The influences of petroleum pollution on the microbial population distribution in Northern Shaanxi Province of China
XU Yin-rui, WU Man-li, WANG Li, GAO Huan, LIU Ze-liang
Key Laboratory of Northwest Water Resources and Environment, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：Oil-contaminated soil and uncontaminated soil from oil well sites in ten oil-producing counties in Northern Shaanxi were collected. Chemical analysis and molecular biology techniques were used to determine the physicochemical properties and bacterial community structure of soil. Methods such as Pearson correlation analysis and redundancy analysis were used to study the impact of oil pollution on physicochemical properties, microbial population distribution and diversity of soil. Results showed that compared with uncontaminated soil, nitrate-nitrogen, total organic carbon, total carbon, total sulfur and carbon-nitrogen ratio of oil-contaminated soil increased, while the pH value decreased. The richness of soil bacterial community did not change significantly, but the evenness decreased significantly. The relative abundance of some dominant bacterial changed obviously after oil contamination in different regions. Changes in relative abundance of Proteobacteria and Chloroflexi were significantly positively correlated with petroleum hydrocarbon content, and changes in relative abundance of Candidatus Saccharibacteria were significantly negatively correlated with petroleum hydrocarbon content. The relative abundance of Pseudomonas, Nocardioides, Arthrobacter, Acinetobacter, Alkanindiges, Gaiella and GP4 increased significantly in Dingbian, Jingbian, Zichang, Suide, Wuqi, Ansai and Fuxian. The relative abundance of Ohtaekwangia, Pseudonocardia, Ramlibacter, and Sacchariacteria_genera_incertae_sedis decreased significantly in Dingbian, Jingbian, Zichang and Suide. Pseudomonas, Nocardioides, Arthrobacter and Alkanindiges were the main oilphilic bacteria in Northern Shaanxi Province. Physicochemical properties such as pH, moisture content, total petroleum hydrocarbon and total organic carbon were the key factors affecting the microbial population structure of oil-contaminated soil in Northern Shaanxi oilfield.
许殷瑞, 吴蔓莉, 王丽, 高欢, 刘泽梁. 陕北石油污染土壤微生物种群变化及影响因素[J]. 中国环境科学, 2021, 41(9): 4349-4359.
XU Yin-rui, WU Man-li, WANG Li, GAO Huan, LIU Ze-liang. The influences of petroleum pollution on the microbial population distribution in Northern Shaanxi Province of China. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4349-4359.
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