Investigation on the oynergistic mechanism of co-disposal of chromium-containing soil by digestate and ferrous sulfate
LI Rong-qiang1, WANG Shuai2, ZHANG Hai-xiu3, SHA Fu-jian4, ZHANG Da-lei1, ZHAO Jian-wei1, SUN Ying-jie1
1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Shandong Qingdao 266033, China; 2. Qingdao Municipal Bureau of Ecology and Enviroment, Chengyang Branch, Shandong Qingdao 266100, China; 3. Jierui Environmental Protection Technology Co., Ltd., Shandong Yantai 264003, China; 4. The Second Hydrogeology and Engineering Geological Team of Shandong Provincial Geological Survey Bureau (Shandong Lubei Geological Engineering Investigation Institute), Shandong Dezhou 253072, China
Abstract：Digestate and ferrous sulfate were combined to co dispose the chromium-containing soil, the synergistic effect of co-disposal was verified after the preliminarily optimizing of disposal parameters, and the synergistic mechanism was revealed by XPS analysis and microbial community analysis. Co-disposal of digestate and ferrous sulfate could reduce the content of Cr (VI) in soil to undetected (the detection limit was 0.2mg/kg), which was better than that with ferrous sulfate treatment, and the reduction rate of co-dispose was higher than that of digestate alone. The analysis of microbial community structure showed that the abundance and diversity of bacterial community in co-dispose group was higher than that in digestate alone, and the relative abundance of chromium reducing bacteria, iron reducing bacteria and sulfate reducing bacteria was significantly increased in the co-dispose soil. XPS analysis showed that Fe(Ⅱ), sulfites and sulfides were present in the soil after co-dispose. Combined with the results of microbial community analysis, the process of microbial catalytic reduction of Cr (VI) in digestate by iron and sulfur was confirmed. This study provides a new idea for the treatment of low carbon high concentration Cr (VI) contaminated soil.
荣强, 王帅, 张海秀, 沙福建, 张大磊, 赵建伟, 孙英杰. 沼渣与硫酸亚铁共处置含铬土壤的协同机制研究[J]. 中国环境科学, 2022, 42(5): 2229-2237.
LI Rong-qiang, WANG Shuai, ZHANG Hai-xiu, SHA Fu-jian, ZHANG Da-lei, ZHAO Jian-wei, SUN Ying-jie. Investigation on the oynergistic mechanism of co-disposal of chromium-containing soil by digestate and ferrous sulfate. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2229-2237.
肖文丹,叶雪珠,孙彩霞,等.铬耐性菌对土壤中六价铬的还原作用 [J]. 中国环境科学, 2017,37(3):1120-1129. Xiao W D, Ye X Z, Sun C X, et al. The effect of chromium-resistant bacteria on reduction of hexavalent chromium in soils [J]. China Environmental Science, 2017,37(3):1120-1129.
[2]
Zhang D L, Zhang M Y, Zhang C H, et al. Pyrolysis treatment of chromite ore processing residue by biomass: cellulose pyrolysis and Cr(VI) reduction behavior [J]. Environmental Science & Technology, 2016,50(6):3111-3118.
[3]
张大磊,李公伟,李卫华,等.聚乙烯塑料/铬渣共热解还原Cr(Ⅵ)的实验研究 [J]. 中国环境科学, 2017,37(5):1852-1857. Zhang D L, Li G W, Li W H, et al. Experimental study on reduction of Cr (VI) by co-pyrolysis of polyethylene/chromite ore processing residue [J]. China Environmental Science, 2017,37(5):1852-1857.
[4]
Song Z L, Fang L C, Wang J, et al. Use of biogas solid residue from anaerobic digestion as an effective amendment to remediate Cr(VI)- contaminated soils [J]. Environmental Science and Pollution Research International, 2019,26(13):13041-13053.
[5]
Wang D H, Li G H, Qin S Q, et al. Remediation of Cr(VI)- contaminated soil using combined chemical leaching and reduction techniques based on hexavalent chromium speciation [J]. Ecotoxicology and Environmental Safety, 2021,208:111734.
[6]
Li L, Shang X F, Sun X j, et al. Bioremediation potential of hexavalent chromium by a novel bacterium Stenotrophomonas acidaminiphila 4-1 [J]. Environmental Technology & Innovation, 2021,22:101409.
[7]
Cheng Z H, Xiong J R, Min D, et al. Promoting bidirectional extracellular electron transfer of shewanella oneidensis MR-1for hexavalent chromium reduction via elevating intracellular cAMP level [J]. Biotechnology and Bioengineering, 2020,117(5):1294-1303.
[8]
杨明超,余光辉,徐阳春,等.沼渣堆肥中Cu和Zn形态与有机官能团特征 [J]. 中国环境科学, 2019,39(4):1639-1644. Yang M C, Yu G H, Xu Y C, et al. Characterization of Cu and Zn species as well as the distribution of functional groups during composting of biogas residues and rice straw [J]. China Environmental Science, 2019,39(4):1639-1644.
[9]
孔 鑫,刘建国,刘意立,等.基于高压挤压预处理的生活垃圾干湿分离处理工艺不同场景综合效益分析 [J]. 环境科学学报, 2020,40(9): 3445-3452. Kong X, Liu J G, Liu Y L, et al. Analysis of comprehensive environmental benefits in different municipal solid waste treatment scenarios based on dry-wet fraction separation through high-pressure extrusion pretreatment [J]. Acta Scientiae Circumstantiae, 2020.40(9): 3445-3452.
[10]
Meng X Y, Yan J, Zuo B, et al. Full-scale of composting process of biogas residues from corn stover anaerobic digestion: Physical- chemical, biology parameters and maturity indexes during whole process [J]. Bioresource Technology, 2020,302:122742.
[11]
封 琳.厌氧消化污泥解毒铬污染土壤实验研究 [D]. 青岛:青岛理工大学, 2013. Feng L. Detoxification of chromium-contaminated soil with anaerobic digestion sludge [D]. Qingdao: Qingdao University of Technology, 2013.
[12]
Zhang D L, Zhao Y H, Sun Y F, et al. In-situ detoxification method for heavily contaminated site by hexavalent chromium, chromium soil remediation microbial agent and preparation method thereof: US, 10596606 [P]. 2019.
[13]
Zhang D L, Zhao Y H, Chen L, et al. Biogas residue-based in situ and ectopic coupled detoxification method for hexavalent chromium- containing sites and soil remediation agent: US. 10,688,546 [P]. 2020.
[14]
HJ1082-2019 土壤和沉积物 六价铬的测定 碱溶液提取-火焰原子吸收分光光度法 [S]. HJ1082-2019 Soil and sediment-Determination of Cr(VI)-Alkaline digestion/ flame atomic absorption spectrometry [S].
李培中,吕晓健,王海见,等.某电镀厂六价铬污染土壤还原稳定化试剂筛选与过程监测 [J]. 环境科学, 2017,38(1):368-373. Li P Z, Lv X J, Wang H J, et al. Screening of the reduction /stabilization reagent for Cr(Ⅵ) contaminated soil in a planting site and reaction process monitoring [J]. Environmental Science, 2017, 38(1):368-373.
[22]
Chen J, Tian Y Q. Hexavalent chromium reducing bacteria: mechanism of reduction and characteristics [J]. Environmental Science and Pollution Research International, 2021,28:20981-20997.
[23]
Tan H, Wang C, Li H, et al. Remediation of hexavalent chromium contaminated soil by nano-FeS coated humic acid complex in combination with Cr-resistant microflora [J]. Chemosphere, 2020,242: 125251.
[24]
Liu Y G, Xu W H, Zeng G M, et al. Cr (VI) reduction by Bacillus sp. isolated from chromium landfill [J]. Process Biochemistry, 2006,41(9): 1981-1986.
[25]
郑家传,张建荣,刘希雯,等.污染场地六价铬的还原和微生物稳定化研究 [J]. 环境科学, 2014,35(10):3882-3887. Zheng J C, Zhang J R, Liu X W, et al. Bioremediation of chromium (Ⅵ) contaminated site by reduction and microbial stabilization of chromium [J]. Environmental Science, 2014,35(10):3882-3887.
[26]
Zhang B G, Liu J, Sheng Y Z, et al. Disentangling microbial syntrophic mechanisms for hexavalent chromium reduction in autotrophic biosystems [J]. Environmental Science & Technology, 2021,55(9): 6340-6351.
[27]
Essahale A, Malki M, Marín I, et al. Hexavalent chromium reduction and accumulation by acinetobacter AB1isolated from fez tanneries in morocco [J]. Indian Journal of Microbiology, 2012,52(1):48-53.
[28]
Li M H, Gao X Y, Li C, et al. Isolation and identification of chromium reducing bacillus cereus species from chromium-contaminated soil for the biological detoxification of chromium [J]. International Journal of Environmental Research and Public Health, 2020,17(6):2118.
[29]
Olga P, Ikkert, Anna L, et al. Characterization of precipitates formed by H2S-producing, Cu-resistant Firmicute isolates of Tissierella from human gut and Desulfosporosinus from mine waste [J]. Antonie van Leeuwenhoek, 2013,103(6):1221-1234.
[30]
Slobodkin A I, Tourova T P, N. Kostrikina A, et al. Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe (III)-reducing bacterium of the order Clostridiales [J]. International Journal of Systematic and Evolutionary Microbiology, 2006,56(2): 369-372.
[31]
Tang X, Huang Y, Li Y, et al. Study on detoxification and removal mechanisms of hexavalent chromium by microorganisms [J]. Ecotoxicology and Environmental Safety, 2021,208:111699.
