Abstract：The preservation of anammox microorganisms is essential since it affects the subsequent reactor performance in nitrogen removal. The characters of anammox biofilm and anammox granular sludge at low temperature preservation were examined in this study. Results showed that after 64days preservation at 4℃, the specific anammox activities were found to be (351.4±14.5) mgN/(gVSS×d) for anammox biofilm and (32.3±2.7) mgN/(gVSS×d) for anammox granular sludge, which were 62.1% and 6.0% of those before preservation. Their EPS content decreased to (18.4±0.3) and (13.3±1.5) mg/gVSS, respectively. Moreover, the abundance of functional anammox genus, Candidatus Kuenenia, decreased from 16.3% to 6.1% in anammox biofilm and decreased from 16.8% to 1.6% in anammox granular sludge. Their anammox gene abundance also decreased to (1.48±0.29)×108and (5.05±1.53)×107gene copies/gVSS, respectively. During the reactivation of anammox process by using those two preserved anammox microorganisms, the time used to reach the NRR of 0.54kgN/m3was lowered by 15days for anammox biofilm compared to that of anammox granular sludge showing the anammox biofilm was a better preservation form for anammox microorganisms than anammox granular sludge.
彭正华, 杨本芹, 杨志宏, 詹友生, 潘学军. 不同形态厌氧氨氧化污泥低温保存的性能差异研究[J]. 中国环境科学, 2023, 43(2): 658-666.
PENG Zheng-hua, YANG Ben-qin, YANG Zhi-hong, ZHAN You-sheng, PAN Xue-jun. Characterization of anammox biofilm and anammox granular sludge at low temperature preservation. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 658-666.
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