Effects of temperature and exogenous CaCO3 on the microbial degradation of ammonia nitrogen in waterworks sand filters
LIU Ming-hui1, DING Cheng-long1, ZHU Min1, LI Yi1, WU Jun-kang1, JING Zhao-qian1, YU Ran2
1. School of civil engineering, Nanjing Forestry University, Nanjing 210037, China; 2. School of Energy and Environment, Southeast University, Nanjing 210096, China
Abstract：In this paper, the ammonia degradation performance and variations of microbial community composition of the attached microbes in waterworks sand filters under different temperatures (10, 15, 25 and 37℃), ammonia concentrations (1 and 2.5mg-N/L) and exogenous addition of CaCO3 were investigated. The nitrifiers were successfully extracted from the sand filter in the waterworks, which included ammonia oxidizer, nitrite oxidizer and complete ammonia oxidizer as characterized by PCR- electrophoresis. The results of temperature influenced test showed that: the ammonia oxidation rate notably decreased by 48.1% and 47.3%, respectively under 1 and 2.5mg-N/L ammonia concentrations when the temperature decreased from 25℃ to 10℃. At 10℃, the system displayed high total nitrogen removal capacity (~48.1%), but the nitrite oxidation activity was significantly inhibited, and the increase of ammonia nitrogen concentrations exerted adverse effects on the nitrite oxidation. In contrast, the temperature as high as 37℃ caused the complete loss of the nitrification performance. Moreover, exogenous addition of CaCO3 significantly improved the ammonia oxidation rate by more than 90%. High-throughput sequencing results showed that a decrease in temperature (10℃ and 15℃) significantly inhibited the growth of Nitrosospira and Nitrosomonas (both relative abundance < 1%). When the temperature decreased to 10~15℃, Nitrospira outcompeted the other nitrifiers with the relative abundances increased by 63.3%-113.7%. Besides, the addition of CaCO3 caused a 2.5-fold increase in the relative abundance of Nitrospira, while the relative abundances of the other nitrifiers were all less than 1.0%. Therefore, it is inferred that the complete ammonia oxidation Nitrospira under cold condition had a high contribution to ammonia degradation.
刘明辉, 丁陈龙, 朱敏, 李仪, 吴俊康, 荆肇乾, 余冉. 温度及外源CaCO3对砂滤微生物氨氮降解的影响特性[J]. 中国环境科学, 2023, 43(9): 4896-4904.
LIU Ming-hui, DING Cheng-long, ZHU Min, LI Yi, WU Jun-kang, JING Zhao-qian, YU Ran. Effects of temperature and exogenous CaCO3 on the microbial degradation of ammonia nitrogen in waterworks sand filters. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4896-4904.
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