-01、tetM-01、tetL-02)的相对丰度在增温处理中显著下降(P<0.05),而仅有acrA-01基因相对丰度显著上升(P<0.05).冗余分析和结构方程模型显示,季节通过改变土壤理化性质间接影响叶际ARGs的分布,而增温对其没有产生显著影响.

Abstract

To understand the effect of warming on the distribution and seasonal dynamics of antibiotic resistance genes (ARGs) in the phyllosphere, we conducted a study involving collection of foliar samples from natural forest leaves during summer, autumn and winter from two treatments: control and warming (+4℃). The numbers and relative abundance of ARGs in the phyllosphere were determined by high-throughput quantitative PCR. Results showed that a total of 255 ARGs and 10 MGEs were detected in all phyllosphere samples, among which multidrug resistance was the dominant gene type in the phyllosphere, accounting for 47.4% of the total. The antibiotic efflux pump was the main resistance mechanism. ANOVA analysis and principal coordinate analysis showed that season significantly influenced the distribution of phyllosphere ARGs. The relative abundance of phyllosphere ARGs in autumn and winter was (0.614 ± 0.303)copies/16S rRNA and (0.589 ± 0.218)copies/16S rRNA, respectively, which was higher than that in summer ((0.291 ± 0.101)copies/16S rRNA), and the diversity of ARGs in autumn and winter was also higher than that in summer. We found that the relative abundance of seven ARGs(aadA2-03, acrA-02, emrD, matA/mel, tetB-01, tetM-01, tetL-02) decreased significantly (P<0.05), while the relative abundance of the acrA-01 gene increased significantly (P<0.05) in the warming treatment. Redundancy analysis and structural equation modeling showed that seasonality was the main factor driving the composition of phyllosphere ARGs via mediating soil physicochemical properties, while warming had no significant effect.

导出引用

林小如, 李子歆, 孙安琪, 金圣圣, 徐超蝶, 陈仕东, 刘小飞, 沈菊培. 模拟增温对木荷叶际抗生素抗性基因的影响[J]. 中国环境科学. 2023, 43(12): 6689-6699

LIN Xiao-ru, LI Zi-xin, SUN An-qi, JIN Sheng-sheng, XU Chao-die, CHEN Shi-dong, LIU Xiao-fei, SHEN Ju-pei. Effect of stimulated warming on phyllosphere antibiotic resistance genes of Schima superba[J]. China Environmental Science. 2023, 43(12): 6689-6699

中图分类号： X713

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