太湖周边地下水中高硝酸盐负荷促进反硝化功能菌群富集

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摘要反硝化功能菌群在降解硝态氮污染中发挥着重要作用,目前对其内部种间关系与硝酸盐负荷的联系仍了解有限.太湖周边地区地下水资源是重要的储备水源,但部分区域的地下水硝酸盐负荷超出了范围.本文以太湖周边地区14个不同硝酸盐浓度的地下水样品作为研究对象,运用微生物测序技术探究了地下水细菌群落多样性并识别出反硝化功能菌群,通过共现网络分析探究了反硝化功能菌群内部种间关系对硝酸盐负荷的响应.结果表明:高硝态氮组与低硝态氮组间细菌群落组成存在显著差异;细菌群落α多样性、β多样性受到硝酸盐负荷的显著影响;高硝态氮组地下水反硝化功能菌群内部种间关系较低硝态氮组更为密切.本研究表明地下水硝酸盐负荷对反硝化功能菌群内部种间关系具有显著影响,为太湖周边地区地下水反硝化功能菌群与硝态氮的互作机制研究提供了新的见解.

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关键词 ：太湖, 地下水, 微生物多样性, 硝态氮, 共现网络

Abstract：The denitrifying functional bacteria community plays a crucial role in the degradation of nitrate pollution. However, the understanding of the relationships between its interspecies links and nitrate load is still limited. Groundwater resources in the vicinity of Lake Taihu serve as vital reserves, yet in some areas, nitrate load in groundwater exceeds health thresholds. This paper focused on 14 groundwater samples with varying nitrate concentrations from the Lake Taihu area. With microbial sequencing techniques, the diversity of bacterial communities in groundwater was explored, and the denitrifying functional bacteria community was identified. The response of interspecies links within the denitrifying functional bacteria community to nitrate load was investigated by co-occurrence network analysis. The results indicate: Significant differences were found in bacterial community composition between high and low nitrate samples; The α-diversity and β-diversity were highly influenced by nitrate load; Interspecies links within the denitrifying functional bacteria community in the high nitrate samples were denser than those in the low nitrate samples. This study demonstrates that nitrate load in groundwater significantly influences the interspecies links within the denitrifying functional bacteria community, providing new insights into the interplay between groundwater denitrifying functional bacteria community and nitrate load in the Lake Taihu area.

Key words： Lake Taihu groundwater microbial diversity nitrate co-occurrence network

收稿日期: 2024-06-14

PACS:	X172
	X523

基金资助:国家自然科学基金资助项目(42177043)

通讯作者: 陈扣平,副教授,kpchen@nju.edu.cn E-mail: kpchen@nju.edu.cn

作者简介: 王卓群(1999-),女,山东淄博人,硕士研究生,主要从事地下水污染研究.Wangzq_99@163.com.

引用本文:

王卓群, 陈扣平, 吴吉春, 王保战, 柳旭. 太湖周边地下水中高硝酸盐负荷促进反硝化功能菌群富集[J]. 中国环境科学, 2025, 45(1): 477-486. WANG Zhuo-qun, CHEN Kou-ping, WU Ji-chun, WANG Bao-zhan, LIU Xu. High nitrate load in lakeside groundwater around Lake Taihu promotes the enrichment of denitrifying functional bacteria community. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(1): 477-486.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I1/477

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