Behavior and ecotoxicity of single and mixed antibiotics in soils: Degradation and changes in microbial community
RUAN Lin-lin1,2, LIN Hui2, MA Jun-wei2, SUN Wan-chun2, YU Qiao-gang2, FU Jian-rong2
1. College of Life and Chemical Sciences, Zhejiang Normal University, Jinhua 321004, China; 2. Institute of Environmental Resources and Soil Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract：This work studied the short-term degradation of antibiotics in a purple clay and a silt soil after individual and combined application of sulfadimidine (SM2) and doxycycline (DOX);the responses of soil microbial community metabolism to different antibiotics perturbation were also compared;Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MicroRESPTM technology were used herein.Results indicated that the degradation rates of both SM2 and DOX on the 3rd and 7th day in the silt soil were higher than that in the purple clay.Compared with that in single antibiotic pollution systems,co-existence of DOX and SM2 in combined antibiotics pollution system inhibited the short-term degradation of both antibiotic types in the two soil types;this inhibition was stronger in the silt soil.Both the single and mixed antibiotics in this work enhanced the metabolisms of soil microbial community with antibiotic exposure concentration from 10mg/kg to 20mg/kg,whereas the impacts of antibiotics decreased as the exposure time of the antibiotic increased.In the purple clay,the mixed antibiotic treatment showed significantly higher metabolic diversity of soil microbial community than all the single antibiotic treatments;its metabolic activity and diversity of soil microbial community correlated positively and significantly with the DOX concentration.However,similar phenomena were not seen in the silt soil.Meanwhile,the effect of mixed antibiotic on soil microbial community metabolism was mainly due to the function of DOX herein.
阮琳琳, 林辉, 马军伟, 孙万春, 俞巧钢, 符建荣. 土壤中单一及复合抗生素的降解及微生物响应[J]. 中国环境科学, 2018, 38(3): 1081-1089.
RUAN Lin-lin, LIN Hui, MA Jun-wei, SUN Wan-chun, YU Qiao-gang, FU Jian-rong. Behavior and ecotoxicity of single and mixed antibiotics in soils: Degradation and changes in microbial community. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(3): 1081-1089.
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