Abstract：In this paper, four kinds of alpine grassland with different degradation levels in Maqu county, Gannan Tibetan Autonomous Prefecture, Gansu Province, were used as the research objects, and soil physicochemical factors and soil microbial community characteristics were measured and analyzed. The results showed that the soil water content, soil organic carbon, total nitrogen, and total phosphorus decreased significantly (P<0.05) with increased grassland degradation. In contrast, the trends of soil pH and soil electrical conductivity were opposite. Through high-throughput sequencing, we analyzed and obtained the numbers of bacterial and fungal OTUs in no, light, moderate, and severely degraded grassland were 1927, 2011, 2040, 1798 and 1492, 1511, 1383, 1466; the numbers of bacterial and fungal OTUs specific to no, light, moderate and severely degraded grassland were 588, 513, 691, 409 and 116, 112, 73, 79. The number of soil microbial community bacteria dominated significantly, and the diversity decreased with the degradation of grassland as a whole. The relative abundance of the dominant group of soil bacteria, Proteobacteria, reached a maximum of 45% in the moderately degraded grassland; the relative abundance of the dominant group of soil fungi, Ascomycota, reached a maximum of 89% in the non-degraded grassland. Through RDA analysis, it was obtained that soil electrical conductivity, organic carbon, water content, total nitrogen, total phosphorus and pH were the main influence factors of soil bacteria, and soil total phosphorus, total nitrogen, and organic carbon were the main influence factors of soil fungi.
王敏, 刘旻霞, 王千月, 苗乐乐, 杨春亮, 肖音迪. 玛曲高寒退化草地土壤-微生物特征及相互作用[J]. 中国环境科学, 2023, 43(12): 6482-6489.
WANG Min, LIU Min-xia, WANG Qian-yue, MIAO Le-le, YANG Chun-liang, XIAOYin-di. Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(12): 6482-6489.
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