环洞庭湖土壤抗生素抗性基因分布和潜在风险

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摘要本研究通过实时荧光定量PCR和基于Monte Carlo模拟的概率健康风险评估模型,分析了环洞庭湖流域52个表层土壤点位中8类典型抗生素抗性基因(ARGs,16种ARGs亚型)的分布特征和潜在健康风险.结果表明,环洞庭湖流域周边表层土壤中ARGs总丰度范围为3.29×10⁷~3.73×10⁹copies/g,远高于土壤ARGs背景值(1.80×10⁵~1.35×10⁷copies/g,平均值为3.03×10⁶copies/g).南洞庭湖流域表层土壤中ARGs丰度高于东、西洞庭湖流域.磺胺类、四环素类和氨基糖苷类ARGs是高丰度的ARGs类型,平均丰度分别为1.51×10⁸、1.50×10⁷和2.64×10⁷copies/g,主要ARGs亚型是sul1和dfrA14,均属于磺胺类ARGs.I类整合子基因(intl1)的平均丰度为6.94×10⁸copies/g,高于总ARGs平均丰度.相关性分析表明intl1丰度与dfrA14、tetR和kan基因丰度呈显著正相关(P<0.05),表明intl1通过水平基因转移促进了研究区域表层土壤中ARGs的传播.Monte Carlo模拟表明,研究区域存在64.92%~98.68%概率的ARGs人体暴露剂量高于风险背景值(成人12.56copies/(g·d),儿童38.11copies/(g·d)),表明研究区域土壤ARGs造成的潜在健康风险不可忽视,皮肤接触是ARGs主要暴露途径,磺胺类ARGs是潜在健康风险的主要来源.

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	武晨
	黄凤莲
	刘新刚
	范青青
	陆海楠
	李启武
	郭倩
	李莹
	彭勇春
	李峰

关键词 ：环洞庭湖, 土壤, 抗生素抗性基因, 分布特征, 潜在风险

Abstract：52 surface soil samples were collected around Dongting Lake basin. The distribution characteristics and potential health risks of eight typical antibiotic resistance genes (ARGs, comprising 16subtypes of ARGs) in surface soil were analyzed by real-time fluorescence quantitative PCR (qPCR) and a probability health risk assessment model based on Monte Carlo simulation. The total abundance of ARGs in surface soil of the Dongting Lake Basin ranged from 3.29 × 10⁷ to 3.73 × 10⁹ copies/g, which was significantly higher than the soil background value of ARGs (1.80×10⁵ to 1.35×10⁷ copies/g, with an average of 3.03×10⁶ copies/g). The southern Dongting Lake Basin exhibited higher ARGs abundance in surface soil compared to the eastern and western Dongting Lake Basin. Sulfonamide, tetracycline, and aminoglycoside resistance genes were the predominant ARGs, with average abundances of 1.51 × 10⁸, 1.50 × 10⁷, and 2.64 × 10⁷ copies/g, respectively. The main subtypes of ARGs were sul1and dfrA14, both belonging to sulfonamide resistance genes. The average abundance of class I integron gene (intl1) was 6.94 × 10⁸copies/g, higher than the average abundance of total ARGs in the study area. Correlation analysis showed a significant positive correlation (P < 0.05) between intl1 abundance and the abundances of dfrA14, tetR, and kan genes, indicating that intl1promotes the spread of ARGs in surface soil via horizontal gene transfer. Monte Carlo simulation revealed that there was a probability of 64.92% to 98.68% for human exposure to ARGs exceeding risk background values (adults and children were 12.56 and 38.11copies/(g·d), respectively), indicating that the potential health risks associated with soil-borne ARGs in the study area. Skin contact was identified as the main exposure pathway for ARGs, and sulfonamide resistance genes were identified as the primary contributors to potential health risks of ARGs.

Key words： Dongting Lake Basin soil antibiotic resistance genes distribution characteristics potential risks

收稿日期: 2023-07-29

PACS:

X53

基金资助:国家环境保护城市土壤污染控制与修复工程技术中心开放基金资助项目(USCR-202208);国家环境保护重金属污染监测重点实验室开放基金资助项目(SKLMHM202303);“新污染物的环境健康效应与协同治理技术”湖南省普通高等学校科技创新团队支持项目

作者简介: 武晨(1996-),男,陕西西安人,湘潭大学博士研究生,主要从事农田土壤污染修复和风险管控研究.发表论文3篇.wuchen097@163.com.

引用本文:

武晨, 黄凤莲, 刘新刚, 范青青, 陆海楠, 李启武, 郭倩, 李莹, 彭勇春, 李峰. 环洞庭湖土壤抗生素抗性基因分布和潜在风险[J]. 中国环境科学, 2024, 44(3): 1575-1583. WU Chen, HUANG Feng-lian, LIU Xin-gang, FAN Qing-qing, LU Hai-nan, LI Qi-wu, GUO Qian, LI Ying, PENG Yong-chun, LI Feng. Distributions and potential risks of antibiotic resistance genes in soils around Dongting Lake Basin, China. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1575-1583.

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