Isolation and identification of antimony-oxidizing bacterium Pseudomonas sp. AO-1 and its oxidation properties for Sb(III)
LONG Pei1, DENG Ren-jian1, YANG Yu1, JIN Gui-zhong2, HUANG Zhong-jie1, ZHOU Xin-he3, WANG Xi-feng1, WANG Chuang1
1. School of Civil Engineering, Hunan University of Science and Technology, Xiangtan 411201, China; 2. Xikuangshan Shanxing Antimony Industry Co., Ltd, Loudi 417500, China; 3. Environmental company of China National Administration of Coal Geology, Handan 056001, China
Abstract：An antimony-oxidizing bacterium was screened from Xikuangshan by the resistance screening method and identified by molecular biology techniques. And its oxidation properties for Sb(III) and the characteristics of oxidized secondary minerals were investigated. The results showed that the antimony-oxidizing bacterium belonged to the genus Pseudomonas sp., which was named Pseudomonas sp. AO-1 (AO-1). The main factors affecting the oxidation of Sb(III) by AO-1 were solution pH, dissolved oxygen and iron/manganese oxide (elemental iron, FeCl3 and MnO2). In addition, AO-1 could oxidize Sb(III) under both aerobic and anoxic conditions, and the Michaelis-Menten constant and maximum oxidation rate of aerobic oxidation of Sb(III) were 393.05mmol/L and 0.271mmol/(L·min), respectively, presenting strong oxidizing ability to antimony. The combination of AO-1 and iron/manganese oxides could promote Sb(III) oxidation, and the rate of iron/manganese oxides promoting the oxidation of Sb(III) by AO-1 was in the following order: FeCl3 > MnO2 > elemental iron. Furthermore, the coupled oxidation of Sb(III) by AO-1 and iron/manganese oxides generated secondary minerals containing Sb(V), which accelerated Sb(III) oxidation and affected the migration and transformation of antimony in the environment. AO-1indeed exhibits satisfactory antimony oxidation performance, which is of great significance for the biogeochemical transformation of antimony and the application of soil microbial remediation.
隆佩, 邓仁健, 杨宇, 金贵忠, 黄中杰, 周新河, 王西峰, 王闯. 锑氧化菌Pseudomonas sp. AO-1的分离鉴定及其对Sb(III)的氧化性能[J]. 中国环境科学, 2023, 43(2): 904-914.
LONG Pei, DENG Ren-jian, YANG Yu, JIN Gui-zhong, HUANG Zhong-jie, ZHOU Xin-he, WANG Xi-feng, WANG Chuang. Isolation and identification of antimony-oxidizing bacterium Pseudomonas sp. AO-1 and its oxidation properties for Sb(III). CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 904-914.
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