Effects of compound microbial agents on anaerobic treatment of food waste at low temperature
WANG Yu-chen1,2, SU Cheng-yuan1,2, WANG Qin2, DING Feng-xiu2, LIANG Zhu2, WEN Shi-tong2, CHEN Meng-lin2, HUANG Zhi2
1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541004, China; 2. School of Environment and Resources, Guangxi Normal University, Guilin 541004, China
Abstract：In order to improve the efficiency of the anaerobic reactor in the low temperature environment, different volumes of compound microbial agents (0, 1, 2, 5mL) were added to the upflow anaerobic sludge bed (UASB) reactor, and the removal rates of COD and NH3-N, key enzyme activities, microbial community structure and function during the anaerobic digestion process were analyzed. The results showed that the optimum removal rates of COD (42.47%) and NH3-N (63.93%) were obtained in the UASB reactor with 2mL of compound microbial agents. And the relative activities of acetate kinase (AK) and coenzyme F420 in anaerobic sludge were increased by (120.39±4.14)% and (148.63±4.32)% compared with the control group. In addition, the abundance and diversity of microorganisms reached the highest, and the relative abundance of Proteobacteria (15.30%) and Thermotogae (1.13%) were the highest, which played an important role in organic acid production and maintaining the stability of anaerobic digestion process. Meanwhile, compared with the dominant archaea Methanobacterium (30.51%) and Methanothrix (32.80%) in the control group, the relative abundance of Methanobacterium (18.93%) was decreased by adding 2mL compound microbial agents. The relative abundance of Methanothrix (34.32%) was increased, which was favorable for the acetic acid type methanogenesis process.
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