Abstract：In this paper, a specific moving bed biofilm reactor (SMBBR), was engaged to treat the coking wastewater with its treatment performance being monitored continuously. The response relation between pollutants degradation and biofilm flora structure was illustrated by HS-GC/MS and Illumina high-throughput sequencing, and the influence of wastewater variables on microbial flora structure was analyzed by CCA. Results showed that the total phenol removal rate was 96.62percent when the system was operated stably for 50days, the thiocyanide and cyanide were completely degraded at 100days, phenol, thiocyanide and cyanide had toxic inhibition on NH4+-N degradation. HS-GC/MS results exhibited that after the aerobic treatment more than 80percent of the organic pollutants, including all phenols, partially the N, O heterocyclic compounds and long-chain alkanes were removed. The sequencing results displayed that there existed difference in the biofilm flora abundance and diversity based on the change of the reaction time. Proteobacteria, which promotes the degradation of phenol, had the highest relative abundance during the reaction. The dominant genera were norank_f_ODP1230B8.23, unclassified_o_Micrococcales, and norank_f_Anaerolineaceae. In addition, Thauera, Ottowia, unclassified_o_Rhizobiales and Thiobacillus proved to be the dominant degrading bacterium of phenol, SCN- and CN-in the system. CCA analysis exhibited that the pH value is the most positively correlated with Nitrospira, and effective pH control can contribute to the stable operation of the nitrification reaction. All these provide a theoretical basis for the biofilm treatment of the coking wastewater.
李卫平, 郝梦影, 敬双怡, 于玲红, 孙岩柏, 杨文焕. SMBBR处理焦化废水性能及菌群结构响应关系[J]. 中国环境科学, 2019, 39(8): 3332-3339.
LI Wei-ping, HAO Meng-ying, JING Shuang-yi, YU Ling-hong, SUN Yan-bai, YANG Wen-huan. Performance and flora structure response relation in the SMBBR treatment of the coking wastewater. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(8): 3332-3339.
Sharma N K, Philip L. Combined Biological and Photocatalytic Treatment of Real Coke Oven Wastewater[J]. Chemical Engineering Journal, 2016,295:20-28.
[2]
蒙小俊,李海波,盛宇星,等.焦化废水活性污泥PAH双加氧酶基因多样性分析[J]. 中国环境科学, 2017,37(1):367-372. Meng X J, Li H B, Sheng Y X, et al. Diversity of PAH dioxygenase genes of activated sludge from coking wastewater[J]. China Environmental Science, 2017,37(1):367-372.
[3]
Liu Y, Liu J, Zhang A, et al. Treatment effects and genotoxicity relevance of the toxic organic pollutants in semi-coking wastewater by combined treatment process[J]. Environmental Pollution, 2016, 220(A):13-19.
[4]
Zhang M, Tay J H, Qian Y, et al. Coke plant wastewater treatment by fixed biofilm system for COD and NH3-N removal[J]. Water Research, 1998,32(2):519-527.
[5]
Li H, Cao H, Li Y, et al. Innovative Biological Process for Treatment of Coking Wastewater[J]. Environmental Engineering Science, 2010, 27(4):313-322.
[6]
Wang Y Q, Lv Y L, Shan M J, et al. Engineering Application Research of Enhanced Microbiological Treatment for Coking Wastewater[J]. Applied Mechanics and Materials, 2011,108:257-261.
[7]
Sharma N K, Philip L. Treatment of Phenolics, Aromatic Hydrocarbons, and Cyanide-Bearing Wastewater in Individual and Combined Anaerobic, Aerobic, and Anoxic Bioreactors[J]. Applied Biochemistry and Biotechnology, 2014,175(1):300-322.
[8]
刘国新,吴海珍,孙胜利,等.市政污泥接种焦化废水好氧降解能力及微生物群落演替的响应分析[J]. 环境科学, 2017,38(9):3807-3816. Liu G X, Wu H Z, Sun S L, et al. Aerobic degradation and microbial community succession of coking wastewater with municipal sludge[J]. Environmental Science, 2017,38(9):3807-3816.
[9]
蒙小俊,李海波,曹宏斌,等.焦化废水活性污泥细菌菌群结构分析[J]. 环境科学, 2016,37(10):3923-3930. Meng X J, Li H B, Cao H B, et al. Bacterial community composition of activated sludge from coking wastewater[J]. Environmental Science, 2016,37(10):3923-3930.
[10]
Ma Q, Qu Y, Shen W, et al. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J]. Bioresource Technology, 2015,179:436-443.
[11]
Zhu S, Wu H, Wei C, et al. Erratum to:Contrasting microbial community composition and function perspective in sections of a full-scale coking wastewater treatment system[J]. Applied Microbiology and Biotechnology, 2016,100(2):949-960.
[12]
Zhao J L, Jiang Y X, Yan B, et al. Multispecies acute toxicity evaluation of wastewaters from different treatment stages in a coking wastewater-treatment plant[J]. Environmental Toxicology and Chemistry, 2014,33(9):1967-1975.
[13]
李卫平,李杰,朱浩君,等. SMBBR工艺不同填料处理生活污水[J]. 环境工程学报, 2015,9(9):4427-4433. Li W P, Li J, Zhu H J, et al. Treatment of domestic sewage by SMBBR process with different fillers[J]. Chinese Journal of Environmental Engineering, 2015,9(9):4427-4433.
[14]
Ma D H, Liu C, Zhu X B, et al. Acute toxicity and chemical evaluation of coking wastewater under biological and advanced physicochemical treatment processes[J]. Environmental Science and Pollution Research, 2016,23(18):18343-18352.
[15]
国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002:131-284. Editorial board of the State Environmental Protection Administration. Water and wastewater monitoring and analysis methods. The fouith edition[M]. Beijing:China Environmental Science Press, 2002:131-284.
[16]
张琲,解立平,秦梓雅,等.污泥水蒸气气化焦油化学组分的分布特征[J]. 中国环境科学, 2018,38(12):193-200. Zhang B, Xie L P, Qin Z Y, et al. Distribution characteristics of chemical components of the tar from sewage sludge steam gasification[J]. China Envirocnmental Sciene, 2018,38(12):193-200.
[17]
Joshi D R, Zhang Y, Gao Y, et al. Biotransformation of nitrogen-and sulfur-containing pollutants during coking wastewater treatment:Correspondence of performance to microbial community functional structure[J]. Water Research, 2017,121:338-348.
[18]
潘霞霞,李媛媛,黄会静,等.焦化废水中硫氰化物的生物降解及其与苯酚、氨氮的交互影响[J]. 化工学报, 2009,60(12):3089-3096. Pan X X, Li Y Y, Huang H J, et al. Biodegradation of thiocyanate and inhibitory interaction with phenol, ammonia in coking wastewater[J]. CIESC Journal, 2009,60(12):3089-3096.
[19]
Staib C, Lant P. Thiocyanate degradation during activated sludge treatment of coke-ovens wastewater[J]. Biochemical Engineering Journal, 2007,34(2):122-130.
[20]
Vazquez I, Rodriguez J, Maranon E, et al. Simultaneous removal of phenol, ammonium and thiocyanate from coke wastewater by aerobic biodegradation[J]. Journal of Hazardous Materials, 2006,137(3):1773-1780.
[21]
Joshi D R, Zhang Y, Tian Z, et al. Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater[J]. Applied Microbiology and Biotechnology, 2016,100(18):8191-8202.
[22]
莘明亮,隋秀斌,朱浩君,等. A/SMBBR工艺处理高氨氮戊二胺废水[J]. 水处理技术, 2017,43(6):114-117. Xin M L, Sui X B, Zhu H J, et al. Pilot-scale study on A/SMBBR process for high ammonia nitrogen DN5wastewater treatment[J]. Technology of Water Treatment, 2017,43(6):114-117.
[23]
梁东博,卞伟,王文啸,等.低温条件下好氧颗粒污泥培养及其脱氮性能研究[J]. 中国环境科学, 2019,39(2):188-194. Liang D B, Bian W, Wang W X, et al. Aerobic granular sludge formation and nutrients removal characteristics under low temperature[J]. China Envirocnmental Sciene, 2019,39(2):188-194.
[24]
Wang W, Ma W, Han H, et al. Thermophilic anaerobic digestion of Lurgi coal gasification wastewater in a UASB reactor[J]. Bioresource Technology, 2011,102(3):2441-2447.
[25]
Zhang T, Ding L, Ren H, et al. Ammonium nitrogen removal from coking wastewater by chemical precipitation recycle technology[J]. Water Research, 2009,43(20):5209-5215.
[26]
Manefield M, Griffiths R I, Leigh M B, et al. Functional and compositional comparison of two activated sludge communities remediating coking effluent[J]. Environmental microbiology, 2005, 7(5):715-722.
[27]
Ibarbalz F M, Figuerola E L, Erijman L. Industrial activated sludge exhibit unique bacterial community composition at high taxonomic ranks[J]. Water Research, 2013,47(11):3854-3864.
[28]
Kim Y M, Park H, Cho K H, et al. Long term assessment of factors affecting nitrifying bacteria communities and N-removal in a full-scale biological process treating high strength hazardous wastewater[J]. Bioresource Technology, 2013,134:180-189.
[29]
葛启隆,岳秀萍,王国英.一株苯酚降解菌的分离鉴定及响应面法优化其固定化[J]. 中国环境科学, 2014,34(2):518-525. Ge Q L, Yue X P, Wang G Y. Isolation and identification of a phenol-degrading strain and optimization for its immobilization using response surface methodology[J]. China Envirocnmental Sciene, 2014, 34(2):518-525.
[30]
Felfoldi T, Szekely A, Goral R, et al. Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent[J]. Bioresource Technology, 2010,101(10):3406-3414.
[31]
Yuste L, Corbella M E, Turiegano M J, et al. Characterization of bacterial strains able to grow on high molecular mass residues from crude oil processing[J]. Fems Microbiology Ecology, 2000,32(1):69-75.
[32]
Lay-Son M, Drakides C. New approach to optimize operational conditions for the biological treatment of a high-strength thiocyanate and ammonium waste:pH as key factor[J]. Water Research, 2008, 42(3):774-780.
[33]
甄建园,于德爽,王晓霞,等.低C/N(<3)条件下SNEDPR系统启动及其脱氮除磷特性研究[J]. 中国环境科学, 2018,38(8):2960-2967. Zhen J Y, Yu D S, Wang X X, et al. The nutrient removal characteristic of SNEDPR system during start-up and steady operation phases treating low C/N (<3)sewage[J]. China Envirocnmental Sciene, 2018, 38(8):2960-2967.
[34]
Klankeo P, Nopcharoenkul W, Pinyakong O. Two novel pyrene-degrading Diaphorobacter sp. and Pseudoxanthomonas sp. isolated from soil[J]. Journal of Bioscience & Bioengineering, 2009, 108(6):488-495.
