Low temperature start-up characteristics and microbial population of BAFs for marine aquaculture wastewater treatment inoculated with two kinds of sludge
LU Li-quan1, QIU Li-ping1, LIU Pan-pan2, XIE Kang1, CHENG Hong-tao1, SHI Liang1, WANG Yan1
1. School of Civil Engineering and Architecture, University of Jinan, Jinan 250022, China;
2. Qilu Institute of Technology, Jinan 250200, China
In order to investigate the low temperature start-up characteristics of biological aerated filter (BAF) for simulated marine seawater aquaculture wastewater treatment,two zeolite media BAFs,which inoculated with marine sediment and activated sludge respectively,were operated under the conditions of hydraulic retention time 1h,water temperature 12~16℃,the ratio of gas to water 2:1(dissolved oxygen in 3~5mg/L),as well as the influent pH value of 7.38~8.23,permanganate index of 5.11~9.46mg/L,ammonia nitrogen 3.27~4.88mg/L.The results showed that the start-up time,average concentration of effluent ammonia nitrogen,removal rate of average ammonia nitrogen,average concentration of effluent permanganate index and removal rate of permanganate index of BAF inoculated with marine sediment were 39d,0.36mg/L,91%,4.96mg/L,31.9%,respectively,while the BAF inoculated with activated sludge were 35d,0.17mg/L,95.7%,4.63mg/L,36.5%,respectively.It was observed by DGGE and 16SrDNA gene sequencing analysis that the microbial population structure of two reactors had gone through a tremendous change.The Shannon index of original activated sludge,the marine sediment and the corresponding backwashing sludge the reactor stable operation after one month was 2.41,2.63,2.88 and 2.65,respectively.With the increase of running time,the deference of quantity and abundance microbial population in two BAFs decreased gradually,and the dominant population became more and more similar and consistent.Several salt-tolerant ammonia-oxidizing bacteria became dominant species,and the mainly population in stable operated BAF belonged to γ-proteobacteria class.
卢立泉, 邱立平, 刘盼盼, 谢康, 程洪涛, 石亮, 王琰. 接种BAF处理海水养殖废水低温启动及微生物特性[J]. 中国环境科学, 2017, 37(7): 2574-2582.
LU Li-quan, QIU Li-ping, LIU Pan-pan, XIE Kang, CHENG Hong-tao, SHI Liang, WANG Yan. Low temperature start-up characteristics and microbial population of BAFs for marine aquaculture wastewater treatment inoculated with two kinds of sludge. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2574-2582.
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