Improving fermentative hydrogen production of anaerobic sludge by redox mediators
ZHANG Li-guo1,2, AI Bing-ling3, LI Jian-zheng4, BAN Qiao-ying1,2
1. College of Environment and Resource Sciences, Shanxi University, Taiyuan 030006, China; 2. Shanxi Laboratory for Yellow River, Taiyuan 030006, China; 3. Haikou Experimental Station, Chinese Academy Tropical Agricultural Sciences, Haikou 571101, China; 4. School of Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract：To improve the hydrogen production activity of anaerobic sludge, the effects of redox mediators (ROMs) on the hydrogen production efficiency of anaerobic sludge using glucose as substrate were investigated by batch culture. The microbial community structure was revealed by Illumina MiSeq sequencing. The results showed that the cumulative hydrogen production and maximum hydrogen production rate (Rmax) of the control were respectively 11.0mL and 0.28mL/h under conditions of the fermentative volume of 100mL and the initial glucose concentration of 500mg/L. When humic acid and anthraquinone-2-sulfonic acid (AQS) as ROMs were added to the system, the hydrogen production capacity of anaerobic sludge was significantly improved. The cumulative hydrogen production was higher than the control by 56.4% and 13.6%, respectively. While Rmax was increased by 53.6% and 10.7%, respectively. On the contrary, the hydrogen production capacity of anaerobic sludge was inhibited when graphene oxide (GO), hennaquinone and anthraquinone-2,6-disulfonic acid (AQDS) were used as ROMs. Illumina MiSeq sequencing revealed that the dominant microbial groups from each sample were different. Thermomarinilinea, Longilinea, Defluviimonas, Gimesia and Planctomicrobium were the dominant genera in control. The major genera were shifted to Thermomarinilinea, Longilinea and Ottowia in humic acid, AQS and hennaquinone fermentation systems. While Thermomarinilinea, Longilinea, Ottowia and Defluviimonas became the predominant genera in AQDS and GO fermentation systems. Redundancy analysis indicated that hydrogen production was positively correlated with Longilinea, Macellibacteroides, Trichococcus, Thioflavicoccus and Ottowia, whereas negatively correlated with Defluviimonas, Pirellula, Gimesia and Ignavibacterium.
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ZHANG Li-guo, AI Bing-ling, LI Jian-zheng, BAN Qiao-ying. Improving fermentative hydrogen production of anaerobic sludge by redox mediators. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(5): 2196-2202.
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