In order to explore the influence and the underlying mechanism of FA and PM2.5on hepatotoxicity,we conducted systematic research from three aspects,including toxicity of blood,hematopoietic organs (bone marrow,spleen) and myeloid progenitor.Male Blab/c mice were taken as experimental object,and were randomly divided into four groups:the control group;PM2.5-exposure group;FA-exposure group;PM2.5+FA-exposure group.Blood samples were firstly collected from mice caudal vein to conduct the complete blood count.Then the histopathological change of bone marrow and spleen (H&E staining) were observed.Furthermore,the oxidative damage (ROS,GSH),DNA damage (DPC,8-OH-dG) and apoptosis (caspase-3) in bone marrow,spleen and myeloid progenitor were detected.Results showed different degree of pathological changes in the bone marrow,spleen;compared with control group,oxidative damage,DNA damage,cell apoptosis level all demonstrated a rising trend in PM2.5,FA,PM2.5+FA group.In conclusion,an exposure combined formaldehyde and PM2.5 could cause hepatotoxicity in mice,with a possible mechanism from oxidative stress as well as its downstream DNA damage.
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