Abstract：In this study, the microorganisms in aerobic activated sludge (Ae) and pure microbial Escherichia coli (E) were used as the main sources of soluble microbial products (SMP) to investigate the influence of photochemical and electric potential redox processes on the changes in functional groups, components and photochemical activity of SMP. The results showed that the SUVA254 and E2/E3 of Ae and E were 4.03/0.79 and 1.60/3.04, respectively, indicating that Ae has the higher molecular weight and aromaticity than E. Besides, illumination and electrochemistry significantly decreased the fluorescence intensity and changed the aromaticity and humification degree of SMP. And these two processes can increase the concentration of active oxidation species (3SMP*, 1O2 and ·OH) of SMP under illumination conditions. The [1O2]ss and [·OH]ss increased from 1.70mol/L and 3.57mol/L to 18.47mol/L and 438.25mol/L, respectively. In addition, the photodegradation kinetic of 17α-ethinylestradiol (EE2) mediated by SMP improved by almost 5 folds after being modified. This study improved the scientific understanding of the natural transformation processes of SMP in wastewater treatment plant effluent and the natural photodegradation of trace pollutants mediated by SMP.
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