Abstract:Penicillium pinophilum was used as an example in this study and headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to collect and analyze microbial volatile organic compounds (MVOCs). The effects of the growth time, the temperature, the light and the oxygen content on the characteristics of MVOCs were investigated, and each contribution to indoor VOCs was discussed. A total of 6categories and 14 MVOCs were detected, including higher-concentration MVOCs such as ethanol, acetone, acetic acid, ethyl acetate, isoamyl alcohol and anisole, and lower-concentration MVOCs such as isobutanol, 2-methylbutanol, 2-Furanmethanol, toluene, m-xylene, 1-octene-3-ol, 3-octanol and limonene. The most obvious effects on MVOCs emission were the oxygen content and the growth time, which mainly affected the competitive metabolism of ethanol and acetone. The amounts of acetic acid and ethyl acetate were higher when the oxygen content was lower. There were considerable discrepancies of the types and the quantities of metabolites of Penicillium pinophilum along the growth time. The total amount of MVOCs reached maximum on the 4th~6th day of inoculation. Except acetic acid and limonene, other substances were obviously affected by the growth time. The maximum amount of MVOCs was produced at 25℃, and the minimum was at 35℃. The components of MVOCs were influenced by temperature. The light intensity had no obvious effect on the metabolism of Penicilliumpinophilum. Under three experimental temperatures with certain oxygen content and light, the emission intensity of total MVOCs was between 5031and 7650ng/(m2×d), and the contribution of MVOCs concentrations to indoor air ranged from 0.0256 to 444.0380ng/m3.
温胜超, 刘兆荣. 室内环境嗜松青霉的MVOCs释放特征及影响因素[J]. 中国环境科学, 2022, 42(11): 5055-5062.
WEN Sheng-chao, LIU Zhao-rong. Characteristics and influencing factors of microbial volatile organic compounds from a common indoor mold. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5055-5062.
Lonnblad P, Kokotti H, Kujanpaa L, et al. Occurrence of microbes in non-ventilated outer walls and health effects[C]//Proceedings of the 10th International Conference on Indoor Air Quality and Climate, 2005:4-9.
[2]
Ruth J H. Odor thresholds and irritation levels of several chemical substances:A review[J]. American Industrial Hygiene Association journal, 1986,47(3):142-151.
[3]
Morath S U, Hung R, Bennett J W. Fungal volatile organic compounds:A review with emphasis on their biotechnological potential[J]. Fungal Biology Reviews, 2012,26(2/3):73-83.
[4]
Fang Z, Tang Q, Gong C, et al. Profile and distribution characteristics of culturable airborne fungi in residential homes with children in Beijing, China[J]. Indoor and Built Environment, 2015,26(9):1232-1242.
[5]
Odebode A, Adekunle A, Stajich J, et al. Airborne fungi spores distribution in various locations in Lagos, Nigeria[J]. Environmental Monitoring and Assessment, 2020,192(2):87-101.
[6]
Sautour M, Sixt N, Dalle F, et al. Profiles and seasonal distribution of airborne fungi in indoor and outdoor environments at a French hospital[J]. Science of The Total Environment, 2009,407(12):3766-3771.
[7]
Korpi A, Jarnberg J, Pasanen A L. Microbial volatile organic compounds[J]. Critical Reviews in Toxicology, 2009,39(2):139-193.
[8]
Mølhave L, Liu Z, Jørgensen A H, et al. Sensory and physiological effects on humans of combined exposures to air temperatures and volatile organic compounds[J]. Indoor Air, 1993,3(3):155-169.
[9]
Mølhave L. Volatile organic compounds and sick building syndrome[M]. Environmental Toxicants Hoboken, NJ, USA:John Wiley & Sons, Inc, 2009:241-256.
[10]
Araki A, Kawai T, Eitaki Y, et al. Relationship between selected indoor volatile organic compounds, so-called microbial VOC, and the prevalence of mucous membrane symptoms in single family homes[J]. Science of The Total Environment, 2010,408(10):2208-2215.
[11]
Mendell M J, Mirer A G. Dampness, mould, and health-a review of epidemiologic evidence for the upcoming WHO guidelines for indoor air quality[J]. Epidemiology, 2008,19(6):S136-S137.
[12]
Arthur R. Damp indoor spaces and health[M]. Oxford:Oxford University Press, 2005:234-234.
[13]
Choi H, Schmidbauer N, Bornehag C G. Volatile organic compounds of possible microbial origin and their risks on childhood asthma and allergies within damp homes[J]. Environment International, 2017,98:143-151.
[14]
Adan C G, Samson R A. Fundamentals of mold growth in indoor environments and strategies for healthy living[C]//Wageningen, Wageningen Academic Publishers, 2011:277-302.
[15]
Misztal P K, Lymperopoulou D S, Adams R I, et al. Emission factors of microbial volatile organic compounds from environmental bacteria and fungi[J]. Environmental Science & Technology, 2018,52(15):8272-8282.
[16]
Stefano S, Nicoletti R, Zambardino S, et al. Structure elucidation of a novel funicone-like compound produced by Penicillium pinophilum[J]. Natural Product Letters, 2002,16(3):207-211.
[17]
Qing S W. Identification and Biocontrol of Latent Pathogenic Fungi in Blueberry Fruits[J]. Northern Horticulture, 2017,18:41-48.
[18]
Volke Sepulveda T, Saucedo Castaneda G, Gutierrez Rojas M, et al. Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger[J]. Journal of Applied Polymer Science, 2002,83(2):305-314.
[19]
Jeya M, Joo A R, Lee K M, et al. Characterization of endo-beta-1, 4-glucanase from a novel strain of Penicillium pinophilum KMJ601[J]. Applied Microbiology and Biotechnology, 2010,85(4):1005-1014.
[20]
Zhao G, Yin G, Inamdar A, et al. Volatile organic compounds emitted by filamentous fungi isolated from flooded homes after hurricane Sandy show toxicity in a Drosophila bioassay[J]. Indoor Air, 2017, 27(3):518-528.
[21]
Nilsson T, Larsen T, Montanarella L, et al. Application of head-space solid-phase microextraction for the analysis of volatile metabolites emitted by Penicillium species[J]. Journal of Microbiological Methods, 1996,25(3):245-255.
[22]
Wilkins K, Larsen K, Simkus M. Volatile metabolites from mold growth on building materials and synthetic media[J]. Chemosphere, 2000,41(3):437-446.
[23]
Boots A W, Smolinska A, van Berkel J, et al. Identification of microorganisms based on headspace analysis of volatile organic compounds by gas chromatography-mass spectrometry[J]. Journal of Breath Research, 2014,8(2):027106.
[24]
Oro L, Feliziani E, Ciani M, et al. Volatile organic compounds from Wickerhamomyces anomalus, Metschnikowia pulcherrima and Saccharomyces cerevisiae inhibit growth of decay causing fungi and control postharvest diseases of strawberries[J]. International Journal of Food Microbiology, 2018,265:18-22.
[25]
Pasanen A L, Lappalainen S, Pasanen P. Volatile organic metabolites associated with some toxic fungi and their mycotoxins[J]. Analyst, 1996,121(12):1949-1953.
[26]
Cheng Z, Li M, Marriott P J, et al. Chemometric analysis of the volatile compounds generated by Aspergillus carbonarius strains isolated from grapes and dried vine fruits[J]. Toxins, 2018,10(2):71-88.
[27]
Pitt R E. a descriptive model of mold growth and aflatoxin formation as affected by environmental-conditions[J]. Journal of Food Protection, 1993,56(2):139-146.
[28]
Viitanen H, Ojanen T. Improved model to predict mold growth in building materials[J]. Ashrae, 2007:1-8.
[29]
Choi K S, Park Y B, Kim J, et al. Characteristics of photosynthesis and leaf growth of Peucedanum japonicum by leaf mold and shading level in forest farming[J]. Korean Journal of Medicinal Crop Science, 2015,23(1):43-48.
[30]
Yamaga I, Takahashi T, Ishii K, et al. Suppression of blue mold symptom development in satsuma mandarin fruits treated by low-intensity blue LED irradiation[J]. Food Science and Technology Research, 2015,21(3):347-351.
[31]
Kalalian C, Abis L, Depoorter A, et al. Influence of indoor chemistry on the emission of mVOCs from Aspergillus niger molds[J]. Science of The Total Environment, 2020,741:140-148.
[32]
Speckbacher V, Zeilinger S, Zimmermann S, et al. Monitoring the volatile language of fungi using gas chromatography-ion mobility spectrometry[J]. Analytical and Bioanalytical Chemistry, 2021, 413(11):3055-3067.
[33]
Larsen T O, Frisvad J C. Comparison of different methods for collection of volatile chemical markers from fungi[J]. Journal of Microbiology Method, 1995,24(2):135-144.
[34]
Stover R H, Freiberg S R. Effect of carbon dioxide on multiplication of fusarium in soil[J]. Nature, 1958,181(4611):788-789.
[35]
Calvo A M, Wilson R A, Bok J W, et al. Relationship between secondary metabolism and fungal development[J]. Microbiology and Molecular Biology Reviews, 2002,66(3):447-459.
[36]
Polizzi V, Adams A, De Saeger S, et al. Influence of various growth parameters on fungal growth and volatile metabolite production by indoor molds[J]. Science of The Total Environment, 2012,414:277-286.
[37]
Pawliszyn J. Handbook of solid phase microextraction[M]. Canada:Chemical Industry Press, 2012:42-50.
[38]
Martos P A, Saraullo A, Pawliszyn J. Estimation of air/coating distribution coefficients for solid phase microextraction using retention indexes from linear temperature-programmed capillary gas chromatography. Application to the sampling and analysis of total petroleum hydrocarbons in air[J]. Analytical Chemistry, 1997,69(3):402-408.
[39]
Schleibinger H, Laussmann D, Brattig C, et al. Emission patterns and emission rates of MVOC and the possibility for predicting hidden mold damage?[J]. Indoor Air, 2005,15:98-104.