一种turn-on型Al3+荧光探针及其活体生物检测

喻艳超; 冯俊凯; 刘波; 由君; 武文菊; 荆军凯

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中国环境科学 ›› 2020, Vol. 40 ›› Issue (12) : 5422-5427.

环境生态

一种turn-on型Al³⁺荧光探针及其活体生物检测

喻艳超, 冯俊凯, 刘波, 由君, 武文菊, 荆军凯

作者信息 +

A “turn-on” fluorescent probe for Al³⁺ and its biological detection in vivo

YU Yan-chao, FENG Jun-kai, LIU Bo, YOU Jun, WU Wen-ju, JING Jun-kai

Author information +

文章历史 +

摘要

设计、合成一种联水杨醛席夫碱荧光探针，并对其结构表征.光谱实验表明，在甲醇溶液中，该探针可实现对Al³⁺的turn-on检测，在识别Al³⁺后，荧光强度增强约110倍，并具有良好的离子选择性.荧光滴定实验中，Al³⁺浓度在25~55μmol/L范围内，荧光强度与浓度呈良好线性相关，探针对Al³⁺检出限为5.4×10^-9mol/L，低于世界卫生组织对饮用水中Al³⁺含量最低标准（7.4×10^-6mol/L）.高分辨质谱数据表明，探针分子与Al³⁺的络合比例为2：1.在活体生物Al³⁺检测中，探针具有良好的生物相容性，表明其在水体及生物体Al³⁺检测中具有一定的应用前景.

Abstract

A novel bisalicylaldehyde schiff base was designed and synthesized, and its structure was confirmed by NMR and MS. It was found to be highly selective for Al³⁺ in methanol as a "turn-on" fluorescent probe in fluorescence spectroscopy, and fluorescence intensity enhanced 110-fold after the probe recognized Al³⁺. Fluorescence titration experiments showed that the probe have an excellent linear relationship with Al³⁺ concentration range 25~55μmol/L, and the detection limit was calculated to be as low as 5.4×10^-9mol/L, which was far below the drinking water restriction of Al³⁺ by WHO (7.4×10^-6 mol/L). HRMS analysis showed the binding stoichiometry between probe and Al³⁺ was to be 2:1. Moreover, the probe was applied for biological detection in vivo of Al³⁺ ions, which presented excellent biocompatibility, and could be applied to the detection of Al³⁺ in water body and organisms.

导出引用

喻艳超, 冯俊凯, 刘波, 由君, 武文菊, 荆军凯. 一种turn-on型Al³⁺荧光探针及其活体生物检测[J]. 中国环境科学. 2020, 40(12): 5422-5427

YU Yan-chao, FENG Jun-kai, LIU Bo, YOU Jun, WU Wen-ju, JING Jun-kai. A “turn-on” fluorescent probe for Al³⁺ and its biological detection in vivo[J]. China Environmental Science. 2020, 40(12): 5422-5427

中图分类号： X835 X592

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