Research progress on the mechanisms of iron-assisted anaerobic ammonia oxidation process
WANG Qian1, HU Jia-yuan1, LI Tian-hao1, WANG Cai-xia1, CHEN Jie1, SHEN Yao-liang1,2, LIU Wen-ru1,2
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215000, China; 2. National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215000, China
Abstract：How to promote the proliferation and metabolism of anammox bacteria, improve the nitrogen removal efficiency of anammox process, and ensure the long-term stable operation of the process are the hotspots of anammox research. Iron-assisted anammox is the most widely studied measure with high economical and practical potential. This paper briefly describes the iron-assisted anammox technology and its nitrogen removal performance. The mechanisms of iron-assisted anammox process in promoting anammox cells growth and enhancing systems nitrogen removal performance are summarized from the aspects of optimizing the habitats of anammox bacteria, stimulating the secretion of extracellular polymer, accelerating the accumulation of denitrification functional bacteria, inducing the evolution of cell microstructure, regulating the expression of key enzymes and functional genes, promoting signal molecular synthesis, and enhancing abiotic nitrogen removal. Finally, the iron-assisted anammox technology is summarized and prospected, and the concept of waste iron scrap-anammox process is proposed.
王倩, 胡嘉源, 李天皓, 王彩霞, 陈洁, 沈耀良, 刘文如. 铁强化厌氧氨氧化脱氮机理研究进展[J]. 中国环境科学, 2022, 42(11): 5153-5162.
WANG Qian, HU Jia-yuan, LI Tian-hao, WANG Cai-xia, CHEN Jie, SHEN Yao-liang, LIU Wen-ru. Research progress on the mechanisms of iron-assisted anaerobic ammonia oxidation process. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5153-5162.
Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[2]
Oshiki M, Satoh H, Okabe S. Ecology and physiology of anaerobic ammonium oxidizing bacteria[J]. Environmental Microbiology, 2016,18(9):2784-2796.
[3]
Feng Y, Zhao Y P, Guo Y Z, et al. Microbial transcript and metabolome analysis uncover discrepant metabolic pathways in autotrophic and mixotrophic anammox consortia[J]. Water Research, 2018,128:402-411.
[4]
Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences-An application survey[J]. Water Research, 2014,55:292-303.
[5]
Cao Y S, Hong K B, van Loosdrecht M, et al. Mainstream partial nitritation and anammox in a 200,000m(3)/day activated sludge process in Singapore:Scale-down by using laboratory fed-batch reactor[J]. Water Science and Technology, 2016,74(1):48-56.
[6]
付昆明,付巢,李慧,等.主流厌氧氨氧化工艺的运行优化及其微生物的群落变迁[J]. 环境科学, 2018,39(12):5596-5604. Fu K M, Fu C, Li H, et al. Optimization of the mainstream anaerobic ammonia oxidation process and its changes of the microbial community[J]. Environmental Science, 2018,39(12):5596-5604.
[7]
Liu S T, Yang F L, Meng F A, et al. Enhanced anammox consortium activity for nitrogen removal:Impacts of static magnetic field[J]. Journal of Biotechnology, 2008,138(3/4):96-102.
[8]
Qiao S, Yin X, Zhou J T, et al. Inhibition and recovery of continuous electric field application on the activity of anammox biomass[J]. Biodegradation, 2014,25(4):505-513.
[9]
Cheng B N, Bao J G, Du J K, et al. Application of electric fields to mitigate inhibition on anammox consortia under long-term tetracycline stress[J]. Bioresource Technology, 2021,341:125730.
[10]
Wang T, Zhang D D, Sun Y T, et al. Using low frequency and intensity ultrasound to enhance start-up and operation performance of anammox process inoculated with the conventional sludge[J]. Ultrasonics Sonochemistry, 2018,42:283-292.
[11]
Yuan L Z, Wang T, Xing F H, et al. Enhancement of anammox performances in an ABR at normal temperature by the low-intensity ultrasonic irradiation[J]. Ultrasonics Sonochemistry, 2021,73:105468.
[12]
Ferousi C, Lindhoud S, Baymann F, et al. Iron assimilation and utilization in anaerobic ammonium oxidizing bacteria[J]. Current Opinion in Chemical Biology, 2017,37:129-136.
[13]
Mak C Y, Lin J G, Chen W H, et al. The short-and long-term inhibitory effects of Fe(II) on anaerobic ammonium oxidizing (anammox) process[J]. Water Science and Technology, 2019,79(10):1860-1867.
[14]
Maalcke W J, Reimann J, de Vries S, et al. Characterization of anammox hydrazine dehydrogenase, a key N-2-producing enzyme in the global nitrogen cycle[J]. Journal of Biological Chemistry, 2016,291(33):17077-17092.
[15]
Kartal B, Keltjens J T. Anammox biochemistry:a tale of heme c proteins[J]. Trends in Biochemical Sciences, 2016,41(12):998-1011.
[16]
董子阳,胡宝兰,韩佳慧.厌氧氨氧化细菌Candidatus Kuenenia stuttgartiensis铁的吸收利用研究进展[J]. 微生物学通报, 2021, 48(5):1780-1787. Dong Z Y, Hu B L, Han J H, et al. Research progress in the uptake and utilization of iron by the anaerobic ammonium-oxidizing bacterium Candidatus Kuenenia stuttgartiensis[J]. Microbiology China, 2021, 48(5):1780-1787.
[17]
Peng M W, Qi J, Yan P, et al. Insight into the structure and metabolic function of iron-rich nanoparticles in anammox bacteria[J]. Science of the Total Environment, 2022,806:150879.
[18]
Qiao S, Bi Z, Zhou J T, et al. Long term effects of divalent ferrous ion on the activity of anammox biomass[J]. Bioresource Technology, 2013,142:490-497.
[19]
Bi Z, Zhang W J, Song G, et al. Iron-dependent nitrate reduction by anammox consortia in continuous-flow reactors:A novel prospective scheme for autotrophic nitrogen removal[J]. Science of the Total Environment, 2019,692:582-588.
[20]
Sindhu L, Niu K, Liu X, et al. Effect of Fe2+ addition on anammox consortia, nitrogen removal performance and functional genes analysis during start-up of anammox process[J]. Journal of Water Process Engineering, 2021,43:102251.
[21]
Huang X L, Gao D W, Peng S, et al. Effects of ferrous and manganese ions on anammox process in sequencing batch biofilm reactors[J]. Journal of Environmental Sciences, 2014,26(5):1034-1039.
[22]
Yan Z C, Shen L, Jiao L Z, et al. Effect of Fe (II) on nitrogen removal of anammox under organic matter inhibition[J]. Journal of Water Process Engineering, 2022,46:102632.
[23]
Tang S M, Xu Z H, Liu Y L, et al. Performance, kinetics characteristics and enhancement mechanisms in anammox process under Fe(II) enhanced conditions[J]. Biodegradation, 2020,31:221-234.
[24]
Mishra P, Burman I, Sinha A. Performance enhancement and optimization of the anammox process with the addition of iron[J]. Environmental Technology, 2021,42(26):4158-4169.
[25]
Wang X, Shu D T, Yue H. Taxonomical and functional microbial community dynamics in an Anammox-ASBR system under different Fe (III) supplementation[J]. Applied Microbiology and Biotechnology, 2016,100(23):10147-10163.
[26]
马娇,曾天续,宋珺,等.纳米单质铁对厌氧氨氧化脱氮性能的影响[J]. 中国环境科学, 2022:42(6):2619-2627. Ma J, Zeng T X, Song J, et al. Effect of nanoscale zero-valent iron on the denitrification performance of anaerobic ammonia oxidation[J]. China Environmental Science, 2022:42(6):2619-2627.
[27]
Ren L F, Ni S Q, Liu C, et al. Effect of zero-valent iron on the start-up performance of anaerobic ammonium oxidation (anammox) process[J]. Environmental Science and Pollution Research, 2015, 22(4):2925-2934.
[28]
Zhang X J, Wei D H, Zhang H, et al. Comprehensive analysis of the impacts of iron-based nanoparticles and ions on anammox process[J]. Biochemical Engineering Journal, 2022,180:108371.
[29]
Liu T, Tian R Q, Li Q, et al. Strengthened attachment of anammox bacteria on iron-based modified carrier and its effects on anammox performance in integrated floating-film activated sludge (IFFAS) process[J]. Science of The Total Environment, 2021,787:147679.
[30]
Wang H, Fan Y F, Zhou M D, et al. Function of Fe(III)-minerals in the enhancement of anammox performance exploiting integrated network and metagenomics analyses[J]. Water Research, 2022,210:117998.
[31]
Viet T N, Behera S K, Ji W K, et al. Effects of oxidation reduction potential and organic compounds on anammox reaction in batch cultures[J]. Environmental Engineering Research, 2008,13(4):359-360.
[32]
Suneethi S, Joseph K. Batch culture enrichment of anammox populations from anaerobic and aerobic seed cultures[J]. Bioresource Technology, 2011,102(2):585-591.
[33]
Daverey A, Chen Y C, Sung S W, et al. Effect of zinc on anammox activity and performance of simultaneous partial nitrification, anammox and denitrification (SNAD) process[J]. Bioresource Technology, 2014,165:105-110.
[34]
Guo B B, Chen Y H, Lv L, et al. Transformation of the zero valent iron dosage effect on anammox after long-term culture:From inhibition to promotion[J]. Process Biochemistry, 2019,78:132-139.
[35]
Gao F, Zhang H M, Yang F L, et al. The effects of zero-valent iron (ZVI) and ferroferric oxide (Fe3O4) on anammox activity and granulation in anaerobic continuously stirred tank reactors (CSTR)[J]. Process Biochemistry, 2014,49(11):1970-1978.
[36]
Tomaszewski M, Cema G, Ziembinska-Buczynska A. Influence of temperature and pH on the anammox process:A review and meta-analysis[J]. Chemosphere, 2017,182:203-214.
[37]
Li J, Feng L, Biswal B K, et al. Bioaugmentation of marine anammox bacteria (MAB)-based anaerobic ammonia oxidation by adding Fe(III) in saline wastewater treatment under low temperature[J]. Bioresource Technology, 2020,295:122292.
[38]
Feng L, Li J, Ma H R, et al. Effect of Fe(II) on simultaneous marine anammox and Feammox treating nitrogen-laden saline wastewater under low temperature:Enhanced performance and kinetics[J]. Desalination, 2020,478:114287.
[39]
Bi Z, Huang Y, Zhang W J, et al. Impacts of chosen parameters on fe-dependent nitrate reduction in anammox consortia:performance and bioactivity[J]. Water, 2020,12(5):1379.
[40]
Li Z X, Peng Y Z, Gao H J. Enhanced long-term advanced denitrogenation from nitrate wastewater by anammox consortia:Dissimilatory nitrate reduction to ammonium (DNRA) coupling with anammox in an upflow biofilter reactor equipped with EDTA-2Na/Fe(II) ratio and pH control[J]. Bioresource Technology, 2020,305:123083.
[41]
Oshiki M, Ishii S, Yoshida K, et al. Nitrate-dependent ferrous iron oxidation by anaerobic ammonium oxidation (anammox) bacteria[J]. Applied and Environmental Microbiology, 2013,79(13):4087-4093.
[42]
Yan Y, Wang Y Y, Wang W G, et al. Comparison of short-term dosing ferrous ion and nanoscale zero-valent iron for rapid recovery of anammox activity from dissolved oxygen inhibition[J]. Water Research, 2019,153:284-294.
[43]
Zhang D J, Ren L L, Yao Z B, et al. Removal of nitrogen oxide based on anammox through Fe(II)EDTA absorption[J]. Energy & Fuels, 2017,31(7):7247-7255.
[44]
李亚峰,马晨曦,张驰.UASBB厌氧氨氧化反应器处理污泥脱水液的影响因素研究[J]. 环境科学, 2014,35(8):3044-3051. Li Y F, Ma C X, Zhang C, et al. Influencing factors of sludge liquor treatment in UASBB[J]. Environmental Science, 2014,35(8):3044-3051.
[45]
吕娇,楼菊青,徐帆.反硝化型甲烷厌氧氧化(DAMO)系统pH值耦合模型研究[J]. 中国环境科学, 2022,42(2):612-619. Lv J, Lou J Q, Xu F, et al. pH coupling model of denitrifying anaerobic methane oxidation (DAMO) system[J]. China Environmental Science, 2022,42(2):612-619.
[46]
于德爽,李伟刚,李津.ASBR反应器厌氧氨氧化脱氮Ⅰ:工艺特性与控制策略[J]. 中国环境科学, 2013,33(12):2176-2183. Yu D S, Li W G, Li J, et al. Nitrogen removal in the Anammox sequencing batch reactor I:Performance and control strategies[J]. China Environmental Science, 2013,33(12):2176-2183.
[47]
Gonzalez-Brambila M, Monroy O, Lopez-Isunza F. Experimental and theoretical study of membrane-aerated biofilm reactor behavior under different modes of oxygen supply for the treatment of synthetic wastewater[J]. Chemical Engineering Science, 2006,61(16):5268-5281.
[48]
Zhang Q, Zhang X, Bai Y H, et al. Exogenous extracellular polymeric substances as protective agents for the preservation of anammox granules[J]. Science of the Total Environment, 2020,747:141464.
[49]
Miao L, Zhang Q, Wang S Y, et al. Characterization of EPS compositions and microbial community in an Anammox SBBR system treating landfill leachate[J]. Bioresource Technology, 2018,249:108-116.
[50]
Yang H, Deng L W, Xiao Y Q, et al. Construction of autotrophic nitrogen removal system based on zero-valent iron (ZVI):performance and mechanism[J]. Water Science and Technology, 2020,82(12):2990-3002.
[51]
Wang Z B, Liu X L, Ni S Q, et al. Nano zero-valent iron improves anammox activity by promoting the activity of quorum sensing system[J]. Water Research, 2021,202:117491.
[52]
王海月,彭玲,毛念佳,等.三价铁对有机物存在下厌氧氨氧化脱氮的影响[J]. 中国环境科学, 2021,41(4):1672-1680. Wang H Y, Peng L, Mao N J, et al. Effect of Fe3+ on nitrogen removal of anammox in the presence of organic matter[J]. China Environmental Science, 2021,41(4):1672-1680.
[53]
Zhang S Q, Zhang L Q, Yao H N, et al. Responses of anammox process to elevated Fe(III) stress:Reactor performance, microbial community and functional genes[J]. Journal of Hazardous Materials, 2021,414:125051.
[54]
Lawson C E, Wu S, Bhattacharjee A S, et al. Metabolic network analysis reveals microbial community interactions in anammox granules[J]. Nature Communications, 2017,8:15416.
[55]
Zhao Y P, Liu S F, Jiang B, et al. Genome-centered metagenomics analysis reveals the symbiotic organisms possessing ability to cross-feed with anammox bacteria in anammox consortia[J]. Environmental Science & Technology, 2018,52(19):11285-11296.
[56]
Zhu T T, Lai W X, Zhang Y B, et al. Feammox process driven anaerobic ammonium removal of wastewater treatment under supplementing Fe(III) compounds[J]. Science of the Total Environment, 2022,804:149965.
[57]
Yang Y F, Jin Z, Quan X, et al. Transformation of nitrogen and iron species during nitrogen removal from wastewater via feammox by adding ferrihydrite[J]. ACS Sustainable Chemistry & Engineering, 2018,6(11):14394-14402.
[58]
张蕾,郑平,胡安辉.铁离子对厌氧氨氧化反应器性能的影响[J]. 环境科学学报, 2009,29(8):1629-1634. Zhang L, Zheng P, Hu A H, et al. Effect of ferrous ion on the performance of an anammox reactor[J]. Acta Scientiae Circumstaniae, 2009,29(8):1629-1634.
[59]
Harrison P M. Iron storage in bacteria[J]. Nature, 1979,279:15-16.
[60]
van Niftrik L, Geerts W, van Donselaar E G, et al. Combined structural and chemical analysis of the anammoxosome:A membrane-bounded intracytoplasmic compartment in anammox bacteria[J]. Journal of Structural Biology, 2008,161(3):401-410.
[61]
Giessen T W. Encapsulins:microbial nanocompartments with applications in biomedicine, nanobiotechnology and materials science[J]. Current Opinion in Chemical Biology, 2016,34:1-10.
[62]
Martin W. Evolutionary origins of metabolic compartmentalization in eukaryotes[J]. Philosophical Transactions of the Royal Society B-Biological Sciences, 2010,365(1541):847-855.
[63]
Shu D T, He Y L, Yue H, et al. Effects of Fe(II) on microbial communities, nitrogen transformation pathways and iron cycling in the anammox process:Kinetics, quantitative molecular mechanism and metagenomic analysis[J]. RSC Advances, 2016,6(72):68005-68016.
[64]
Chen Y, Jia F X, Liu Y J, et al. The effects of Fe(III) and Fe(II) on anammox process and the Fe-N metabolism[J]. Chemosphere, 2021,285:131322.
[65]
Wang H Y, Peng L, Mao N J, et al. Effects of Fe3+ on microbial communities shifts, functional genes expression and nitrogen transformation during the start-up of Anammox process[J]. Bioresource Technology, 2021,320:124326.
[66]
Kartal B, de Almeida N M, Maalcke W J, et al. How to make a living from anaerobic ammonium oxidation[J]. FEMS Microbiology Reviews, 2013,37(3):428-461.
[67]
Kartal B, Maalcke W J, de Almeida N M, et al. Molecular mechanism of anaerobic ammonium oxidation[J]. Nature, 2011,479:127-159.
[68]
Han L C, Yang L, Wang H B, et al. Sustaining reactivity of Fe-0for nitrate reduction via electron transfer between dissolved Fe2+ and surface iron oxides[J]. Journal of Hazardous Materials, 2016,308:208-215.
[69]
张文静,黄勇,毕贞,等.ANAMMOX菌铁自养反硝化工艺的稳定性[J]. 环境科学, 2019,40(7):3201-3207. Zhang W J, Huang Y, Bi Z, et al. Stability of ZVI-dependent autotrophic denitrification by anammox bacteria[J]. Environmental Science, 2019,40(7):3201-3207.
[70]
Li X, Yuan Y, Huang Y. Enhancing the nitrogen removal efficiency of a new autotrophic biological nitrogen-removal process based on the iron cycle:Feasibility, progress, and existing problems[J]. Journal of Cleaner Production, 2021,317:128499.
[71]
Shaw D R, Ali M, Katuri K P, et al. Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria[J]. Nature Communications, 2020,11(1):2058.
[72]
Maddela N R, Sheng B B, Yuan S S, et al. Roles of quorum sensing in biological wastewater treatment:A critical review[J]. Chemosphere, 2019,221:616-629.
[73]
Lorite G S, de Souza A A, Neubauer D, et al. On the role of extracellular polymeric substances during early stages of Xylella fastidiosa biofilm formation[J]. Colloids and Surfaces B-Biointerfaces, 2013,102:519-525.
[74]
Voberkova S, Hermanova S, Hrubanova K, et al. Biofilm formation and extracellular polymeric substances (EPS) production by Bacillus subtilis depending on nutritional conditions in the presence of polyester film[J]. Folia Microbiologica, 2016,61(2):91-100.
[75]
Zhao R, Zhang H M, Zhang F, et al. Fast start-up anammox process using Acyl-homoserine lactones (AHLs) containing supernatant[J]. Journal of Environmental Sciences, 2018,65:127-132.
[76]
Wu D L, Shao B B, Fu M Y, et al. Denitrification of nitrite by ferrous hydroxy complex:Effects on nitrous oxide and ammonium formation[J]. Chemical Engineering Journal, 2015,279:149-155.
[77]
Kartal B, Kuypers M, Lavik G, et al. Anammox bacteria disguised as denitrifiers:nitrate reduction to dinitrogen gas via nitrite and ammonium[J]. Environmental Microbiology, 2007,9(3):635-642.
[78]
Duan H R, Ye L, Erler D, et al. Quantifying nitrous oxide production pathways in wastewater treatment systems using isotope technology-A critical review[J]. Water Research, 2017,122:96-113.
[79]
Zhou B R, Chen G J, Dong C F, et al. The short-term and long-term effects of Fe(II) on the performance of anammox granules[J]. Water Environment Research, 2021,93(9):1651-1659.
[80]
李天皓,徐云翔,郭之晗,等.低温下废铁屑对厌氧氨氧化系统的影响[J]. 中国环境科学, 2022,42(4):1688-1695. Li T H, Xu Y X, Guo Z H, et al. Effect of iron scraps on anammox systems at low temperature[J]. China Environmental Science, 2022,42(4):1688-1695.
