玛曲高寒退化草地土壤-微生物特征及相互作用

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摘要以甘肃省甘南藏族自治州玛曲县4种不同退化程度的高寒草地为研究对象,测定和分析了土壤理化因子以及土壤微生物群落变化特征.结果表明:土壤含水量、土壤有机碳、全氮、全磷的含量随草地退化加剧显著下降(P<0.05),土壤pH值和土壤电导率的变化趋势则与之相反.通过高通量测序,分析得到在无、轻度、中度、重度退化草地的细菌、真菌OTU数量分别为1927、2011、2040、1798以及1492、1511、1383、1466个;无、轻度、中度、重度退化草地特有的细菌、真菌OTU数量分别为588、513、691、409和116、112、73、79个.土壤微生物群落细菌数量占明显优势,多样性随草地退化整体下降.土壤细菌的优势菌群变形菌门在中度退化草地上的相对丰度达到最大为45%;土壤真菌优势菌群子囊菌门在无退化草地上的相对丰度最大为89%.RDA分析中,土壤电导率、有机碳、含水量、全氮、全磷和pH值是影响土壤细菌的主要因子,土壤全磷、全氮和有机碳是影响土壤真菌的主要因子.

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	王敏
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	肖音迪

关键词 ：玛曲县, 退化草地, 土壤微生物, 高通量测序技术, 冗余分析

Abstract：In this paper, four kinds of alpine grassland with different degradation levels in Maqu county, Gannan Tibetan Autonomous Prefecture, Gansu Province, were used as the research objects, and soil physicochemical factors and soil microbial community characteristics were measured and analyzed. The results showed that the soil water content, soil organic carbon, total nitrogen, and total phosphorus decreased significantly (P<0.05) with increased grassland degradation. In contrast, the trends of soil pH and soil electrical conductivity were opposite. Through high-throughput sequencing, we analyzed and obtained the numbers of bacterial and fungal OTUs in no, light, moderate, and severely degraded grassland were 1927, 2011, 2040, 1798 and 1492, 1511, 1383, 1466; the numbers of bacterial and fungal OTUs specific to no, light, moderate and severely degraded grassland were 588, 513, 691, 409 and 116, 112, 73, 79. The number of soil microbial community bacteria dominated significantly, and the diversity decreased with the degradation of grassland as a whole. The relative abundance of the dominant group of soil bacteria, Proteobacteria, reached a maximum of 45% in the moderately degraded grassland; the relative abundance of the dominant group of soil fungi, Ascomycota, reached a maximum of 89% in the non-degraded grassland. Through RDA analysis, it was obtained that soil electrical conductivity, organic carbon, water content, total nitrogen, total phosphorus and pH were the main influence factors of soil bacteria, and soil total phosphorus, total nitrogen, and organic carbon were the main influence factors of soil fungi.

Key words： Maqu county degraded grassland soil microorganisms high-throughput sequencing technology redundancy analysis

收稿日期: 2023-04-28

PACS:

X53

基金资助:甘肃省高校产业支撑计划项目(2023CYZC-21)

通讯作者: 刘旻霞,教授,xiaminl@163.com E-mail: xiaminl@163.com

作者简介: 王敏(1998-),女,山西朔州人,西北师范大学硕士研究生,研究方向为土壤微生物和恢复生态学.17852325851@139.com.

引用本文:

王敏, 刘旻霞, 王千月, 苗乐乐, 杨春亮, 肖音迪. 玛曲高寒退化草地土壤-微生物特征及相互作用[J]. 中国环境科学, 2023, 43(12): 6482-6489. WANG Min, LIU Min-xia, WANG Qian-yue, MIAO Le-le, YANG Chun-liang, XIAOYin-di. Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(12): 6482-6489.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I12/6482

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