农村污水间歇断流下滴滤池效能维持及微生物胞外应激机制

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摘要间歇断流下微生物的适应恢复能力是滴滤池稳定运行的关键,该过程与微生物以EPS形式储存的活性物质紧密联系.基于此,对比分析滴滤池稳定运行周期内生物膜结构、EPS组分及其官能团的变化特征,探讨了EPS的应激机制同微生物活性维持与恢复的关联.结果表明,间歇断流下滴滤池的COD、NH₄⁺-N去除率分别达95.56%±1.10%、87.06%±2.08%;受EPS影响,生物膜呈松散孔隙/紧密堆积的形貌结构;微生物在断流期转化SB-EPS内吸附的碳源与LB-EPS内储存的多糖为营养物质,并依靠EPS内疏水基团与架桥作用维护稳定结构,形成了EPS适应饥饿交替的应激机制,进而维持了滴滤池的稳定运行.

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	李亚娇
	蒋小雨
	池玉蕾
	张彬鸿
	任武昂
	丁晓倩
	鞠恺
	金鹏康

关键词 ：生物滴滤池, 间歇断流, 生物膜结构, 胞外聚合物, 应激机制

Abstract：The adaptive resilience of microorganisms is crucial for maintaining the stable operation of Biotrickling filters under intermittent flow interruption. This process is intrinsically related to the ability of microorganism to store active substances as Extracellular Polymeric Substances (EPS). To elucidate this mechanism, a comparative analysis was conducted on biofilm structural characteristics, EPS compositional variations, and functional group transformations during an operation cycle of Biotrickling filters. The correlation between EPS-mediated stress response mechanisms and microbial activity maintenance/recovery was investigated. The results revealed that the removal of COD and NH₄⁺-N reached (95.56±1.10)% and (87.06±2.08)% respectively in the biotrickling filter operated under intermittent flow. Under the regulation of EPS, the biofilm showed a loose and porous structure. During flow interruption phases, microorganisms activated starvation adaptation strategies by converting carbon sources adsorbed in SB-EPS and metabolizing polysaccharides stored in SB-EPS. The structure integrity of microorganisms was maintained via synergistic effects of hydrophobic functional groups within EPS and polymer bridging interactions. Accordingly, an EPS-mediated stress adaptation system responsive to starvation-recovery alternations was established, enabling sustainable operation of Biotrickling filters.

Key words： biotrickling filter intermittent flow interruption biofilm structure extracellular polymeric substances stress mechanism

收稿日期: 2024-10-19

PACS:

X703.1

基金资助:国家自然科学基金项目(52370101); 中国联合工程有限公司青年基金项目(QNJJ-PY-2022-27)

作者简介: 李亚娇(1978-),女,辽宁大石桥人,副教授,博士,主要研究方向为水污染控制与防治.65942271@qq.com.

引用本文:

李亚娇, 蒋小雨, 池玉蕾, 张彬鸿, 任武昂, 丁晓倩, 鞠恺, 金鹏康. 农村污水间歇断流下滴滤池效能维持及微生物胞外应激机制[J]. 中国环境科学, 2025, 45(5): 2434-2442. LI Ya-jiao, JIANG Xiao-yu, CHI Yu-lei, ZHANG Bin-hong, REN Wu-ang, DING Xiao-qian, JU Kai, JIN Peng-kang. Sustaining bioreactor efficacy in rural wastewater treatment under intermittent flow interruption: Elucidating microbial EPS-mediated stress adaptation mechanisms. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2434-2442.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I5/2434

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