Characterizing the estrogen content in cow feces based on gestation crate experiment
LI Yan1,2, PAN Jie1, LIANG Zi-wei2,3, HU Jia-wu2, LIU Jiang-yan1,2, HE De-chun2, LI Ting-zhen1
1. Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir, Chongqing Three Gorges University, Chongqing 404130, China; 2. South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangzhou 510530, China; 3. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Abstract:To explore characteristics of estrogen content in cow feces, fecal samples were collected from the gestation crates to quantify the contents of 4 natural estrogens (including estrone, 17α-estradiol, 17β-estradiol, estriol) and 2 synthetic estrogens (17α-ethinylestradiol, diethylstilbestrol) with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that the detection frequency (DF) of 6 estrogens ranged from 8.33% to 98.89%. The DF of detected natural estrogens was higher than 90%, except for estriol (8.33%); while the DF of synthetic estrogens were 52.22% and 64.44% for 17α-estradiol and diethylstilbestrol, respectively. The total content of 6 estrogens was in the range of 1.15~658.91μg/kg (dry weight, dw). The total estrogenic activity was equivalent to the content of 3.76~478.50μg/kg (dw). A significant difference in the total content (median) of 6 estrogens among different growth stages was observed in the order of: lactating cows (79.21μg/kg)>calves (47.20μg/kg)>heifers (25.72μg/kg). The content of estrogens in fecal samples demonstrate large seasonal variations: relatively higher in winter and spring than in summer and autumn andthe lowest concentration of 6 estrogens was observed in autumn. The total concentration of 6 estrogens in the feces collected from fecal stock dump ranged from 7.63 to 3921.92μg/kg which were significantly higher than that in fresh feces from gestation crate. Clearly, cow growth stage, sampling time (season) and sampling sites influenced the content of estrogens in cow feces, which should be fully considered in the investigation and risk assessment of estrogen emissions for cow breeding.
李艳, 潘杰, 梁紫薇, 胡嘉梧, 刘姜艳, 贺德春, 李廷真. 基于定位栏试验的奶牛粪便中雌激素含量特征[J]. 中国环境科学, 2022, 42(1): 119-126.
LI Yan, PAN Jie, LIANG Zi-wei, HU Jia-wu, LIU Jiang-yan, HE De-chun, LI Ting-zhen. Characterizing the estrogen content in cow feces based on gestation crate experiment. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 119-126.
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