耦合内源反硝化实现厌氧氨氧化工艺深度脱氮

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摘要为实现厌氧氨氧化(Anammox)工艺的深度脱氮,在厌/缺氧运行序批式反应器(SBR)的厌氧段投加乙酸钠(100mg COD/L)实现了内源反硝化与Anammox的协同脱氮,并考察了反应器的脱氮性能和微生物种群结构变化.结果表明,耦合内源反硝化可明显提升Anammox工艺的脱氮性能,系统的总无机氮(TIN)去除率由79.07%±2.63%提高至97.00%±1.35%,出水TIN降低为(3.30±1.49)mg/L.典型周期数据表明厌氧段合成的内碳源如聚羟基脂肪酸酯可为后续内源反硝化作用提供电子供体,但不对Anammox的反应速率产生影响,基于物料平衡分析表明系统的氮素主要通过Anammox作用去除(P_AMX:P_EDN=98.27%:1.73%).乙酸钠的投加刺激了系统中反硝化菌Thauera的大量增殖,但随着具有内源反硝化能力的Denitratisoma丰度上升,促进了Anammox菌Candidatus Brocadia的丰度恢复,实现了Anammox系统的深度脱氮.

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关键词 ：内源反硝化, 厌氧氨氧化, SBR, 深度脱氮, 菌群结构

Abstract：To achieve advanced nitrogen removal of anaerobic ammonia oxidation (anammox) process, endogenous denitrification was combined with anammox by adding sodium acetate (100mg COD/L) in the anaerobic stage of sequencing batch reactor. The nitrogen removal performance and the variations of microbial community structure in the reactor were also investigated during operation. Results showed a stable and high nitrogen removal efficiency of anammox process was achieved by coupled with endogenous denitrification. The total inorganic nitrogen (TIN) removal efficiency was increased from 79.07% ±2.63% to 97.00% ±1.35% and the effluent TIN concentration was reduced to (3.30 ±1.49)mg/L. Typical cycle data showed that the internal carbon sources such as poly-β-hydroxyalkanoates synthesized in the anaerobic stage could provide electron donors for subsequent endogenous denitrification, meanwhile the reaction rate of anammox was not affected. The nitrogen in the system, calculated by mass balance analysis, was mainly removed by anammox (P_AMX:P_EDN=98.27% :1.73%). Further, the sodium acetate addition stimulated the proliferation of denitrifying bacteria (i.e. Thauera) in the system. However, with the abudance increase of Denitratosoma capable of endogenous denitrifying capacity, the abundance of Anammox bacteria (i.e. Candidatus Brocadia) was restored, and therefore the advanced nitrogen removal of anammox system was realized.

Key words： endogenous denitrification Anammox SBR advanced nitrogen removal microbial community structure

收稿日期: 2022-08-27

PACS:

X703.1

基金资助:国家自然科学基金项目(52000157);浙江省基础公益研究计划项目(LQ21E080013)

通讯作者: 李军,教授,tanweilijun@zjut.edu.cn E-mail: tanweilijun@zjut.edu.cn

作者简介: 刘文龙(1990-),男,浙江湖州人,副研究员,博士,主要从事污水处理与资源化研究.发表论文10余篇.lwl2019@zjut.edu.cn.

引用本文:

刘文龙, 王佳铭, 李军. 耦合内源反硝化实现厌氧氨氧化工艺深度脱氮[J]. 中国环境科学, 2023, 43(4): 1735-1743. LIU Wen-long, WANG Jia-ming, LI Jun. Achieving advanced nitrogen removal via anammox coupled with endogenous denitrification. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1735-1743.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I4/1735

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