Preparation and immobilization mechanism of weathered coal-immobilized microbial cells
JIAO Zi-le1, LI Jian-hua1, CHEN Xiao-jing1,2, LU Jin-jing1, GAO Chun-hua1, XU Ming-gang1
Shanxi Province Key Laboratory of Soil Environment and Nutrient Resources, Shanxi Agricultural University, Taiyuan 030031, China; 2. Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China
Abstract：In this study, the modified weathered coal was used as the carrier to prepare for immobilized microbial cells by adsorption immobilization method. PBD (Plackett-Burman) and PBB (Box-Behnken) were used to optimize the immobilization process of microbial cells, so that the ability of this cells was maximized to passivate lead polluted water. A variety of characterization techniques were used to study the binding characteristics of microbial cells and carriers. These results showed that the quantity of microbial cells, immobilized temperature and pH were the key factors. When inoculation quantity was 7, the immobilized temperature was 31℃, and pH was 6.5, the unit Pb2+ adsorption capacity and removal rate of immobilized microbial cells could reach 313.83mg/g and 80.42%, which were 3.8times higher than free microbial cells. Compared with similar materials at home and abroad, it has a high level. Surface adsorption, pore filling, electrostatic attraction and covalent bonds (-OH, -NH2, -CONH2, -COOR) promote the immobilization of microorganisms on weathered coal jointly. This study can provide theoretical support for the preparation of immobilized microbial cells by weathered coal carriers.
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