Comparison of microplastic extraction methods from organisms
WU Wen-nan1,2, GAO Jun-min1, SHEN Qian2, YAO Li-fen2, AN Li-hui2
1. Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China;
2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Microplastics (<5mm) have been detected in freshwater, marine and terrestrial organisms widely, however, no harmonized method is available which limited the scientific value for these existing investigation data. The present study aimed to compare four kinds of digestion solutions for microplastic isolation from fish and clam tissue, and then to evaluate the effects on 10 kinds of typical plastics of four kinds of digestion solutions by using a group of indicators, including morphological observation, quality change, fourier transform infrared spectroscopy and Raman spectroscopy characteristics. The results showed that the digestive efficiencies (%) were followed as the order:10%KOH > RIPA tissue lysate + Proteinase K > Proteinase K > 30%H2O2. In addition, PA particle quality increased after treatments, and PU particle colour slight changed after H2O2 treatment. Interestingly, the biodegradable plastic particles were soluble in 10% KOH completely. As expected, the infrared spectrum and Raman spectrum of plastic particles did not change before and after treatments, and the plastic particles could be identified based on their spectrum, except for the biodegradable plastic. As a conclusion, the treatment method of 10% KOH at 50℃ and 180r/min incubated for 6hours will be recommended as the preferred method for microplastic isolation from biological tissues.
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