长期复合重金属污染对土壤解磷微生物的影响

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摘要为进一步了解长期重金属污染压力下解磷微生物群落分布及潜在功能的变化,采用宏基因组学技术系统分析了北京某金矿周边土壤中含phoD基因或pqqE基因的解磷微生物对不同程度的复合重金属污染的响应.结果表明:重金属对解磷微生物确有影响,随重金属浓度增加,解磷微生物相对丰度逐渐降低,酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)是重金属复合污染土壤中的解磷微生物的优势菌门.重金属是与解磷微生物群落最显著相关的因素,重金属As、Pb、Zn对解磷微生物影响较大,Nitrospirae、Acidobacteria、Gemmatimonadete、Proteobacteria更易受到重金属的影响发生群落结构改变,其中Nitrospirae对Cr、Cu、Pb、Zn、As、Cd多种重金属表现出敏感性.有效磷浓度与解有机磷微生物多样性呈显著正相关关系(P<0.05),碱性磷酸酶活性与解无机磷微生物多样性显著负相关,而土壤有机质、总氮、pH值对重金属污染土壤中解磷微生物多样性无显著影响.研究结果为进一步探索长期重金属污染压力下解磷微生物的生态适应机制提供参考.

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	刘荣灿
	刘晓霞
	冯洋
	赵乾程
	胡雪松
	杜闫彬
	于彩虹

关键词 ：解磷微生物, 重金属, phoD, pqqE

Abstract：In order to further understand the changes of distribution and potential functions of phosphorus solubilizing microorganisms under long-term heavy metal pollution pressure, the response of phosphorus solubilizing microorganisms containing phoD gene or pqqE gene in the soil around a gold mine in Beijing to different levels of combined heavy metal pollution was systematically analyzed by using macrogenomics technology. It is showed that phosphorus solubilizing microorganisms was affected by heavy metals. With heavy metal concentration increasing, the relative abundance of phosphorus solubilizing microorganisms gradually decreased. Acidobacter and Actinobacteria were the dominant bacteria of phosphorus solubilizing microorganisms in heavy metal contaminated soil. Heavy metals were the most significantly related factors to the phosphorus solubilizing microbial community. The phosphorus solubilizing microorganisms were greatly affected by heavy metals As, Pb, Zn. Heavy metals were susceptibly influence Nitrospirae, Acidobacteria, Gemmatimonadete, Proteobateria, which changed the community structure. Nitrospirae was sensitive to Cr, Cu, Pb, Zn, As, Cd. The concentration of available phosphorus was significantly positively related to the diversity of phosphorus solubilizing microorganisms (P<0.05), while Alkaline phosphatase activity was significantly negatively correlated with the diversity of phosphorus solubilizing microorganisms. However, soil organic matter, total nitrogen and pH had no significant effect on the diversity of phosphorus solubilizing microorganisms in heavy metal contaminated soil. The results provided a reference for further exploring the ecological adaptation mechanism of phosphorus releasing microorganisms under long-term heavy metal pollution pressure.

Key words： phosphate solubilizing microorganisms heavy metals phoD pqqE

收稿日期: 2022-06-27

PACS:

X53

通讯作者: 于彩虹,教授,caihongyu@cumtb.edu.cn E-mail: caihongyu@cumtb.edu.cn

作者简介: 刘荣灿(1998-),女,山东菏泽人,中国矿业大学(北京)研究生,主要研究方向土壤重金属污染修复.发表论文1篇.

引用本文:

刘荣灿, 刘晓霞, 冯洋, 赵乾程, 胡雪松, 杜闫彬, 于彩虹. 长期复合重金属污染对土壤解磷微生物的影响[J]. 中国环境科学, 2023, 43(2): 915-926. LIU Rong-can, LIU Xiao-xia, FENG Yang, ZHAO Qian-cheng, HU Xue-song, DU Yan-bin, YU Cai-hong. Effects of long-term combined heavy metal pollution on soil phosphate solubilizing microorganisms. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 915-926.

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