Effects of artificial turbulence on physiology and biochemistry of Microcystis aeruginosa
LIU Huan, WANG Na, ZHANG Hai-han, LIU Tao, MA Ben, LIU Xiang, PAN Si-xuan
Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：To explore the mechanism underlying the impact of water disturbance on algal growth and physiology, the growth metabolism and photosynthesis of Microcystis aeruginosa cells were examined under controlled artificial turbulence. The results demonstrated that the uptake of nitrogen and phosphorus by Microcystis aeruginosa cells could be enhanced by the moderate disturbance, consequently stimulating algal growth. Conversely, the growth of algae was inhibited by excessive disturbance. Furthermore, an increase in the photosynthetic activity and rate, as well as the production of adenosine triphosphate (ATP) in algal cells, was observed under moderate disturbance. At 14days of the trial, the effective quantum yield (Y(Ⅱ)) of photosystem II (PS II) in the 400r/min disturbance group was 3.77times higher than that before the disturbance. At 500r/min, enhanced antioxidant enzyme activity was exhibited by Microcystis aeruginosa cells to mitigate the oxidative damage caused by the accumulated reactive oxygen species (ROS), thus ensuring the maintenance of normal cellular processes. Three-dimensional fluorescence analysis results indicated that the production of intracellular organic matter (IOM) in algae could be facilitated by moderate disturbance. Consequently, in-situ algae inhibition could be achieved to a certain extent by turbulence disturbance in the water bodies affected by cyanobacterial blooms.
刘欢, 王娜, 张海涵, 刘涛, 马奔, 刘祥, 潘思璇. 人工紊流对铜绿微囊藻生理生化的影响[J]. 中国环境科学, 2024, 44(12): 6865-6873.
LIU Huan, WANG Na, ZHANG Hai-han, LIU Tao, MA Ben, LIU Xiang, PAN Si-xuan. Effects of artificial turbulence on physiology and biochemistry of Microcystis aeruginosa. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(12): 6865-6873.
[1] 李媛,张家卫,魏杰,等.我国蓝藻水华的发生机理、危害及防控利用研究进展[J]. 微生物学杂志, 2015,35(4):93-97. LI Y, Zhang J W, Wei J, et al. Advances in mechanism of the occurrence, hazard, and prevention /control utilization of cyanophytic blooms in China [J]. Journal of Microbiology, 2015,35(4):93-97. [2] Preece E P, Hardy F J, Moore B C, et al. A review of microcystin detections in Estuarine and Marine waters: Environmental implications and human health risk [J]. Harmful Algae, 2017,61:31-45. [3] 余茂蕾,洪国喜,许海,等.湖泊蓝藻水华对连通河道水质的影响[J]. 环境科学, 2019,40(2):603-613. Xu M L, Hong G X, Xu H, et al. Effects of cyanobacterial bloom in eutrophic lakes on water quality of connected rivers [J]. Environmental Science, 2019,40(2):603-613. [4] Laureano-Rosario A E, McFarland M, Bradshaw D J, et al. Dynamics of microcystins and saxitoxin in the Indian River Lagoon, Florida [J]. Harmful Algae, 2021,103:102012. [5] Ralston D K, Keafer B A, Brosnahan M L, et al. Temperature dependence of an estuarine harmful algal bloom: Resolving interannual variability in bloom dynamics using a degree-day approach [J]. Limnology and Oceanography, 2014,59(4):1112-1126. [6] 李衍庆,黄廷林,张海涵,等.水源水库藻类功能群落演替特征及水质评价[J]. 环境科学, 2020,41(5):2158-2165. Li Y Q, Huang T L, Zhang H H, et al. Succession characteristics of algae functional groups and water quality assessment in a drinking water reservoir [J]. Environmental Science, 2020,41(5):2158-2165. [7] Li J B, Ou R, Liao H Y, et al. Natural lighting enhancing the algae proliferation and nitrogen removal in membrane-aerated bacterial- algal biofilm reactor [J]. Science of the Total Environment, 2022,851(Pt 1):158063-158063. [8] 娄孝飞,杜延生,张海平.基于细胞尺度的光照对四尾栅藻生长的影响[J]. 中国环境科学, 2020,40(11):5020-5026. Lou X F, Du Y S, Zhang H P. Effect of light intensity on the growth of Scenedesmus quadricauda at cell scale [J]. China Environmental Science, 2020,40(11):5020-5026. [9] Zhang H H, Yang Y S, Liu X, et al. Novel insights in seasonal dynamics and co-existence patterns of phytoplankton and micro- eukaryotes in drinking water reservoir, Northwest China: DNA data and ecological model [J]. Science of the Total Environment, 2023, 857:159160. [10] Song K Y, Zhu S F, Lu Y, et al. Modelling the thresholds of nitrogen/phosphorus concentration and hydraulic retention time for bloom control in reclaimed water landscape [J]. Frontiers of Environmental Science & Engineering, 2022,16(10):129. [11] 许海,陈丹,陈洁,等.氮磷形态与浓度对铜绿微囊藻和斜生栅藻生长的影响[J]. 中国环境科学, 2019,39(6):2560-2567. Xu H, Chen D, Chen J, et al. Effects of nitrogen and phosphorus forms and concentrations on the growth of Microcystis aeruginosa and Scenedesmus obliquus [J]. China Environmental Science, 2019,39(6): 2560-2567. [12] Yang G J, Zhong C N, Rui Z, et al. Intermittent disturbance combined N and P adding favor colony size and abundance of Microcystis flos-aquae [J]. Annales de Limnologie - International Journal of Limnology, 2021,57:17. [13] Ren L X, Ding K Q, Hu Z X, et al. Processes and mechanisms of phosphorus mobility among sediment, water, and cyanobacteria under hydrodynamic conditions [J]. Environmental Science and Pollution Research, 2022,29(6):9354-9368. [14] Xu S F, Zhang L Y, Lin K Z, et al. Effects of light and water disturbance on the growth of Microcystis aeruginosa and the release of algal toxins [J]. Water Environment Research, 2021,93(12):2958-2970. [15] 高遐,肖艳,石锦安,等.紊流扰动对水华鱼腥藻细胞生理的影响[J]. 水生生物学报, 2017,41(2):486-490. Gao X, Xiao Y, Shi J A, et al. Effect of small-scale turbulence on cell biology of Anabaena flos-aquae [J]. Acta Hydrobiologica Sinica, 2017,41(2):486-490. [16] Ding Y Q, Qin B Q, Xu H, et al. Effects of sediment and turbulence on alkaline phosphatase activity and photosynthetic activity of phytoplankton in the shallow hyper-eutrophic Lake Taihu, China [J]. Environmental Science and Pollution Research, 2016,23(16):16183-16193. [17] Xiao Y, Li Z, Li C, et al. Effect of small-scale turbulence on the physiology and morphology of two bloom-forming cyanobacteria [J]. PloS One, 2016,11(12):e0168925. [18] Spoljar M, Zhang C, Drazina T, et al. Development of submerged macrophyte and epiphyton in a flow-through system: Assessment and modelling predictions in interconnected reservoirs [J]. Ecological Indicators, 2017,75:145-154. [19] Tan Y, Li J, Zhang L L, et al. Mechanism underlying flow velocity and its corresponding influence on the growth of Euglena gracilis, a dominant bloom species in reservoirs [J]. International Journal of Environmental Research and Public Health, 2019,16(23):4641. [20] 张海涵,王娜,宗容容,等.水动力条件对藻类生理生态学影响的研究进展[J]. 环境科学研究, 2022,35(1):181-190. Zhang H H, Wang N, Zong R R, et al. Research Progress on Influence of Hydrodynamic Conditions on Algal Physiology and Ecology [J]. Research of Environmental Sciences, 2022,35(1):181-190. [21] Pápista É, Ács É, Böddi B. Chlorophyll-a determination with ethanol - a critical test [J]. Hydrobiologia, 2002,485(1-3):191-198. [22] 闫苗苗.水源水库藻类种群时空演替的伴生菌群驱动机制研究[D]. 西安:西安建筑科技大学, 2020. Yan M M. Disentangling the drivers of spatiotemporal succession of phytoplankton from drinking water reservoirs: Insights into compositions and co-occurrence of bacterial communities [D]. Xi'an: Xi'an University of Architecture and Technology, 2020. [23] 刘春晓,王平,李海燕,等.DBP对铜绿微囊藻生长和抗氧化酶的影响[J]. 环境科学与技术, 2015,38(2):7-12. Liu C X, Wang P, Li H Y, et al. Effects of Dibutyl Phthalate on Growth and Enzymatic Antioxidants of Microcytis aeruginosa [J]. Environmental Science & Technology, 2015,38(2):7-12. [24] 韩丽华,杨桂军,刘玉,等.扰动强度对太湖水华微囊藻群体生长和叶绿素荧光的影响[J]. 环境科学研究, 2018,31(2):265-272. Han L H, Yang G J, Liu Y, et al. Effect of disturbance intensity on the growth and chlorophyll fluorescence of Microcystis flos-aquae colony in Lake Taihu [J]. Research of Environmental Sciences, 2018,31(2):265-272. [25] 颜润润,逄勇,赵伟,等.环流型水域水动力对藻类生长的影响[J]. 中国环境科学, 2008,28(9):813-817. Yan R R, Pang Y, Zhao W, et al. Influence of circumfluent type waters hydrodynamic on growth of algae [J]. China Environmental Science, 2008,28(9):813-817. [26] Michels M H A, van der Goot A J, Vermue M H, et al. Cultivation of shear stress sensitive and tolerant microalgal species in a tubular photobioreactor equipped with a centrifugal pump [J]. Journal of Applied Phycology, 2016,28(1):53-62. [27] Song Y, Zhang L L, Li J, et al. Mechanism of the influence of hydrodynamics on Microcystis aeruginosa, a dominant bloom species in reservoirs [J]. Science of the Total Environment, 2018,636:230-239. [28] 江成.水体扰动对藻生长机制与QCS水库富营养化控制的影响研究[D]. 上海:上海交通大学, 2014. Jiang C. Effect of water turbulence on the algal growth mechanism and eutrophication control in QCS reservoir [D]. Shanghai: Shanghai Jiao Tong University, 2014. [29] 江林燕,江成,周伟,等.水体扰动对铜绿微囊藻生长影响的规律及原因[J]. 环境化学, 2012,31(2):216-220. Jiang L Y, Jiang C, Zhou W, et al. Growth of Microcystis aeruginosa under different disturbance [J]. Environmental Chemistry, 2012,31(2): 216-220. [30] Belohlav V, Zakova T, Jirout T, et al. Effect of hydrodynamics on the formation and removal of microalgal biofilm in photobioreactors [J]. Biosystems Engineering, 2020,200:315-327. [31] Zhang H H, Zong R R, He H Y, et al. Effects of hydrogen peroxide on Scenedesmus obliquus: Cell growth, antioxidant enzyme activity and intracellular protein fingerprinting [J]. Chemosphere, 2022,287:132185. [32] 刘国锋,韩士群,刘学芝,等.藻华聚集的环境效应:对漂浮植物水葫芦(Eichharnia crassipes)抗氧化酶活性的影响[J]. 湖泊科学, 2016, 28(1):31-39. Liu G F, Han S Q, Liu X Z, et al. The environmental effects of algae bloom cluster: Impact on the antioxidant enzyme activities of water hyacinth (Eichharnia crassipes) [J]. Journal of Lake Sciences, 2016,28(1):31-39. [33] Song Y, Zhang L L, Li J, et al. Mechanism of the influence of hydrodynamics on Microcystis aeruginosa, a dominant bloom species in reservoirs [J]. Science of the Total Environment, 2018,636:230-239. [34] Li Z, Xiao Y, Yang J, et al. Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence [J]. Chinese Journal of Oceanology and Limnology, 2017,35(6):1409-1416. [35] Huang J, Xi B D, Xu Q J, et al. Experiment study of the effects of hydrodynamic disturbance on the interaction between the cyanobacterial growth and the nutrients [J]. Journal of Hydrodynamics, 2016,28(3):411-422. [36] Blersch D M, Kangas P C, Mulbry W W. Turbulence and nutrient interactions that control benthic algal production in an engineered cultivation raceway [J]. Algal Research, 2013,2(2):107-112. [37] Zhao G X, Gao X P, Zhang C, et al. The effects of turbulence on phytoplankton and implications for energy transfer with an integrated water quality-ecosystem model in a shallow lake [J]. Journal of Environmental Management, 2020,256:109954. [38] Zhang H H, Zhao K X, Liu X, et al. Bacterial community structure and metabolic activity of drinking water pipelines in buildings: A new perspective on dual effects of hydrodynamic stagnation and algal organic matter invasion [J]. Water Research, 2022,225:119161. [39] Klausmeier C A, Litchman E, Daufresne T, et al. Optimal nitrogen- to-phosphorus stoichiometry of phytoplankton [J]. Nature, 2004,429(6988):171-174. [40] Yamashita Y, Panton A, Mahaffey C, et al. Assessing the spatial and temporal variability of dissolved organic matter in Liverpool Bay using excitation-emission matrix fluorescence and parallel factor analysis [J]. Ocean Dynamics, 2011, 61:569-579. [41] Eder A, Weigelhofer G, Pucher M, et al. Pathways and composition of dissolved organic carbon in a small agricultural catchment during base flow conditions [J]. Ecohydrology and Hydrobiology, 2021:1642-3593. [42] Catalán N, Pastor A, Borrego C M, et al. The relevance of environment vs. composition on dissolved organic matter degradation in freshwaters [J]. Limnol Oceanogr, 2021,66:306-320. [43] Chen M, Kim J H, Nam S I, et al. Production of fluorescent dissolved organic matter in Arctic Ocean sediments [J]. Scientific Reports, 2016,16(6):39213.
