Abstract：In this research, a strain of bacteria with vanadium reduction ability was isolated from the vanadium contaminated soil, and the influence of different V(V) concentration, inoculum amount and pH value on V(V) reduction was investigated. Meanwhile, the extracellular and intracellular V(V) reduction by strain and the enzyme activity changes involved in V(V) reduction were characterized to reveal the mechanism of V(V) bioreduction. The results showed that strain NC1-2 was identified as a strain of Enterobacter kobei. When initial V(V) concentration was 160mg/L, the reduction rate of V(V) by the strain was 96.29%. Increase bacterial inoculation accelerated V(V) reduction by the strain, and pH 8.0 was the optimal condition. With decreased the cell membrane permeability, the reduction rate of V(V) increased from 71.2% to 75.0%. There were differences in the reduction of V(V) by different subcellular fractions. It was found that the reduction rates of V(V) by extracellular exudates and cytoplasmic fractions were 41.71% and 80.17%, respectively; No V(V) reduction occurred in the cell membrane fraction. The nitrite reductase (NIR) activity and reduced nicotinamide adenine dinucleotide (NADH) content were increased to different degrees during the reduction of V(V) by the strains. Fourier transform infrared spectroscopy (FTIR) analysis showed the hydroxyl, carbonyl and amide groups on the surface of bacteria were involved in biosorption. Scanning electron microscope (SEM) showed the precipitation around the bacterial body with V(V) reduction. Energy dispersive spectroscopy (EDS) indicated the presence of vanadium in the precipitate. X-ray photoelectron spectroscopy (XPS) analysis showed that strain NC1-2 reduced V(V) to V(IV). Transmission electron microscope (TEM) results indicated that vanadium was precipitated within the bacteria. In summary, the results suggested that strain NC1-2 was able to reduce V(V) and form insoluble V(IV) precipitates intracellularly and extracellularly, and NIR and NADH were involved in the intracellular biotransformation. The research revealed the characteristics and intrinsic mechanism of V(V) reduction by Enterobacter kobei NC1-2, and preliminarily analyzed its intracellular electron transfer. The isolated strain has good application prospects in vanadium pollution remediation.
周睿, 周雅琪, 王丽丽, 盛米雪, 司友斌. 一株钒还原菌的分离鉴定及V(V)还原机理研究[J]. 中国环境科学, 2023, 43(6): 2926-2937.
ZHOU Rui, ZHOU Ya-qi, WANG Li-li, SHENG Mi-xue, SI You-bin. Isolation and identification of a vanadium reducing bacterium and the mechanism of V(V) bioreduction. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 2926-2937.
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