In order to develop a quick start-up method for the anaerobic ammonia oxidation (Anammox) and Hydroxyapatite (HAP) (Anammox-HAP) granular sludge system, an Anammox attached film expanded bed reactor (AAFEB) was used, inoculated a small amount of Anammox sludge. The variation of sludge particle size and extracellular polymers (EPS) in the system were investigated by regulating influent substrate concentration and hydraulic residence time (HRT). Meanwhile, the nitrogen and phosphorus removal performance of the system was analyzed. Under the conditions of low up-flow rate of 0.213~1.066m/h and Ca/P=5.5molar ratio, the Anammox granular sludge system was started up successfully, with increasing influent nitrogen load. The removal efficiencies of total nitrogen and orthophosphate were (78.01±9.84)% and (63.83±9.89)%, respectively, and the total nitrogen volumetric loading reached 2.74kg/(m3·d). The average particle size of micro-granular sludge was 0.4mm in 150days. The morphology and element distribution of the sludge granules showed that they were Anammox-HAP granules. With the increase of granular sludge particle size, the proportion of PS in EPS was basically unchanged, while the proportion of PN increased from 54.43mg/g to 137.40mg/g, and PN/PS increased from 6.63to 7.71. There was a positive correlation between PN proportion in EPS and sludge particle size. It was PN that played a major role during sludge granulation.
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