The specific mixed bacteria were domesticated from the activated sludge of coking plant using double substrate domestication. The remediation capacity of the specific bacteria on petroleum hydrocarbon contaminated soil was studied under the optimum environmental conditions. High-throughput sequencing analysis revealed that Rhodanobacter sp. was the dominant bacterium, which accounted for 41%. The optimum degradation temperature and pH were 30~35℃ and 7~8, respectively. Tween 80can be utilized as carbon source to promote the growth of the specific bacteria and improve the degradation efficiency of petroleum hydrocarbons. Moreover, Tween 80showed greater promotion on petroleum hydrocarbon degradation in contaminated soil as compared to SDS. The greatest degradation efficiency of petroleum hydrocarbons occurred at Tween 80concentration of 5CMC (i.e. 375mg/L). Furthermore, the degradation efficiency of petroleum hydrocarbons after 80days reached 77% under the optimum environmental conditions, which evidenced the outstanding remediation performance of the specific bacteria on petroleum hydrocarbon contaminated soil.
王丽萍, 李丹, 许锐伟, 吴雪茜. 专性菌系对石油烃污染土壤的修复性能[J]. 中国环境科学, 2018, 38(4): 1417-1423.
WANG Li-ping, LI Dan, XU Rui-wei, WU Xue-qian. Remediation performance of constructed specific bacteria on petroleum hydrocarbon contaminated soil. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(4): 1417-1423.
Serial G A, Smith F L, Suidan M T, et al. Evaluation of Trickle Bed Biofilter Media for Toluene Removal[J]. Journal of the Air & Waste Management Association Volume, 1995,45(10):801-810.
Doong R A, Lei W G.Solubilization and mineralization of polycyclic aromatic hydrocarbons by Pseudomonas putida in the presence of surfactant[J]. Journal of Hazardous Materials, 2003,96(1):15-27.
[8]
Tian W, Yao J, Liu R, et al. Effect of natural and synthetic surfactants on crude oil biodegradation by indigenous strains[J]. Ecotoxicology and Environmental Safety, 2016,129:171-179.
Zhang Z, Zheng G, Lo I M C. Enhancement of nitrate-induced bioremediation in marine sediments contaminated with petroleum hydrocarbons by using microemulsions[J]. Environmental Science and Pollution Research, 2015,22(11):8296-8306.
[12]
Vila J, Lopez Z, Sabate J, et al. Identification of a novel metabolite in the degradation of pyrene by Mycobacterium sp. strain AP1:actions of the isolate on two-and three-ring polycyclic aromatic hydrocarbons[J]. Applied Environmental Microbiology, 2001,67(12):5497-5505.
[13]
Zhao D, Liu C, Liu L, et al. Selection of functional consortium for crude oil-contaminated soil remediation[J]. International Biodeterioration & Biodegradation, 2011,65(8):1244-1248.
[14]
Bailón L, Nikolausz M, Stner M K, et al. Removal of dichloromethane from waste gases in one-and two-liquid-phase stirred tank bioreactors and biotrickling filters[J]. Water Research, 2009,43(1):11-20.
[15]
Xiang T, Liu X, Zhang M, et al. Distribution of the indigenous microorganisms and mechanisms of their orientational activation in Daqing Oilfield[J]. Science in China Series D-earth Sciences, 2009,52(s1):128-134.
[16]
张晓娟.石油污染土壤中微生物对温度变化的响应研究[D]. 北京:中国地质大学(北京), 2016.
[17]
Al-Jawasim M, Yu K, Park J. Synergistic effect of crude oil plus dispersant on bacterial community in a louisiana salt marsh sediment[J]. Fems Microbiology Letters, 2015,362(17):144.