A “turn-on” fluorescent probe for Al3+ and its biological detection in vivo
YU Yan-chao, FENG Jun-kai, LIU Bo, YOU Jun, WU Wen-ju, JING Jun-kai
Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemicaland Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China
Abstract：A novel bisalicylaldehyde schiff base was designed and synthesized, and its structure was confirmed by NMR and MS. It was found to be highly selective for Al3+ in methanol as a "turn-on" fluorescent probe in fluorescence spectroscopy, and fluorescence intensity enhanced 110-fold after the probe recognized Al3+. Fluorescence titration experiments showed that the probe have an excellent linear relationship with Al3+ concentration range 25~55μmol/L, and the detection limit was calculated to be as low as 5.4×10-9mol/L, which was far below the drinking water restriction of Al3+ by WHO (7.4×10-6 mol/L). HRMS analysis showed the binding stoichiometry between probe and Al3+ was to be 2:1. Moreover, the probe was applied for biological detection in vivo of Al3+ ions, which presented excellent biocompatibility, and could be applied to the detection of Al3+ in water body and organisms.
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