The influences of petroleum pollution on the microbial population distribution in Northern Shaanxi Province of China
XU Yin-rui, WU Man-li, WANG Li, GAO Huan, LIU Ze-liang
Key Laboratory of Northwest Water Resources and Environment, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：Oil-contaminated soil and uncontaminated soil from oil well sites in ten oil-producing counties in Northern Shaanxi were collected. Chemical analysis and molecular biology techniques were used to determine the physicochemical properties and bacterial community structure of soil. Methods such as Pearson correlation analysis and redundancy analysis were used to study the impact of oil pollution on physicochemical properties, microbial population distribution and diversity of soil. Results showed that compared with uncontaminated soil, nitrate-nitrogen, total organic carbon, total carbon, total sulfur and carbon-nitrogen ratio of oil-contaminated soil increased, while the pH value decreased. The richness of soil bacterial community did not change significantly, but the evenness decreased significantly. The relative abundance of some dominant bacterial changed obviously after oil contamination in different regions. Changes in relative abundance of Proteobacteria and Chloroflexi were significantly positively correlated with petroleum hydrocarbon content, and changes in relative abundance of Candidatus Saccharibacteria were significantly negatively correlated with petroleum hydrocarbon content. The relative abundance of Pseudomonas, Nocardioides, Arthrobacter, Acinetobacter, Alkanindiges, Gaiella and GP4 increased significantly in Dingbian, Jingbian, Zichang, Suide, Wuqi, Ansai and Fuxian. The relative abundance of Ohtaekwangia, Pseudonocardia, Ramlibacter, and Sacchariacteria_genera_incertae_sedis decreased significantly in Dingbian, Jingbian, Zichang and Suide. Pseudomonas, Nocardioides, Arthrobacter and Alkanindiges were the main oilphilic bacteria in Northern Shaanxi Province. Physicochemical properties such as pH, moisture content, total petroleum hydrocarbon and total organic carbon were the key factors affecting the microbial population structure of oil-contaminated soil in Northern Shaanxi oilfield.
许殷瑞, 吴蔓莉, 王丽, 高欢, 刘泽梁. 陕北石油污染土壤微生物种群变化及影响因素[J]. 中国环境科学, 2021, 41(9): 4349-4359.
XU Yin-rui, WU Man-li, WANG Li, GAO Huan, LIU Ze-liang. The influences of petroleum pollution on the microbial population distribution in Northern Shaanxi Province of China. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4349-4359.
李晓楼.石油污染对土壤微生物群落多样性的影响[J]. 生物工程学报, 2017,33(6):968-975.Li X L. Influence of oil pollution on soil microbial community diversity[J]. Chinese Journal of Biotechnology, 2017,33(6):968-975.
[2]
Nwaichi E O. Evaluation of soil microbial communities as influenced by crude oil pollution[J]. African Journal of Biotechnology, 2015, 14(19):1632-1639.
[3]
Adesina G O, Adelasoye K A. Effect of crude oil pollution on heavy metal contents, microbial population in soil, and maize and cowpea growth[J]. Agricultural Sciences, 2014,5(1):43-50.
[4]
蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J]. 环境科学, 2018,39(7):3329-3338.Cai P P, Ning Z, He Z, et al. Microbial community distributions in soils of an oil exploitation site[J]. Environmental Science, 2018,39(7):3329-3338.
[5]
王金成,井明博,段春燕,等.石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J]. 水土保持通报, 2017,37(1):9-16.Wang J C, Jing M B, Duan C Y, et al. Impact of crude oil pollution on soil biological and abiological properties in eastern Gansu Province[J]. Bulletin of Soil and Water Conservation, 2017,37(1):9-16.
[6]
Gao Y, Miao C, Xia J, et al. Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial community structure[J]. Frontiers of Environmental Science & Engineering, 2012,6(2):213-223.
[7]
Nwaichi E O. Evaluation of soil microbial communities as influenced by crude oil pollution[J]. African Journal of Biotechnology, 2015,14(19):1632-1639.
[8]
刘五星,骆永明,滕应,等.石油污染土壤的生态风险评价和生物修复Ⅱ.石油污染土壤的理化性质和微生物生态变化研究[J]. 土壤学报, 2007,44(5):848-853.Liu W X, Luo Y M, Teng Y, et al. Co-risk assessment and bioremediation of petroleum contaminated soil II. Changes in physico-chemical properties and microbial ecology of petroleum contaminated soil[J]. Acta Pedologica Sinica, 2007,44(5):848-853.
[9]
王金成,井明博,肖朝霞,等.陇东黄土高原地区石油污染土壤微生物群落及其与环境因子的关系[J]. 水土保持通报, 2012,32(5):145-151.Wang J C, Jing M B, Xiao Z X, et al. Relationship between microbial-composition of petroleum-contaminated soil and its environmental factors in longdong region of loess plateau[J]. Bulletin of Soil and Water Conservation, 2012,32(5):145-151.
[10]
杨茜,吴蔓莉,聂麦茜,等.石油污染土壤的生物修复技术及微生物生态效应[J]. 环境科学, 2015,5:1856-1863.Yang Q, Wu M L, Nie M Q, et al. Effects and biological response on bioremediation of petroleum contaminated soil[J]. Environmental Science, 2015,5:1856-1863.
[11]
陈凯丽,吴蔓莉,叶茜琼,等.生物修复对石油污染土壤微生物活性的影响[J]. 农业环境科学学报, 2017,36(2):279-285.Chen K L, Wu M L, Ye X Q, et al. Impacts of bioremediation on microbial activities in petroleum contaminated soil[J]. Journal of Agro-Environment Science, 2017,36(2):279-285.
[12]
陈锐,赵玲侠,孙晓宇,等.陕北石油污染区土壤细菌群落结构解析[J]. 环境污染与防治, 2017,(11):1181-1185.Chen R, Zhao L X, Sun X Y, et al. Microbial community structure in petroleum polluted soil at north of Shaanxi[J]. Environmental Pollution & Control, 2017,(11):1181-1185.
[13]
刘培,梁继东,高伟,等.延安石油开采对周边黄土污染的调查分析[J]. 西安交通大学学报, 2011,(7):123-128.Liu P, Liang J D, Gao W, et al. Investigation of soil contamination caused by petroleum exploitation in Yan'an[J]. Journal of Xi'an Jiao Tong University, 2011,(7):123-128.
[14]
Risk-based cleanup levels for total petroleum hydrocarbons (TPH)[R]. Oklahoma:Department of Environmental Quality, 2010.
[15]
鲍士旦.土壤农化分析[M]. 北京:中国农业出版社, 2000.Bao S D. Soil and agricultural chemistry analysis[M]. Beijing:China Agriculture Press, 2000.
[16]
Wu M L, Dick W A, Li W, et al. Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum-contaminated soil[J]. International Biodeterioration & Biodegradation, 2016,107(3):158-164.
[17]
王如刚,王敏,牛晓伟,等.超声-索氏萃取-重量法测定土壤中总石油烃含量[J]. 分析化学, 2010,38(3):417-420.Wang R G, Wang M, Niu X W, et al. Determination of total petroleum hydrocarbons content in soil by ultrasonic-soxhlet extraction-gravimetric analysis[J]. Chinese Journal of Analytical Chemistry, 2010,38(3):417-420.
[18]
Zornoza R, Mataix-Solera J, Guerrero C, et al. Evaluation of soil quality using multiple lineal regression based on physical, chemical and biochemical properties[J]. Science of the Total Environment, 2007,378(1/2):233-237.
[19]
任芳菲.石油污染土壤的理化性质和微生物群落功能多样性研究[D]. 哈尔滨:东北林业大学, 2009.Ren F F. Studies on physico-chemical properties and microbial community functional diversity in petroleum contaminated soil[D]. Harbin:Northeast Forestry University, 2009.
[20]
Sugiura K, Ishihara M, Shimauchi T, et al. Physicochemical properties and biodegradability of crude oil[J]. Environmental Science and Technology, 1997,31(1):45-51.
[21]
贾建丽,刘莹,李广贺,等.油田区土壤石油污染特性及理化性质关系[J]. 化工学报, 2009,60(3):726-732.Jia J L, Liu Y, Li G H, et al. Contamination characteristics and its relationship with physicochemical properties of oil polluted soils in oilfields of China[J]. CIESC Journal, 2009,60(3):726-732.
[22]
李慧,陈冠雄,杨涛,等.沈抚灌区含油污水灌溉对稻田土壤微生物种群及土壤酶活性的影响[J]. 应用生态学报, 2005,16(7):1355-1359.Li H, Chen G X, Yang T, et al. Impacts of petroleum-containing wastewater irrigation on microbial population and enzyme activities in paddy soil of Shenfu irrigation area[J]. Chinese Journal of Applied Ecology, 2005,16(7):1355-1359.
[23]
Andrade M L, Covelo E F, Vega F A, et al. Effect of the prestige oil spill on salt marsh soils on the coast of Galicia (Northwestern Spain)[J]. Journal of Environmental Quality, 2004,33(6):2103-2110.
[24]
Leahy J G, Colwell R R. Microbial degradation of hydrocarbons in the environment[J]. Microbiological Reviews, 1990,54(3):305-315.
[25]
Dell'Anno A, Beolchini F, Gabellini M, et al. Bioremediation of petroleum hydrocarbons in anoxic marine sediments:Consequences on the speciation of heavy metals[J]. Marine Pollution Bulletin, 2009, 58(12):1808-1814.
[26]
Farkas M, Táncsics A, Kriszt B, et al. Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm[J]. International Journal of Systematic and Evolutionary Microbiology, 2015,65(Pt 1):274-279.
[27]
Zhao D F, Wu W L, Zhang Y B, et al. Study on isolation, identification of a petroleum hydrocarbon degrading bacterium bacillus fusiformis sp. and influence of environmental factors on degradation efficiency[J]. China Petroleum Processing & Petrochemical Technology, 2011, 13(4):74-82.
[28]
Xu J, Zhang Y, Hung T, et al. Growth characteristics of seven hydrocarbon-degrading active bacteria isolated from oil contaminated soil[J]. Nature Environment and Pollution Technology, 2013, 12(1):17-24.
[29]
王利利,董民,张璐,等.不同碳氮比有机肥对有机农业土壤微生物生物量的影响[J]. 中国生态农业学报, 2013,21(9):1073-1077.Wang L L, Dong M, Zhang L, et al. Effects of organic manures with different carbon-to-nitrogen ratios on soil microbial biomass of organic agriculture[J]. Chinese Journal of Eco-Agriculture, 2013, 21(9):1073-1077.
[30]
杨萌青,李立明,李川,等.石油污染土壤微生物群落结构与分布特性研究[J]. 环境科学, 2013,(2):789-794.Yang M Q, Li L M, Li C, et al. Microbial community structure and distribution characteristics in oil contaminated soil[J]. Environmental Science, 2013,(2):789-794.
[31]
Patel V, Sharma A, Lal R, et al. Response and resilience of soil microbial communities inhabiting in edible oil stress/contamination from industrial estates[J]. BMC Microbiology, 2016,16(1):50.
[32]
Chikere C B, Okoye A U, Okpokwasili G C, et al. Microbial community profiling of active oleophilic bacteria involved in bioreactor-based crude-oil polluted sediment treatment[J]. Journal of Applied & Environmental Microbiology, 2016,4(1):1-20.
[33]
侯宝卫,聂麦茜,聂红云,等.嗜油菌NY3对蜡质油污泥无害化处理[J]. 环境工程学报, 2015,9(1):341-347.Hou B W, Nie M Q, Nie H Y, et al. Harmless treatment of waxy oil sludge by Pseudomonas aeruginosa NY3[J]. Chinese Journal of Environmental Engineering, 2015,9(1):341-347.
[34]
Liu R, Zhang Y, Ding R, et al. Comparison of archaeal and bacterial community structures in heavily oil-contaminated and pristine soils[J]. Journal of Bioscience and Bioengineering, 2009,108(5):400-407.
[35]
Patel P A, Kothari V V, Kothari C R, et al. Draft genome sequence of petroleum hydrocarbon-degrading Pseudomonas aeruginosa strain PK6, isolated from the Saurashtra region of Gujarat, India[J]. Genome Announcments, 2014,2(1):1-2.
[36]
Whyte L G, Bourbonniére L, Greer C W. Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways[J]. Applied & Environmental Microbiology, 1997,63(9):3719-23.
[37]
Jiang Y, Qu Y, Xu P, et al. Genome sequence of a versatile aromatic hydrocarbon-degrading bacterium, Arthrobacter sp. W1[J]. Genome Announcements, 2015,3(2):1-2.
[38]
Gkorezis P, Bottos E M, Van Hamme J D, et al. Draft genome sequence of Arthrobacter sp. strain SPG23, a hydrocarbon-degrading and plant growth-promoting soil bacterium[J]. Genome Announcements, 2015,3(6):1-2.
[39]
Su G C, Zhou C Y, Cai H N, et al. Effectiveness of the cleaning performance of the bacterial strain Acinetobacter sp. SG06-02 in the petroleum hydrocarbon-polluted intestine of clams[J]. World Journal of Microbiology & Biotechnology, 2008,24(3):383-386.
[40]
Acer M, Kemal G, Poli A, et al. Acinetobacter mesopotamicus sp. nov. petroleum-degrading bacterium, isolated from petroleum-contaminated soil in Diyarbakir, in the southeast of Turkey[J]. Current Microbiology, 2020,77(2):3192-3200.
[41]
Chang W, Klemm S, Beaulieu C, et al. Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-arctic site[J]. Environmental Science & Technology, 2011,45(3):1061-1066.
[42]
Fuentes S, Barra B, Caporaso J G, et al. From rare to dominant:a fine-tuned soil bacterial bloom during petroleum hydrocarbon bioremediation[J]. Applied and Environmental Microbiology, 2015, 82(3):888-896.
