不同阴离子钠盐对土壤Cd形态与微生物群落的影响

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摘要为了探明污染土壤在盐碱胁迫条件下镉（Cd）有效性与微生物群落的响应，采集污灌区Cd污染农田土壤，模拟北方土壤典型盐碱胁迫情景，设置不同阴离子钠盐配置处理，利用微生境培养实验，采用高通量测序技术，结合对土壤性质、土壤Cd形态等的测定.结果表明：施用钠盐可显著提高钠吸附比（SAR）、碱化度（ESP），降低有机碳（SOC）含量、阳离子交换量（CEC）；促进土壤中小粒径团聚体（<0.002mm）的形成，增加了Cd在小颗粒团聚体的质量负载；与对照相比，两种土壤中T1处理（主要阴离子为SO₄^2-、Cl^-）均显著（P<0.05）增加交换态Cd含量（27.06%和11.00%）.钠盐的添加降低了土壤中细菌的丰度和多样性，其中T1处理的微生物群落均匀度最低；盐碱胁迫处理改变了土壤细菌的关键类群，如增加了耐盐碱腈基降解菌科、葡萄球菌科、假单胞菌科和耐重金属芽孢杆菌科的菌群丰度，不同处理菌群结构差异与土壤阴离子组成有关，如相比而言，T1处理可增加变形菌门、芽单胞菌门、拟杆菌门的丰度.冗余分析结果表明土壤pH值、交换态Cd含量、SAR和ESP是影响细菌群落组成变化的关键环境因子，拟杆菌门、芽单胞菌门和变形菌门的丰度与pH值呈正相关，而酸杆菌门、绿弯菌门的丰度与交换态Cd含量、SAR呈现显著正相关.可见，盐碱胁迫增加了土壤Cd的有效性，显著改变了土壤微生物群落结构.

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	王立夫
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	李杉杉
	秦璐瑶
	孙晓艺
	雷小琴
	陈世宝
	王萌

关键词 ：污灌土壤, 镉有效性, 形态, 微生物群落

Abstract：In order to explore the impact of saline-alkaline stress on soil microbial community structures and Cd availability, different Cd-contaminated soils from wastewater-irrigated agricultural land were collected, the actual saline-alkali environment was simulated by adding the neutral and alkaline salts in different proportions. Soil microcosm incubations were conducted, Illumina high-throughput sequencing of 16S rRNA genes was used to estimate microbial community structures, and basic soil physiochemical properties and Cd content were determined. The results showed that salt addition positively affected sodium adsorption ratio (SAR), alkalinity (ESP), but reduced the content of soil organic carbon (SOC), cation exchange capacity (CEC). Increased soil salinity and alkalinity increased the proportion of small soil aggregates (<0.002mm), and promoted Cd translocation from large aggregates into clay/silt size fractions. Treatment T1 (mainly contained ions of SO₄^2- and Cl^-) enhanced exchangeable Cd content in both test soils by 27.06% and 11.00%. Soil saline-alkali stress decreased bacterial abundance and diversity, comparatively, soil microbial community in T1 showed the highest difference. Meanwhile, salt addition changed major soil bacterial taxa, for example, increased saline stress enriched saline-alkali tolerant taxa Nitriliruptoraceae, Staphylococcaceae, Pseudomonadaceae and heavy-metal resistant taxa Bacillaceae, microbial structure difference under different treatments probably depended the components of soil anions. For instance, comparatively, the abundance of Proteobacteria, Gemmatimonadetes and Bacteroidetes was significantly enriched in T1treatment. Redundancy analysis (RDA) of the abundant bacterial phyla and soil properties suggested that soil pH, exchangeable Cd content, SAR and ESP were the most influential environmental factors driving the changes in community composition, the abundances of Bacteroidetes, Gemmatimonadetes, and Proteobacteria were positively correlated with pH, and the abundances of Chloroflexi and Acidobacteria were strongly positively correlated with soil exchangeable Cd content, SAR. Therefore, saline-alkali stress increased soil Cd availability, significantly altered soil bacterial community.

Key words： wastewater-irrigated soil cadmium availability Cd fractions microbial community

收稿日期: 2021-02-14

PACS:	X53
	X172

基金资助:国家自然科学基金资助项目（41877387）；中央级公益性科研院所基本科研业务费专项（1610132021008）

通讯作者: 王萌,副研究员,wangmeng@caas.cn E-mail: wangmeng@caas.cn

作者简介: 王立夫(1997-),男,河北秦皇岛人,中国农业科学院农业资源与农业区划研究所硕士研究生,主要从事农田土壤重金属污染机制与修复相关研究.

引用本文:

王立夫, 赵淑雯, 李杉杉, 秦璐瑶, 孙晓艺, 雷小琴, 陈世宝, 王萌. 不同阴离子钠盐对土壤Cd形态与微生物群落的影响[J]. 中国环境科学, 2021, 41(9): 4221-4230. WANG Li-fu, ZHAO Shu-wen, LI Shan-shan, QIN Lu-yao, SUN Xiao-yi, LEI Xiao-qin, CHEN Shi-bao, WANG Meng. Effect of sodium salt with varied anions on Cd fractions and microbial community in soil. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4221-4230.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I9/4221

[1]	Balkhair K S, M A Ashraf. Field accumulation risks of heavy metals in soil and vegetable crop irrigated with sewage water in western region of Saudi Arabia[J]. Saudi Journal Of Biological Sciences, 2016,23(1):32-44.
[2]	Ma S C, Zhang H B, Ma S T, et al. Effects of mine wastewater irrigation on activities of soil enzymes and physiological properties, heavy metal uptake and grain yield in winter wheat[J]. Ecotoxicology and Environmental Safety, 2015,113:483-490.
[3]	何俊,王学东,陈世宝,等.典型污灌区土壤中Cd的形态、有效性及其影响因子[J]. 中国环境科学, 2016,36(10):3056-3063.He J, Wang X D,Chen S B, et al. The forms, bioavailability of Cd in soils of typical sewage irrigation fields in northern China and its control factors[J]. China Environmental Science, 2016,36(10):3056.
[4]	Kunhikrishnan A, Bolan N S,Muller K. The Influence of Wastewater Irrigation on the Transformation and Bioavailability of Heavy Metal (Loid) S in Soil[J]. Advances in Agronomy, 2012,115:215-297.
[5]	Raiesi F, Razmkhah M, Kiani S. Salinity stress accelerates the effect of cadmium toxicity on soil N dynamics and cycling:does joint effect of these stresses matter?. Ecotoxicology and Environmental Safety, 2018,153:160-167.
[6]	Zhao F J, Ma Y, Zhu Y G, et al. Soil contamination in China:Current status and mitigation strategies[J]. Environmental Science & Technology, 2015,49(2):750-759.
[7]	Delgado-Baquerizo M, Oliverio A M, Brewer T E, et al. A global atlas of the dominant bacteria found in soil[J]. Science, 2018,359(6373):320-325.
[8]	丁巧蓓,晁元卿,王诗忠,等.根际微生物群落多样性在重金属土壤修复中的研究[J]. 华南师范大学学报(自然科学版), 2016,48(2):1-12.Ding Q P, Chao Y Q, Wang S Z, et al. Research on function of rhizosphere microbial diversity in phytoremediation of heavy metal polluted soils[J]. Journal of South China Normal University (Natural Science Edition), 2016,48(2):1-12.
[9]	Liu L Z, Gong Z, Zhang Y, et al. Growth, cadmium uptake and accumulation of maize (Zea mays L.) under the effects of arbuscular mycorrhizal fungi[J]. Ecotoxicology, 2014,23(10):1979-1986.
[10]	Barcenas-Moreno G, E Baath, J Rousk. Functional implications of the pH-trait distribution of the microbial community in a re-inoculation experiment across a pH gradient[J]. Soil Biology & Biochemistry, 2016,93:69-78.
[11]	鲁如坤.土壤农业化学分析方法[M]. 北京:中国农业科技出版社, 1999.Lu R K. Agro-chemical analysis of soil[M]. Beijing:China Agricultural Science and Technology Press, 1999.
[12]	中华人民共和国国家卫生健康委员会.土壤质量铅、镉的测定石墨炉原子吸收分光光度法(GB/T 17141-1997)[S]. 北京:中华人民共和国国家卫生健康委员会, 1998:1-4.National Health Commission of PRC. Soil quality -determination of lead, cadmium-graphite furnace atomic absorption spectrophotometry (GB/T 17141-1997)[S]. Beijing:National Health Commission of PRC, 1998:1-4.
[13]	Manna M C, Zhang H B,Ma S T,et al. Long-term fertilization, manure and liming effects on soil organic matter and crop yields[J]. Soil & Tillage Research, 2007,94(2):397-409.
[14]	王萌,刘继芳,陈世宝,等.不同纳米修复剂对污染土壤中胡萝卜吸收、转运Cd的影响[J]. 环境工程学报, 2013,7(7):2738-2744.Wang M, Chen S B, Han Y, et al. Responses of soil aggregates and bacterial communities to soil-Pb immobilization induced by biofertilizer[J]. Chemosphere, 2019,220:828-836.
[15]	黄昌勇,土壤学[M]. 北京:中国农业出版社, 2000.Huang C Y. Soil science[M]. Beijing:China Agriculture Press, 2010.
	[14].
[16]	Sorokin D Y, van Pelt S, Tourova T P, et al. Nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of Nitriliruptoraceae fam. nov. and Nitriliruptorales ord. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2009,59(2):248-53.
[17]	Paul D. Kumbhare S V, Mhatre S S, et al. Exploration of microbial diversity and community structure of Lonar Lake:The only hypersaline meteorite crater lake within basalt rock[J]. Frontiers in Microbiology, 2016,6:1553.
[18]	Moreno M L, Piubeli F, Bonfa M R L, et al. Analysis and characterization of cultivable extremophilic hydrolytic bacterial community in heavy-metal-contaminated soils from the Atacama Desert and their biotechnological potentials[J]. Journal of Applied Microbiology, 2012.113(3):550-559.
[19]	Kuramshina Z M, Y V Smirnova, R M Khairullin. Increasing Triticum aestivum tolerance to cadmium stress through endophytic strains of Bacillus subtilis[J]. Russian Journal of Plant Physiology, 2016,63(5):636-644.
[20]	陈萍,何文寿.不同盐化土壤理化性质差异研究[J]. 农业科学研究, 2016,37(3):36-39.Chen P, He W T. Study on the difference of physical and chemical properties of different salinized soils[J]. Journal of Agricultural Sciences, 2016,37(3):36-39.
[21]	李杉杉.镉污染土壤高效钝化-植物阻控效果与机理研究[D]. 北京:中国地质大学(北京), 2019.Li S S. Effect and mechanism of Cd immobilization in Cd contaminated Farmland[D]. Beijing:China University of Geosciences (Beijing), 2019.
[22]	Wang T, Liu W, Xiong L, et al. Influence of pH, ionic strength and humic acid on competitive adsorption of Pb(II), Cd(II) and Cr(III) onto titanate nanotubes[J]. Chemical Engineering Journal, 2013:215-216.
[23]	Li S S, Wang M, Zhao Z Q, et al. Alleviation of cadmium phytotoxicity to wheat is associated with Cd re-distribution in soil aggregates as affected by amendments[J]. Rsc Advances, 2018, 8(31):17426-17434.
[24]	Malandrino M C, Swarup A, Wanjari R H, et al. Accumulation of heavy metals from contaminated soil to plants and evaluation of soil remediation by vermiculite[J]. Chemosphere, 2011,82(2):169-178.
[25]	Hale B, L Evans, R Lambert. Effects of cement or lime on Cd, Co, Cu, Ni, Pb, Sb and Zn mobility in field-contaminated and aged soils[J]. Journal of Hazardous Materials, 2012,199:119-127.
[26]	闫帅成,张克峰,刘雷,等.土壤中镉的形态及其影响因素研究进展[J]. 中国人口·资源与环境, 2016,26(S2):354-358.YAN S C, ZHANG K F, LIU L, et al., 2016. Research progress on the forms of cadmium in soil and its influencing factors[J]. China Population·Resources and Environment, 2016,26(S2):354-358.
[27]	颜世红.酸化土壤中镉化学形态特征与钝化研究[D]. 淮南:安徽理工大学, 2013.Yan S H. Study on the chemical form and passivation of cadmium in acidified soil[D]. Huainan:Anhui University of Science and Technology, 2013.
[28]	Wang Z W, Zheng X F, Xiao Y U, et al. Adsorption behaviors of Cd2+ on Fe2O3/MnO2 and the effects of coexisting ions under alkaline conditions[J]. Chinese Journal of Geochemistry, 2010,29(2):197-203.
[29]	王祖伟,弋良朋,等.碱性土壤盐化过程中阴离子对土壤中镉有效态和植物吸收镉的影响[J]. 生态学报, 2012,32(23):7512-7518.Wang Z W, Yi L P, Gao W Y, et al. Impact of inorganic anions on the cadmium effective fraction in soil and its phytoavailability during salinization in alkaline soils[J]. Acta Ecologica Sinica, 2012,32(23):7512-7518.
[30]	Tang X J, Li X, Liu X, et al. Effects of inorganic and organic amendments on the uptake of lead and trace elements by Brassica chinensis grown in an acidic red soil[J]. Chemosphere, 2015,119:177-183.
[31]	Wan D, Zhang N, Chen W, et al. Organic matter facilitates the binding of Pb to iron oxides in a subtropical contaminated soil[J]. Environmental Science and Pollution Research, 2018,25(32):32130-32139.
[32]	Burkhardt E M, Bischoff S, Akob D M, et al. Heavy metal tolerance of Fe(III)-reducing microbial communities in contaminated creek Bank Soils[J]. Applied and Environmental Microbiology, 2011,77(9):3132-3136.
[33]	Hamamura N, Olson S H, Ward D M, et al. Diversity and functional analysis of bacterial communities associated with natural hydrocarbon seeps in acidic soils at Rainbow Springs, Yellowstone National Park[J]. Applied and Environmental Microbiology, 2005,71(10):5943-5950.
[34]	Ledrich M L, Stemmler S, Laval-Gilly P, et al. Precipitation of silver-thiosulfate complex and immobilization of silver by Cupriavidus metallidurans CH34[J]. Biometals, 2005,18(6):643-650.
[35]	Alvarez A, Benimeli C S, Saez J M, et al. Bacterial bio-resources for remediation of hexachlorocyclohexane[J]. International Journal of Molecular Sciences, 2012,13(11):15086-106.
[36]	Wakelin S A, Nelson P N, Armour J D, et al. Bacterial community structure and denitrifier (nir-gene) abundance in soil water and groundwater beneath agricultural land in tropical North Queensland, Australia[J]. Soil Research, 2011,49(1):65-76.