Unravelling the influencing mechanism of polystyrene microplastics on the algal-bacterial granular sludge systems
XU Hang, ZHANG Bing, SHI Wen-xin
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
Abstract：The algal-bacterial granular sludge (ABGS) system was established by inoculating the mature aerobic granular sludge (AGS) and targeted algae (Chlorella). The effects of polystyrene microplastics (PS-MPs) on the ABGS system was investigated in this study. Results indicated that low concentrations of PS-MPs (1mg/L) had no significant effect on the biomass content, algal growth, sludge settling performance and pollutant removal efficiency of ABGS. Compared with the control group, the biomass content, algal growth and sludge settling performance of ABGS were inhibited under the pressure of high concentrations of PS-MPs (10mg/L and 100mg/L). To be specific, the ratio of chlorophyll α to MLSS decreased by 0.19mg/g, and the average particle size of sludge decreased by 200μm. Meanwhile, the granular sludge structure gradually became loosely and disintegrated. The removal efficiencies of chemical oxygen demand (COD), nitrogen, phosphorus of ABGS system were significantly decreased by 4.83%, 5.06% and 4.11%, respectively. In addition, PS-MPs inhibited the secretion of extracellular polymer substance (EPS) of ABGS, especially for the tryptophan and aromatic proteins. In terms of the microbial community structure, PS-MPs improved the diversity of bacterial species in the ABGS system, but inhibited the growth of functional bacteria (such as Verrucomicrobiota, Cyanobacteria and Chloroflexi), which were not conducive to maintaining the long-term stability of ABGS system.
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