Start-up of the SNAD process for low ammonia-nitrogen sewage:Operational efficiency and ecological characteristics of microbe
WANG Zhao-zhao, WU Xin-juan, ZHU Shu-hao, AN Ning, MA Lei, LI Si-min
Hebei Technology Innovation Center of Water Pollution Control and Water Ecological Remediation, Handan Key Laboratory of Water Utilization Technology, College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China
Abstract：The Up-flow Microaerobic Sludge Blanket (UMSB-MBR) was employed to treat sewage with low ammonia-nitrogen and low C/N ratio, and the operational efficiency of the synchronous nitritation-anammox and denitrification (SNAD) process and the ecological characteristics of microbe were investigated. The results showed that, after start-ups of anammox, partial nitrification-anammox (PN/A) and SNAD processes, the nitrogen removal efficiency (NRE) at the end of each process was (80.85±0.81)%, (84.62±0.10)% and (90.01±0.23)%, respectively; and the COD removal efficiency (CRE) reached (85.04±0.18)% after SNAD process was successfully started up. Metagenomic sequencing results show that Nitrosomonas, the dominant bacteria of AerAOB, was enriched at the PN/A stage, and the relative abundance of ammoxidation functional genes (hao and amo) increased. The dominant genus of AnAOB was shifted from Candidatus_Kuenenia in Anammox process to Candidatus_Brocadia in SNAD process, and the functional genes (hzs and hdh) of anammox decreased first and then increased, indicating that AnAOB gradually adapted to low DO and low C/N ratio. The relative abundance of denitrifying bacteria (Ignavibacterium, unclassified-p-Chloroflexi) and denitrifying related genes (narG、narH、nirS、nirK) were at higher levels during the SNAD process, which proved their important roles in heterotrophic nitrogen removal process. The main nitrogen removal pathways in the system was verified to attribute to the simultaneous nitritation, anammox and denitrification reaction processes. Furthermore, microbial signal transduction and mass exchange and transport were demonstrated to stay at a higher level during the start-up of the system, and the cooperation ability within functional bacteria was significantly enhanced.
王朝朝, 武新娟, 朱书浩, 安宁, 马磊, 李思敏. 低氨氮污水SNAD工艺启动:运行效能与微生物生态学特性[J]. 中国环境科学, 2023, 43(5): 2254-2263.
WANG Zhao-zhao, WU Xin-juan, ZHU Shu-hao, AN Ning, MA Lei, LI Si-min. Start-up of the SNAD process for low ammonia-nitrogen sewage:Operational efficiency and ecological characteristics of microbe. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(5): 2254-2263.
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