Nutrient removal effect of plant+microbial combined ecological ditch under salinity stress
YANG Wen-huan1,2, PENG Yao-hua1,2, XU Yan1,2, YAO Zhi1,2, WANG Le-le3, LI Wei-ping1,2
1. School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, Inner Mongolia, China; 2. Collaborative Innovation Center of Inner Mongolia Autonomous Region for Ecological Protection and Comprehensive Utilization of the Yellow River Basin, Baotou 014010, Inner Mongolia, China; 3. Service Center of Daihai Nature Reserve, Liangcheng County, Ulanqab 013750, Inner Mongolia, China
Abstract：To efficiently remove nutrient salts in high salinity lakes, this study implemented a plant+microbial ditch at Daihai Lake. The nutrient removal efficiency, plant growth, and microbial community dynamics of this ecological ditch was investigated across different salinity gradients. Immobilizing Bacillus amyloliquefaciens on polyurethane sponge within the ditch could significantly promote the growth of plant, enhance the activity of antioxidant stress-related enzymes, and strengthen the ability of plants to absorb and assimilate nutrients. Addiing Bacillus amyloliquefaciens in the ditch could not make it become the absolute dominant genus. but it could improve the microbial community structure, increase the abundance of functional microorganisms involved in nitrogen and phosphorus removal, and enhance the water purification capacity of the ditch. Compared to ecological ditches with plant-only or microorganism-only, the integrated ecological ditch could achieve higher nutrient removal rates across different salinity concentrations. Specifically, over 70% TN, NH4+-N, TP, and CODCr could be removed in the integrated ditch at 15g/L salinity. Compared plant-only ditch, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in plants were increased 33.7%, 43.6%, and 38.7%, respectively, in planted+microbial ditch at 15.0g/L salinity.
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