1. Department of Material Science and Engineering, Henan Institute of Technology, Xinxiang 453003, China; 2. Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Wuhan 430072, China
Abstract：In order to efficiently use wasted cyanobacteria and simultaneously yield vigorous phototrophic bacteria, the growth potential and transformation of carbon, nitrogen and phosphorus of Rhodopseudomonas palustris in cyanobacterial substrates were determined. A cyanobacterial concentration greater than 0.87g/L can stimulate the proliferation of R. palustris PUF1under the condition of 30℃, 2000lx. Though less of OD650 in cyanobacterial substrates (3g/L) than that of the ATYP medium at 108h, the concentration of bacteriochlorophyll a (Bchl a) was in similarity showing the more active synthesis of photopigments in cyanobacterial substrates. The addition of smaller molecular organic acids significantly regrew up the PUF1cells in late log phase. Specifically, at 252h and 444h, OD650 in cyanobacterial substrates was enhanced by 191.75% and 269.24%, while Bchl a was 206.68% and 276.17%, respectively. At 444h, OD650 and Bchl a in cyanobacterial substrates were separately 130.88% and 160.62% of the ATYP medium, indicating the superiority of natural cyanobacterial substrate to the ATYP medium for grown PUF1s, especially under a long incubation period. Evaluating the dried biomaterials and dissolved nutrients in cyanobacterial substrates, it indicated the unbalanced distribution of bioavailable carbon, nitrogen and phosphorus, namely surplus nitrogen, lacked carbon and limited phosphorus. It matters of improved bioavailable carbon in cyanobacterial biomasses for the enhanced growth of PUF1and nutrient sequestration.
王亚蕊,陈向超,付绪金,等.不同密度藻屑堆积下沉积物碳氮磷释放特征[J]. 湖泊科学, 2018,30(4):925-936. Wang Y R, Chen X C, Fu X J, et al. The releasing characteristics of carbon, nitrogen and phosphorus from sediment under the influence of different densities of algal detritus[J]. Journal of Lake Sciences, 2018, 30(4):925-936.
[2]
Chen W, Jia Y, Li E, et al. Soil-based treatments of mechanically collected cyanobacterial blooms from Lake Taihu:efficiencies and potential risks[J]. Environmental Science Technology, 2012,46:13370-13376.
[3]
胡万婷,唐千,孙伟,等.水体中蓝藻水华分解产甲烷动态过程研究[J]. 中国环境科学, 2017,37(2):702-710. Hu W T, Tang Q, Sun W, et al. Dissolved methane dynamics during the degradation of organic matter derived from cyanobacterial bloom[J]. China Environmental Science, 2017,37(2):702-710.
[4]
陆桂华,马倩.太湖水域"湖泛"及其成因研究[J]. 水科学进展, 2009,20(3):130-134. Lu G H, Ma Q. Analysis on the causes of forming black water cluster in Taihu Lake[J]. Advances in Water Science, 2009,20(3):130-134.
[5]
Sialve B, Bernet N, Bernard O. Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable[J]. Biotechnology Advances, 2009,27(4):409-416.
[6]
谭超,张乃明.利用新鲜蓝藻藻泥发酵制备苏云金芽胞杆菌杀虫剂[J]. 农药学学报, 2014,16(1):41-48. Tan C, Zhang N M. Fermentation of Bacillus thuringiensis based biopesticide using mud of blue-green algae[J]. Chinese Journal of Pesticide Science, 2014,16(1):41-48.
[7]
Tian Y, Wu X, Feng B, et al. Nutrient recovery from cyanobacteria biomasses using purple nonsulfur bacterium Rhodopseudomonas palustris[J]. Polish Journal of Environmental Studies, 2017,26:2767-2775.
[8]
Hunter C N, Daldal F, Thurnauer M C, et al. The purple phototrophic bacteria[M]. Berlin:Springer, 2008:1-12.
[9]
Chitapornpan S, Chiemchaisri C, Chiemchaisri W, et al. Organic carbon recovery and photosynthetic bacteria population in an anaerobic membrane photo-bioreactor treating food processing wastewater[J]. Bioresource Technology, 2013,141:65-74.
[10]
Yang A, Zhang G, Meng F, et al. Nitrogen metabolism in photosynthetic bacteria wastewater treatment:a novel nitrogen transformation pathway[J]. Bioresource Technology, 2019,294:122162-122169.
[11]
Liang C M, Hung C H, Hsu S C, et al. Purple nonsulfur bacteria diversity in activated sludge and its potential phosphorus-accumulating ability under different cultivation conditions[J]. Applied Microbial and Cell Physiology, 2010,86:709-719.
[12]
Peng C, Bryce C, Sundman A, et al. Cryptic cycling of complexes containing Fe(III) and organic matter by phototrophic Fe(II)-oxidizing bacteria[J]. Applied Environmental Microbiology, 2019,85(8).
[13]
Ghosh S, Dairkee U K, Chowdhury R, et al. Hydrogen from food processing wastes via photofermentation using purple non-sulfur bacteria (PNSB)-A review[J]. Energy Conversion and Management, 2016,141(1):299-314.
[14]
宗文明,于瑞嵩,樊美珍,等.一株利用生物柴油废水产氢的光合菌的筛选、鉴定[J]. 中国环境科学, 2009,29(10):107-108. Zong W, Yu R, Fan M, et al. Screening and identification of a photosynthetic bacterial strain for biohydrogen production from biodiesel wastewater[J]. China Environmental Science, 2009,29(10):107-108.
[15]
Garimella S, Kudle K R, Kasoju A, et al. Current status on single cell protein (SCP) production from photosynthetic purple non sulphur bacteria[J]. Journal of Chemical and Pharmaceutical Sciences, 2017, 10(2):915-922.
[16]
Ranaivoarisoa T O, Singh R, Rengasamy K, et al. Towards sustainable bioplastic production using the photoautotrophic bacterium Rhodopseudomonas palustris TIE-1[J]. Journal of Industrial Microbiology and Biotechnology, 2019,46(9/10):1401-1417.
[17]
Zhou Q, Li K, Jun X, et al. Role and functions of beneficial microorganisms in sustainable aquaculture[J]. Bioresource Technology, 2009,100:3780-3786.
[18]
Delamare-Deboutteville J, Batstone D J, Kawasaki M, et al. Mixed culture purple phototrophic bacteria is an effective fishmeal replacement in aquaculture[J]. Water Research X, 2019,4(1):100031.
[19]
李保珍,冯佳,谢树莲.光合细菌对铜绿微囊藻生长的抑制效应[J]. 生态环境学, 2009,18(5):1736-1740. Li B Z, Feng J, Xie S L. Inhibiting effect of photosynthetic bacteria on Microcystis aeruginosa[J]. Ecology and Environmental Sciences, 2009,18(5):1736-1740.
[20]
Sakarika M, Spanoghe J, Sui Y, et al. Purple non-sulphur bacteria and plant production:benefits for fertilization, stress resistance and the environment[J]. Microbial Biotechnology, 2019.
[21]
Kantachote D, Nunkaew T, Kantha T, et al. Biofertilizers from Rhodopseudomonas palustris strains to enhance rice yields and reduce methane emissions[J]. Applied Soil Ecology, 2016,100:154-161.
[22]
郭凌,张肇铭.利用球形红细菌降低小麦、油菜幼苗中镉积累的研究[J]. 山西大学学报(自然科学版), 2007,30(3):94-399. Guo L, Zhang Z M. Studies on decreasing the cadimium accumulation of wheat and cole seedlings by Rhodobacter sphaeroides[J]. Journal of Shanxi University (Nat. Sci. Ed.), 2007,30(3):394-399.
[23]
田莹莹,吴幸强,姬燕培,等.一株红假单胞菌的分离及生理特性研究[J]. 水生生物学报, 2020,44(2):429-527. Tian Y, Wu X, Ji Y, et al. Isolation and characterization of a Rhodopseudomonas sp. strain[J]. Acta Hydrobiologica Sinica, 2020, 44(2):429-527.
[24]
Ritchie R J, Runcie J W. Photosynthetic electron transport in an anoxygenic photosynthetic bacterium Afifella (Rhodopseudomonas) marina measured using PAM fluorometry[J]. Photochemistry and Photobiology, 2013,89(2):370-383.
[25]
国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002. State environmental protection administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002.
[26]
Hakobyan L, Gabrielyan L, Trchounian A. Yeast extract as an effective nitrogen source stimulating cell growth and enhancing hydrogen photoproduction by Rhodobacter sphaeroides strains from mineral springs[J]. International Journal of Hydrogen Energy, 2012,37(8):6519-6526.
[27]
Imai Y, Morita S, Arata Y. Proton correlation NMR studies of metabolism in Rhodopseudomonas palustris[J]. The Journal of Biochemistry, 1984,96:691-699.
[28]
Gabrielyan L, Trchounian A. Concentration dependent glycine effect on the photosynthetic growth and biohydrogen production by Rhodobacter sphaeroides from mineral springs[J]. Biomass Bioenergy, 2012,36:333-338.
[29]
Hiraishi A, Shi J L, Kitamura H. Effects of organic nutrient strength on the purple nonsulfur bacterial content and metabolic activity of photosynthetic sludge for wastewater treatment[J]. Journal of Fermentation and Bioengineering, 1989,68:269-276.
[30]
Okubo Y, Futamata H, Hiraishi A. Distribution and capacity for utilization of lower fatty acids of phototrophic purple nonsulfur bacteria in wastewater environments[J]. Microbes and Environments, 2005,20(3):135-143.
[31]
Hakobyan L, Gabrielyan L, Trchounian A. Proton motive force in Rhodobacter sphaeroides under anaerobic conditions in the dark[J]. Current Microbiology, 2011,62:415-419.
[32]
Fang H H, Zhu H G, Zhang T. Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides[J]. International Journal of Hydrogen Energy, 2006,31(15):2223-2230.
[33]
Androga D D, Özgür E, Eroglu I, et al. Amelioration of photofermentative hydrogen production from molasses dark fermenter effluent by zeolite-based removal of ammonium ion[J]. International Journal of Hydrogen Energy, 2012,37(21):16421-16429.
[34]
任洪艳,吕娴,阮文权.提高太湖蓝藻厌氧发酵产丁酸的预处理方法[J]. 食品与生物技术学报, 2011,30:100-105. Ren H, Lv X, Ruan W. Pretreatment methods to enhance the butyric acid production by anaerobic fermentation on Taihu Lake blue-green algae[J]. Journal of Food Science and Biotechnology, 2011,30:100-105.
[35]
刘刚,屠春宝,毕相东,等.碱法热处理对蓝藻厌氧发酵生物转化及微囊藻毒素降解效果的影响[J]. 农业资源与环境学报, 2016, 33:547-553. Liu G, Tu C, Bi X, et al. Effects of alkaline thermal pretreatment to cyanobacteria anaerobic digestion and microcystins degradation[J]. Journal of Agricultural Resources and Environment, 2016,33:547-553.
[36]
Li Q, Guo C, Liu C Z. Dynamic microwave-assisted alkali pretreatment of cornstalk to enhance hydrogen production via co-culture fermentation of Clostridium thermocellum and Clostridium thermosaccharolyticum[J]. Biomass and Bioenergy, 2014,64:220-229.
[37]
Yang G, Wang J. Pretreatment of grass waste using combined ionizing radiation-acid treatment for enhancing fermentative hydrogen production[J]. Bioresource Technology, 2018,255:7-15.
[38]
李小兰,周芸,陈志燕,等.针铁矿强化剩余污泥与烟草废弃物厌氧发酵的研究[J]. 中国环境科学, 2019,39(6):2475-2482. Li X, Zhou Y, Chen Z, et al. Enhanced anaerobic fermentation of waste activated sludge and tobacco waste using goethite[J]. China Environmental Science, 2019,39(6):2475-2482.
