Achieving advanced nitrogen removal via anammox coupled with endogenous denitrification
LIU Wen-long, WANG Jia-ming, LI Jun
Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
Abstract:To achieve advanced nitrogen removal of anaerobic ammonia oxidation (anammox) process, endogenous denitrification was combined with anammox by adding sodium acetate (100mg COD/L) in the anaerobic stage of sequencing batch reactor. The nitrogen removal performance and the variations of microbial community structure in the reactor were also investigated during operation. Results showed a stable and high nitrogen removal efficiency of anammox process was achieved by coupled with endogenous denitrification. The total inorganic nitrogen (TIN) removal efficiency was increased from 79.07% ±2.63% to 97.00% ±1.35% and the effluent TIN concentration was reduced to (3.30 ±1.49)mg/L. Typical cycle data showed that the internal carbon sources such as poly-β-hydroxyalkanoates synthesized in the anaerobic stage could provide electron donors for subsequent endogenous denitrification, meanwhile the reaction rate of anammox was not affected. The nitrogen in the system, calculated by mass balance analysis, was mainly removed by anammox (PAMX:PEDN=98.27% :1.73%). Further, the sodium acetate addition stimulated the proliferation of denitrifying bacteria (i.e. Thauera) in the system. However, with the abudance increase of Denitratosoma capable of endogenous denitrifying capacity, the abundance of Anammox bacteria (i.e. Candidatus Brocadia) was restored, and therefore the advanced nitrogen removal of anammox system was realized.
刘文龙, 王佳铭, 李军. 耦合内源反硝化实现厌氧氨氧化工艺深度脱氮[J]. 中国环境科学, 2023, 43(4): 1735-1743.
LIU Wen-long, WANG Jia-ming, LI Jun. Achieving advanced nitrogen removal via anammox coupled with endogenous denitrification. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1735-1743.
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