基于短周期调控的SPNPR-A系统脱氮除磷研究

李冬; 王沁源; 毛中新; 齐赛月; 张杰

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中国环境科学 ›› 2024, Vol. 44 ›› Issue (8) : 4326-4335.

水污染与控制

基于短周期调控的SPNPR-A系统脱氮除磷研究

李冬¹, 王沁源¹, 毛中新¹, 齐赛月¹, 张杰^1,2

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Study on nitrogen and phosphorus removal in SPNPR-A system based on short-cycle regulation

LI Dong¹, WANG Qin-yuan¹, MAO Zhong-xin¹, QI Sai-yue¹, ZHANG Jie^1,2

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摘要

建立了短周期循环调控低浓度亚硝酸盐(NO₂^--N)的短程硝化同步除磷耦合厌氧氨氧化(SPNPR-A)系统,SPNPR系统以厌氧/好氧模式运行,通过短周期运行时长调控系统内NO₂^--N浓度用以淘洗亚硝酸盐氧化菌(NOB),同步富集氨氧化菌(AOB)和聚磷菌(PAOs),最终实现同步脱氮除磷功能.结果表明:SPNPR系统内NO₂^--N浓度维持在10mg/L左右时可有效抑制NOB活性.70d后SPNPR系统2个短周期的亚硝酸盐积累率(NAR)分别达到90.40%和88.93%,SPNPR-A系统总氮(TN)、总磷(TP)、有机物(COD)的平均去除率分别为87.45%、84.30%和94.26%.第60d时,比氨氧化速率(SAOR)和AOB的比氧利用速率(SOUR_AOB)分别为8.47mgN/(gMLVSS·h)和12.71mgO₂/(gMLVSS·h);比硝态氮生成速率(SNPR)和NOB的比氧利用速率(SOUR_NOB)分别为0.82mgN/(gMLVSS·h)和0.41mgO₂/(gMLVSS·h).高通量测序结果表明,第70d时SPNPR系统属水平上的AOB功能菌属Nitrosomonas(6.46%)和Nitrosospira(0.64%),丰度远高于NOB功能菌属Nitrospira(0.14%)和Nitrobacter(0.01%),同时聚磷菌属Candidatus_Accumulibacter(1.61%)和Tetrasphaera(0.89%)得到富集,使得SPNPR-A系统具备同步脱氮除磷性能.

Abstract

In this study, a simultaneous partial nitrification and phosphorus removal-anaerobic ammonia oxidation (SPNPR-A) system with short-cycle and low-concentration nitrite (NO₂^--N) was established. The SPNPR system operates in anaerobic/aerobic mode, and the NO₂^--N concentration in the system was limited for a brief duration to restrict nitrite oxidizing bacteria (NOB), simultaneously enriching ammonia oxidizing bacteria (AOB) and polyphosphate accumulating organisms (PAOs), and finally realizing synchronous nitrogen and phosphorus removal. The results showed that the NOB activity could be effectively inhibited by maintaining the NO₂^--N concentration in the SPNPR system at about 10mg/L for a short-cycle. After 70days, the nitrite accumulation rate (NAR) of the two short-cycles of SPNPR system reached 90.40% and 88.93%, respectively, and the average removal rates of total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (COD) in SPNPR-A system were 87.45%, 84.30% and 94.26%, respectively. At the 60th day, specific ammonia oxidation rate (SAOR) and specific oxygen utilization rate_AOB(SOUR_AOB) were 8.47mgN/(gMLVSS·h) and 12.71mgO₂/(gMLVSS·h), respectively. Specific nitrate production rate (SNPR) and specific oxygen utilization rate_NOB(SOUR_NOB) were 0.82mgN/(gMLVSS·h) and 0.41mgO₂/(gMLVSS·h), respectively. The results of high-throughput sequencing showed that, the abundance of AOB genera Nitrosomonas (6.46%) and Nitrosospira (0.64%) in SPNPR system was much higher than that of NOB genera Nitrospira (0.14%) and Nitrobacter (0.01%) on the 70th day, and at the same time, the phosphate accumulating organisms of Candidatus_Accumulibacter (1.61%) and Tetrasphaera (0.89%) were gradually enriched, so that the SPNPR-A system underscored the robust performance of synchronous nitrogen and phosphorus removal.

导出引用

李冬, 王沁源, 毛中新, 齐赛月, 张杰. 基于短周期调控的SPNPR-A系统脱氮除磷研究[J]. 中国环境科学. 2024, 44(8): 4326-4335

LI Dong, WANG Qin-yuan, MAO Zhong-xin, QI Sai-yue, ZHANG Jie. Study on nitrogen and phosphorus removal in SPNPR-A system based on short-cycle regulation[J]. China Environmental Science. 2024, 44(8): 4326-4335

中图分类号： X703

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